Thu, Sep 17, 2026Fall (Semester 1) · Week 4Day 18 of 6780-min block

Dilution and ELISA model submission

Today's target

Submit your standard curve, model ELISA data, and interpretation to close the dilution week.

Due today · Lab report Required

Standard curve graph, model ELISA data table (colors and concentrations), short interpretation of positive results, and one error sentence.

Your 4 steps today
  1. 1
    Do this
    Submit your standard curve, model ELISA data, and interpretation to close the dilution week.
  2. 2
  3. 3
    Submit this
    Lab report: Standard curve graph, model ELISA data table (colors and concentrations), short interpretation of positive results, and one error sentence.
  4. 4
    Submit it here
    1. 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
    2. 2Clever. Clever opens. Sign in if it asks.
    3. 3Microsoft (district) login. Use your district Microsoft account (the one for school).
    4. 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
    The file to submit is named: Genetics of Disease (Medical Interventions) › Concentration, serial dilution, standard curves, antigen–antibody binding, direct vs. indirect ELISA. › Lab report
    Open Schoology
Were you absent? Jump to the make-up plan
Where this fits
Tested on (Ohio WebXam)
Genetics of Disease · 072130
PLTW lesson
MI · Dilution and ELISA model submission
WebXam domain
Bio-Molecular Technology
Evidence to produce
Lab report
Lab / skill
HHMI BioInteractive
Quick glossary
CER:
Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
SOP:
Standard Operating Procedure — the exact steps to follow (especially in a lab).
Tracker:
Your PLTW progress log where you record completed evidence.
myPLTW:
The PLTW course site where you do the online activities — you open it through Schoology.
Learn first

Minute-by-minute · 80-minute block

💡 Big idea: How do scientists document a quantitative lab result so it can be reviewed, replicated, and trusted?

  1. 0-15 minFinalize the standard-curve graph: check axis labels, units, and best-fit line
  2. 15-30 minComplete the data table: well ID, observed color, assigned concentration for every well
  3. 30-50 minWrite the interpretation paragraph: name positive results and explain the evidence from the curve
  4. 50-62 minWrite the error sentence: name one specific source of uncertainty in reading colors by eye
  5. 62-72 minSubmit graph, data table, and interpretation to the course shell
  6. 72-80 minConfirm all items show as turned in; note one thing to do more carefully in the wet lab next week
Mr. Mendoza's 5-minute intro
  • The goal of any lab exercise is not just to run the procedure; it is to produce a record someone else can evaluate.
  • Today you finalize and submit everything from the dilution week: the standard curve, the data, and the interpretation.
  • Next week you run the real wet ELISA; what you notice about your errors today shapes how carefully you work then.
  • Exit goal: all three submission items confirmed as turned in before you leave.
Do this, step by step
  1. 1Finalize your standard-curve graph and label the axes clearly.
  2. 2Tabulate your model ELISA colors and the concentration you assigned each well.
  3. 3Write a short interpretation naming which samples were positive and how you knew.
  4. 4Add one sentence on a source of error in reading colors by eye.
  5. 5Submit your graph, data table, and interpretation in the PLTW course shell.
  6. 6Confirm it is turned in and note one thing you want to do more carefully in the wet lab.
You'll be able to
  • You will be able to submit a labeled standard curve and ELISA data.
  • You will be able to interpret model results against a curve.
  • You will be able to name a source of measurement error.
Know by the end
  • A complete submission packages the visual evidence (graph), the raw data (table), and the interpretation together.
  • Naming a source of error is part of good science; it shows you understand the limits of your own data.
  • Reviewing your model data before the wet ELISA helps you anticipate where precision matters most.
Do the work

Your PLTW work today

Open this PLTW section today

Concentration, serial dilution, standard curves, antigen–antibody binding, direct vs. indirect ELISA. · Dilution and ELISA model submission

Day 5 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.

Do this: Open the ELISA pre-lab submission in myPLTW for Activity 1.1.5 ELISA and confirm your standard-curve work is ready for next week's wet lab.

Complete

Submit your complete ELISA pre-lab packet: dilution plan, antibody diagram, and standard-curve table.

How far to get

Standard-curve table should be done (Thursday); full pre-lab packet submitted today.

Upload as evidence

Pre-lab packet submission visible in the course shell.

All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.

The plan

Today's PLTW tracker

Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.

Use the code Mr. Mendoza gave you, not your name. Saved on this device.

Concentration, serial dilution, standard curves, antigen–antibody binding, direct vs. indirect ELISA.Day 5 of this projectSee the full week plan
Today's PLTW target

Concentration, serial dilution, standard curves, antigen–antibody binding, direct vs. indirect ELISA. · Dilution and ELISA model submission

Open the ELISA pre-lab submission in myPLTW for Activity 1.1.5 ELISA and confirm your standard-curve work is ready for next week's wet lab.

