Thu, Sep 10, 2026Fall (Semester 1) · Week 3Day 13 of 6780-min block

Pathogen-ID report submission

Today's target

Submit a complete pathogen-identification report defending your BLAST result with evidence.

Due today · Lab report Required

Pathogen-ID report: claim naming organism, reasoning paragraph citing E-value/query coverage/percent identity, control validation explanation, and one limitation sentence.

Your 4 steps today
  1. 1
    Do this
    Submit a complete pathogen-identification report defending your BLAST result with evidence.
  2. 2
  3. 3
    Submit this
    Lab report: Pathogen-ID report: claim naming organism, reasoning paragraph citing E-value/query coverage/percent identity, control validation explanation, and one limitation sentence.
  4. 4
    Submit it here
    1. 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
    2. 2Clever. Clever opens. Sign in if it asks.
    3. 3Microsoft (district) login. Use your district Microsoft account (the one for school).
    4. 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
    The file to submit is named: Genetics of Disease (Medical Interventions) › DNA identification, sequencing, BLAST, controls, query coverage, and E-value. › Lab report
    Open Schoology
Were you absent? Jump to the make-up plan
Where this fits
Tested on (Ohio WebXam)
Genetics of Disease · 072130
PLTW lesson
MI · Pathogen-ID report submission
WebXam domain
Bio-Molecular Technology
Evidence to produce
Lab report
Lab / skill
NCBI BLAST
Quick glossary
CER:
Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
SOP:
Standard Operating Procedure — the exact steps to follow (especially in a lab).
Tracker:
Your PLTW progress log where you record completed evidence.
myPLTW:
The PLTW course site where you do the online activities — you open it through Schoology.
Learn first

Minute-by-minute · 80-minute block

💡 Big idea: How does a scientist turn computational evidence into a defensible scientific claim?

  1. 0-10 minAssemble materials: hit-table screenshot, top hit data, control run result, and comparison table from Thursday
  2. 10-25 minDraft the Claim: one sentence naming the pathogen with full organism name
  3. 25-45 minWrite the Reasoning paragraph: cite E-value, query coverage, and percent identity; explain why each supports the claim
  4. 45-58 minAdd the control explanation: how the control run validates the workflow
  5. 58-70 minWrite the limitation sentence; proofread the full report for accuracy and completeness
  6. 70-80 minSubmit in the course shell; confirm it shows as turned in
Mr. Mendoza's 5-minute intro
  • This week you used the same public tool that public health labs use to identify outbreak pathogens: BLAST.
  • Today you write the report that would accompany that identification in a real investigation.
  • A good report explains not just what you found, but why you trust the result and where it could still be wrong.
  • Exit goal: report submitted and confirmed as turned in before the bell.
Do this, step by step
  1. 1Assemble your hit-table screenshot, top organism name, E-value, and query coverage.
  2. 2Write a Claim naming the pathogen and a Reasoning paragraph using your match-quality numbers.
  3. 3Explain how your control run shows the workflow was reliable.
  4. 4Add one sentence on a limitation of identifying a pathogen by sequence alone.
  5. 5Submit the report in the PLTW course shell.
  6. 6Confirm it is turned in and note what you would test to confirm in a real lab.
You'll be able to
  • You will be able to defend a pathogen ID with BLAST evidence.
  • You will be able to cite E-value and coverage to justify a match.
  • You will be able to state a limitation of sequence-based identification.
Know by the end
  • A credible identification report cites percent identity, E-value, and query coverage together, not just one number.
  • A control run proves the tool worked correctly; without it, the result cannot be trusted.
  • Sequence-based identification has limits: databases are incomplete, and contamination or sequencing errors can produce false matches.
Do the work

Your PLTW work today

Open this PLTW section today

DNA identification, sequencing, BLAST, controls, query coverage, and E-value. · Pathogen-ID report submission

Day 4 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.

Do this: Open the pathogen-ID report submission assignment in myPLTW for Activity 1.1.3 Using DNA to Identify Pathogens.

Complete

Submit the completed report with all required sections before end of class.

How far to get

Revised identification statement should be done (Thursday); report submitted and confirmed today.

Upload as evidence

Pathogen-ID report submission visible in the course shell.

All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.

The plan

Today's PLTW tracker

Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.

Use the code Mr. Mendoza gave you, not your name. Saved on this device.

DNA identification, sequencing, BLAST, controls, query coverage, and E-value.Day 4 of this projectSee the full week plan
Today's PLTW target

DNA identification, sequencing, BLAST, controls, query coverage, and E-value. · Pathogen-ID report submission

Open the pathogen-ID report submission assignment in myPLTW for Activity 1.1.3 Using DNA to Identify Pathogens.

Revised identification statement should be done (Thursday); report submitted and confirmed today.

This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.

1 · What you do today

🎯 Submit a complete pathogen-identification report defending your BLAST result with evidence.

