Mon, Oct 26, 2026Fall (Semester 1) · Week 10Day 44 of 6780-min block

Methods quiz

Today's target

Demonstrate mastery of PCR, restriction enzymes, gel electrophoresis, and microarrays on the methods quiz.

Due today · WebXam practice Required

Completed methods quiz plus a one-sentence correction for one missed or uncertain item.

Your 4 steps today
  1. 1
    Do this
    Demonstrate mastery of PCR, restriction enzymes, gel electrophoresis, and microarrays on the methods quiz.
  2. 2
  3. 3
    Submit this
    WebXam practice: Completed methods quiz plus a one-sentence correction for one missed or uncertain item.
  4. 4
    Submit it here
    1. 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
    2. 2Clever. Clever opens. Sign in if it asks.
    3. 3Microsoft (district) login. Use your district Microsoft account (the one for school).
    4. 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
    The file to submit is named: Genetics of Disease (Medical Interventions) › PCR, restriction enzymes, electrophoresis, microarrays, and the limits of each method. › WebXam practice
    Open Schoology
Were you absent? Jump to the make-up plan
Where this fits
Tested on (Ohio WebXam)
Genetics of Disease · 072130
PLTW lesson
MI · Methods quiz
WebXam domain
Bio-Molecular Technology
Evidence to produce
WebXam practice
Lab / skill
Genetic Science Learning Center: Gel Electrophoresis
Quick glossary
CER:
Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
SOP:
Standard Operating Procedure — the exact steps to follow (especially in a lab).
Tracker:
Your PLTW progress log where you record completed evidence.
myPLTW:
The PLTW course site where you do the online activities — you open it through Schoology.
Learn first

Minute-by-minute · 80-minute block

💡 Big idea: Can you choose the right molecular tool for a given diagnostic question and explain why?

  1. 0-20Review PCR diagram, gel table, and method comparison; address questions
  2. 20-55Take genetic testing methods quiz in course shell independently
  3. 55-70Write one-sentence correction for one missed or uncertain item
  4. 70-78Audit testing-methods unit tracker; confirm all benchmarks green
  5. 78-80Submit quiz and correction note; teacher confirms unit complete
Mr. Mendoza's 5-minute intro
  • Hook: Show one quiz-style scenario (which method would you use to detect a 500 bp deletion?) and solve it together.
  • Why it matters: These methods appear on the WebXam 072130 under the Molecular and Genetic Technology domain, the single largest content area.
  • Today's structure: 20 min review, 35 min quiz, 15 min correction, 10 min tracker close.
  • Exit goal: Quiz submitted and one correction note posted before the bell.
Do this, step by step
  1. 1Review your PCR diagram, gel interpretation, and method comparison from this week.
  2. 2Take the genetic testing methods quiz in the course shell.
  3. 3For one missed or uncertain item, write a one-sentence correction.
  4. 4Submit the quiz and your correction note for the week-end summative.
You'll be able to
  • You'll be able to identify the right method for a given testing goal.
  • You'll be able to interpret PCR and gel results under assessment.
Know by the end
  • PCR amplifies a targeted sequence; gel separates amplified fragments by size; microarray surveys genome-wide expression simultaneously.
  • Knowing which method to use requires matching the question (one gene? many genes? size of fragment?) to the method's output.
  • Molecular and Genetic Technology is the largest domain on the WebXam 072130; mastery here directly targets it.
Do the work

Your PLTW work today

Open this PLTW section today

PCR, restriction enzymes, electrophoresis, microarrays, and the limits of each method. · Methods quiz

Day 5 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.

Do this: Open the genetic testing methods quiz in myPLTW covering Activities 2.1.2, 3.1.4, and related content, and confirm all testing-methods activities are marked complete.

Complete

Mark the quiz activity complete after submission.

How far to get

Method comparison table should be done (Thursday); testing-methods unit at 100% today.

Upload as evidence

Screenshot of completed testing-methods progress page with quiz submitted.

All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.

The plan

Today's PLTW tracker

Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.

Use the code Mr. Mendoza gave you, not your name. Saved on this device.

PCR, restriction enzymes, electrophoresis, microarrays, and the limits of each method.Day 5 of this projectSee the full week plan
Today's PLTW target

PCR, restriction enzymes, electrophoresis, microarrays, and the limits of each method. · Methods quiz

Open the genetic testing methods quiz in myPLTW covering Activities 2.1.2, 3.1.4, and related content, and confirm all testing-methods activities are marked complete.

Method comparison table should be done (Thursday); testing-methods unit at 100% today.

This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.

1 · What you do today

🎯 Demonstrate mastery of PCR, restriction enzymes, gel electrophoresis, and microarrays on the methods quiz.

  • Review your PCR diagram, gel interpretation, and method comparison from this week.
  • Take the genetic testing methods quiz in the course shell.
  • For one missed or uncertain item, write a one-sentence correction.
  • Submit the quiz and your correction note for the week-end summative.
2 · Turn in today

WebXam practice: Completed methods quiz plus a one-sentence correction for one missed or uncertain item.

