Standard curve and lab prep
Build a standard curve from known dilutions and prepare the materials and steps for the ELISA model.
Standard curve (graph with labeled axes and best-fit line) plus written procedure for Thursday's model ELISA and labeled well layout.
- 1Do thisBuild a standard curve from known dilutions and prepare the materials and steps for the ELISA model.
- 2Use this resource
- 3Submit thisPre-lab: Standard curve (graph with labeled axes and best-fit line) plus written procedure for Thursday's model ELISA and labeled well layout.
- 4Submit it here
- 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
- 2Clever. Clever opens. Sign in if it asks.
- 3Microsoft (district) login. Use your district Microsoft account (the one for school).
- 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
The file to submit is named: Genetics of Disease (Medical Interventions) › Concentration, serial dilution, standard curves, antigen–antibody binding, direct vs. indirect ELISA. › Pre-labOpen Schoology
- CER:
- Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure — the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities — you open it through Schoology.
Minute-by-minute · 80-minute block
💡 Big idea: How does a graph of known standards let you determine the concentration of a completely unknown sample?
- 0-10 minCreate the concentration-vs-signal data table from Tuesday's dilution plan
- 10-25 minPlot the data points and draw the best-fit line; label both axes with units
- 25-40 minPractice reading the curve: trace from a given signal to a concentration; explain the process in writing
- 40-55 minPrepare the model ELISA station: label wells A through H (or per protocol); lay out dilution series
- 55-68 minWrite out the procedure steps for tomorrow's model run in numbered order
- 68-80 minPredict where a strongly positive sample would fall; compare prediction with a partner
- • A standard curve is how scientists turn a color into a number; without it, every signal is meaningless.
- • You built the dilution plan Tuesday; today you turn that plan into a graph and a physical setup.
- • Tomorrow's model ELISA depends entirely on what you prepare today.
- • Exit goal: a standard curve plotted on graph paper or a spreadsheet, and a labeled well layout ready for Thursday.
- 1Use your dilution series to make a table of concentration versus expected signal.
- 2Plot the points and draw the best-fit line that forms your standard curve.
- 3Explain how you would read an unknown sample's concentration off this curve.
- 4Prepare your ELISA-model station: label wells and lay out the dilution series.
- 5Write the procedure steps you will follow tomorrow in order.
- 6Predict where a strongly positive sample would fall on your curve.
- • You will be able to build and interpret a standard curve.
- • You will be able to read an unknown concentration from a curve.
- • You will be able to set up a labeled dilution series for ELISA.
- • A standard curve plots signal (y-axis) against known concentration (x-axis); the best-fit line lets you interpolate unknown concentrations.
- • Reading off a curve means finding the unknown's signal on the y-axis, tracing horizontally to the line, then reading down to the x-axis.
- • A well-labeled ELISA plate layout prevents errors that ruin an entire run.
Your PLTW work today
Concentration, serial dilution, standard curves, antigen–antibody binding, direct vs. indirect ELISA. · Standard curve and lab prep
Day 3 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.
Do this: Continue in Activity 1.1.4 Serial Dilutions in myPLTW; prepare to use your dilution series to build a standard curve for the ELISA.
Complete the antibody structure diagram and the standard-curve concept questions.
Dilution plan should be done (Tuesday); antibody diagram and standard-curve work due today.
Labeled antibody diagram with antigen-antibody binding annotated in notebook.
All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.
Today's PLTW tracker
Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.
Use the code Mr. Mendoza gave you, not your name. Saved on this device.
Concentration, serial dilution, standard curves, antigen–antibody binding, direct vs. indirect ELISA. · Standard curve and lab prep
Continue in Activity 1.1.4 Serial Dilutions in myPLTW; prepare to use your dilution series to build a standard curve for the ELISA.
Dilution plan should be done (Tuesday); antibody diagram and standard-curve work due today.
This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.
🎯 Build a standard curve from known dilutions and prepare the materials and steps for the ELISA model.
- Use your dilution series to make a table of concentration versus expected signal.
- Plot the points and draw the best-fit line that forms your standard curve.
- Explain how you would read an unknown sample's concentration off this curve.
- Prepare your ELISA-model station: label wells and lay out the dilution series.
- Write the procedure steps you will follow tomorrow in order.
- Predict where a strongly positive sample would fall on your curve.
Pre-lab: Standard curve (graph with labeled axes and best-fit line) plus written procedure for Thursday's model ELISA and labeled well layout.
Submit on SchoologyUpload by 11:29 PM for full credit.
| Task | Who |
|---|---|
| Use your dilution series to make a table of concentration versus expected signal. | _______ |
| Plot the points and draw the best-fit line that forms your standard curve. | _______ |
| Explain how you would read an unknown sample's concentration off this curve. | _______ |
| Prepare your ELISA-model station: label wells and lay out the dilution series. | _______ |
| Write the procedure steps you will follow tomorrow in order. | _______ |
| Predict where a strongly positive sample would fall on your curve. | _______ |
Working solo? Put your own name in "Who" for every row.
- You will be able to build and interpret a standard curve.
- You will be able to read an unknown concentration from a curve.
- You will be able to set up a labeled dilution series for ELISA.
Teacher-posted resources
Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched ELISA model, dilution, standard curve by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/1.1_The-Mystery-Infection; keywords:elisa, serial dilution, dilution. Score 154. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched ELISA model, dilution, standard curve by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/1.1_The-Mystery-Infection; keywords:elisa, serial dilution. Score 146. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched ELISA model, dilution, standard curve by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/1.1_The-Mystery-Infection; keywords:serial dilution, dilution. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.
Lab & supplies
This unit's vocabulary
Tap the speaker to hear a term. Weekly vocabulary task: add two of these terms to your notebook glossary with a definition and an example in your own words.
WebXam practice
Cumulative WebXam review
A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.
Where this leads — careers
What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.
What to do if you were absent
If you miss prep day, complete the assigned HHMI immunology virtual activity and build a standard curve from the teacher dilution dataset, then submit your graph and a read-off of one unknown.
HHMI BioInteractive (immunology resources)Then submit your Pre-lab on Schoology.
Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:
HHMI BioInteractive- CompleteEvery required part of the artifact is present, nothing left blank.
- AccurateThe science and the data are correct and match the evidence.
- Scientific reasoningYou explain your claim with evidence and reasoning (CER), not just an answer.
- Professional communicationClear, organized, labeled, and written the way a clinician or scientist would.
- SubmittedTurned in the right way (Schoology for routine work) and confirmed.
Drop your Tue, Sep 15, 2026 · Standard curve and lab prep here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).
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