  • Assemble your hit-table screenshot, top organism name, E-value, and query coverage.
  • Write a Claim naming the pathogen and a Reasoning paragraph using your match-quality numbers.
  • Explain how your control run shows the workflow was reliable.
  • Add one sentence on a limitation of identifying a pathogen by sequence alone.
  • Submit the report in the PLTW course shell.
  • Confirm it is turned in and note what you would test to confirm in a real lab.
2 · Turn in today

Lab report: Pathogen-ID report: claim naming organism, reasoning paragraph citing E-value/query coverage/percent identity, control validation explanation, and one limitation sentence.

Submit on Schoology

Upload by 11:29 PM for full credit.

3 · Who's doing what (team)
TaskWho
Assemble your hit-table screenshot, top organism name, E-value, and query coverage._______
Write a Claim naming the pathogen and a Reasoning paragraph using your match-quality numbers._______
Explain how your control run shows the workflow was reliable._______
Add one sentence on a limitation of identifying a pathogen by sequence alone._______
Submit the report in the PLTW course shell._______
Confirm it is turned in and note what you would test to confirm in a real lab._______

Working solo? Put your own name in "Who" for every row.

4 · Words I can use correctly
5 · I'm successful today when I can…
  • You will be able to defend a pathogen ID with BLAST evidence.
  • You will be able to cite E-value and coverage to justify a match.
  • You will be able to state a limitation of sequence-based identification.
6 · Reflection & next steps
Where are you today?0/9 checked
Pick your period and code first.
Explore

Teacher-posted resources

Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.

Use during lessonFor: Everyone
Activity 1.1.3 Using DNA to Identify Pathogens
worksheet/handoutOpens here
Open the file

Open this when the class reaches this activity and use it to complete the required lesson artifact.

Placement rationale

Matched Pathogen identification, PCR, sequencing, BLAST by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/1.1_The-Mystery-Infection; keywords:blast, sequencing, pathogen, dna, identification. Score 162. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

Extension / challengeFor: Ready to go deeper
POGIL: DNA Detective - BLAST Pathogen ID
reading/referenceOpens here
Open the file

Use this after the required lesson work when you are ready for a harder application or a deeper connection.

Placement rationale

Matched Pathogen identification, PCR, sequencing, BLAST by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/00_Unit-Overview; keywords:blast, pathogen, dna, identification. Score 150. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

Catch-up / reteachFor: Need extra support
MI 5-Day Review Day 1: Pathogen ID (ELISA, PCR, BLAST)
worksheet/handoutOpens here
Open the file

Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.

Placement rationale

Matched Pathogen identification, PCR, sequencing, BLAST by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/00_Unit-Overview; keywords:blast, pcr, pathogen, identification. Score 150. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.

Lab day

Lab & supplies

Bring / set up
Lab computers with internet accessNCBI BLAST nucleotide toolUnknown pathogen DNA sequence fileKnown control sequence fileShared results spreadsheetScreenshot tool
NCBI BLAST
Words

This unit's vocabulary

DNA sequencePCR(Polymerase Chain Reaction)Sanger sequencingBLAST(Basic Local Alignment Search Tool)E-valuequery coveragecontrol

Tap the speaker to hear a term. Weekly vocabulary task: add two of these terms to your notebook glossary with a definition and an example in your own words.

Check yourself

WebXam practice

Tap an answer to check it · nothing is recorded or graded
What is the best way to compare an unknown DNA sequence to a database of known sequences to identify a pathogen?
Bioinformatics is best defined as which of the following?
To isolate a bacterial pathogen's DNA from cerebrospinal fluid before sequencing, what is the correct early sequence of steps?
What was the landmark international collaboration that identified the nucleotide base pairs of humans?
Check yourself

Cumulative WebXam review

A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.

Tap an answer to check it · nothing is recorded or graded
[Review: Lab Safety & the Safety Data Sheet (SDS)] What does the abbreviation GLP stand for in a regulated biomedical laboratory?
[Review: Framing an Outbreak Investigation] Which microbiology principle states that one specific organism causes a specific disease and can be isolated from a host who has that disease?
What is the best way to compare an unknown DNA sequence to a database of known sequences to identify a pathogen?
Explore

Where this leads — careers

What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.

Safety net

What to do if you were absent

If YOU are absent

Today is individual PLTW work, so do exactly what we did in class, from home: complete the same PLTW target above, then submit your Lab report.

Open Schoology (CMSD) and keep going

How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.

If MR. MENDOZA is absent

Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:

NCBI BLAST
How this is graded
For: Lab report — Pathogen-ID report: claim naming organism, reasoning paragraph citing E-value/query coverage/percent identity, control validation explanation, and one limitation sentence.
  • Complete
    Every required part of the artifact is present, nothing left blank.
  • Accurate
    The science and the data are correct and match the evidence.
  • Scientific reasoning
    You explain your claim with evidence and reasoning (CER), not just an answer.
  • Professional communication
    Clear, organized, labeled, and written the way a clinician or scientist would.
  • Submitted
    Turned in the right way (Schoology for routine work) and confirmed.
Submission Zone

Drop your Thu, Sep 10, 2026 · Pathogen-ID report submission here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).

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