Submit on Schoology

Upload by 11:29 PM for full credit.

3 · Who's doing what (team)
TaskWho
Review your PCR diagram, gel interpretation, and method comparison from this week._______
Take the genetic testing methods quiz in the course shell._______
For one missed or uncertain item, write a one-sentence correction._______
Submit the quiz and your correction note for the week-end summative._______

Working solo? Put your own name in "Who" for every row.

4 · Words I can use correctly
5 · I'm successful today when I can…
  • You'll be able to identify the right method for a given testing goal.
  • You'll be able to interpret PCR and gel results under assessment.
6 · Reflection & next steps
Where are you today?0/6 checked
Pick your period and code first.
Explore

Teacher-posted resources

Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.

Use during lessonFor: Everyone
MI 2.1.2 PCR Lab Group Assignment & Protocol Guide
worksheet/handoutOpens here
Open the file

Open this when the class reaches this activity and use it to complete the required lesson artifact.

Placement rationale

Matched PCR, gel electrophoresis, microarrays by path:Medical-Interventions/Unit-2_How-to-Screen-Your-Genes/2.1_Genetic-Testing-and-Screening; keywords:pcr, gel electrophoresis. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

Catch-up / reteachFor: Need extra support
MI Unit 2 Student Review: Genetic Disorders & Gel Electrophoresis
worksheet/handoutOpens here
Open the file

Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.

Placement rationale

Matched PCR, gel electrophoresis, microarrays by path:Medical-Interventions/Unit-2_How-to-Screen-Your-Genes/00_Unit-Overview; keywords:pcr, gel electrophoresis. Score 138. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

Extension / challengeFor: Ready to go deeper
MI Activity 2.1.4 Genetic Testing (Optional)
worksheet/handoutOpens here
Open the file

Use this after the required lesson work when you are ready for a harder application or a deeper connection.

Placement rationale

Matched PCR, gel electrophoresis, microarrays by path:Medical-Interventions/Unit-2_How-to-Screen-Your-Genes/2.1_Genetic-Testing-and-Screening; keywords:gel electrophoresis. Score 134. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.

Lab day

Lab & supplies

Bring / set up
Agarose gel and casting trayGel electrophoresis chamber with power supplyMicropipette and tipsLoading dye and DNA size ladderTAE or TBE running bufferSafety goggles and nitrile gloves
Genetic Science Learning Center: Gel Electrophoresis
Words

This unit's vocabulary

primerrestriction enzymegel electrophoresismicroarray/MY-kroh-uh-ray/hybridizationmarker

Tap the speaker to hear a term. Weekly vocabulary task: add two of these terms to your notebook glossary with a definition and an example in your own words.

Check yourself

WebXam practice

Tap an answer to check it · nothing is recorded or graded
How many primers are required for a standard polymerase chain reaction (PCR)?
What is the correct order of the three steps of one PCR cycle?
In gel electrophoresis, a set of DNA fragments of known sizes used as a size reference for the unknown samples is called a
Restriction enzymes are used in genetic testing because they
Check yourself

Cumulative WebXam review

A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.

Tap an answer to check it · nothing is recorded or graded
[Review: Growing the evidence: aseptic culturing and superbug data] A single random mutation gives one bacterium a stronger cell wall that resists an antibiotic. How does this lead to a resistant infection?
[Review: Sound and shields: audiograms, the immune response, and vaccines] A vaccination works by activating the immune system so that a specialized cell can rapidly make antibodies on future exposure. What is that long-lasting cell called?
[Review: Reading the Family Tree: Genetic Testing Launch] A single nucleotide polymorphism (SNP) is best described as which of the following?
How many primers are required for a standard polymerase chain reaction (PCR)?
Explore

Where this leads — careers

What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.

Safety net

What to do if you were absent

If YOU are absent

Today is individual PLTW work, so do exactly what we did in class, from home: complete the same PLTW target above, then submit your WebXam practice.

Open Schoology (CMSD) and keep going

How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.

If MR. MENDOZA is absent

Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:

Genetic Science Learning Center: Gel Electrophoresis
Explore

Optional extra credit (async)

You've passed Unit 2, so the optional extra-credit track is open. Complete reserved-unit work from home (virtual labs included) for extra credit, all submitted on Schoology.

Open the extra-credit track
How this is graded
For: WebXam practice — Completed methods quiz plus a one-sentence correction for one missed or uncertain item.
  • Complete
    Every required part of the artifact is present, nothing left blank.
  • Accurate
    The science and the data are correct and match the evidence.
  • Scientific reasoning
    You explain your claim with evidence and reasoning (CER), not just an answer.
  • Professional communication
    Clear, organized, labeled, and written the way a clinician or scientist would.
  • Submitted
    Turned in the right way (Schoology for routine work) and confirmed.
Submission Zone

Drop your Mon, Oct 26, 2026 · Methods quiz here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).

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