Standard-curve table should be done (Thursday); full pre-lab packet submitted today.

This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.

1 · What you do today

🎯 Submit your standard curve, model ELISA data, and interpretation to close the dilution week.

  • Finalize your standard-curve graph and label the axes clearly.
  • Tabulate your model ELISA colors and the concentration you assigned each well.
  • Write a short interpretation naming which samples were positive and how you knew.
  • Add one sentence on a source of error in reading colors by eye.
  • Submit your graph, data table, and interpretation in the PLTW course shell.
  • Confirm it is turned in and note one thing you want to do more carefully in the wet lab.
2 · Turn in today

Lab report: Standard curve graph, model ELISA data table (colors and concentrations), short interpretation of positive results, and one error sentence.

Submit on Schoology

Upload by 11:29 PM for full credit.

3 · Who's doing what (team)
TaskWho
Finalize your standard-curve graph and label the axes clearly._______
Tabulate your model ELISA colors and the concentration you assigned each well._______
Write a short interpretation naming which samples were positive and how you knew._______
Add one sentence on a source of error in reading colors by eye._______
Submit your graph, data table, and interpretation in the PLTW course shell._______
Confirm it is turned in and note one thing you want to do more carefully in the wet lab._______

Working solo? Put your own name in "Who" for every row.

4 · Words I can use correctly
5 · I'm successful today when I can…
  • You will be able to submit a labeled standard curve and ELISA data.
  • You will be able to interpret model results against a curve.
  • You will be able to name a source of measurement error.
6 · Reflection & next steps
Where are you today?0/9 checked
Pick your period and code first.
Explore

Teacher-posted resources

Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.

Catch-up / reteachFor: Need extra support
MI 1.1.5 Serial Dilutions student resource sheet
worksheet/handoutOpens here
Open the file

Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.

Placement rationale

Matched ELISA model, dilution, standard curve by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/1.1_The-Mystery-Infection; keywords:elisa, serial dilution, dilution. Score 154. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

Use during lessonFor: Everyone
Activity 1.1.5 ELISA (full activity)
worksheet/handoutOpens here
Open the file

Open this when the class reaches this activity and use it to complete the required lesson artifact.

Placement rationale

Matched ELISA model, dilution, standard curve by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/1.1_The-Mystery-Infection; keywords:elisa, serial dilution. Score 146. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

Catch-up / reteachFor: Need extra support
MI 1.1.5 Student Resource Sheet Serial Dilutions
worksheet/handoutOpens here
Open the file

Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.

Placement rationale

Matched ELISA model, dilution, standard curve by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/1.1_The-Mystery-Infection; keywords:serial dilution, dilution. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.

Lab day

Lab & supplies

Bring / set up
Micropipettes and tipsMicrocentrifuge tubes or microplateStock antigen solutionBuffer or diluentMicroplate or tube rackLab notebook for the dilution table
HHMI BioInteractive
Words

This unit's vocabulary

antigen/AN-tih-jen/antibody/AN-tih-bod-ee/ELISA(Enzyme-Linked Immunosorbent Assay)/ee-LY-zuh/serial dilutionstandard curvesubstrateabsorbance

Tap the speaker to hear a term. Weekly vocabulary task: add two of these terms to your notebook glossary with a definition and an example in your own words.

Check yourself

WebXam practice

Tap an answer to check it · nothing is recorded or graded
An antigen is best described as which of the following?
Antibodies are produced by which type of leukocyte, and what is their main job?
In an ELISA, a darker color in the well indicates what about the antigen being tested?
A technician makes a serial dilution starting with 100 ng/mL of antigen, transferring equal parts antigen and water at each step. What is the concentration after the first two dilutions?
Check yourself

Cumulative WebXam review

A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.

Tap an answer to check it · nothing is recorded or graded
[Review: Lab Safety & the Safety Data Sheet (SDS)] What does the abbreviation GLP stand for in a regulated biomedical laboratory?
[Review: Framing an Outbreak Investigation] Which microbiology principle states that one specific organism causes a specific disease and can be isolated from a host who has that disease?
[Review: Who is the culprit? Identifying a pathogen with DNA and BLAST] What was the landmark international collaboration that identified the nucleotide base pairs of humans?
An antigen is best described as which of the following?
Explore

Where this leads — careers

What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.

Safety net

What to do if you were absent

If YOU are absent

Today is individual PLTW work, so do exactly what we did in class, from home: complete the same PLTW target above, then submit your Lab report.

Open Schoology (CMSD) and keep going

How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.

If MR. MENDOZA is absent

Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:

HHMI BioInteractive
How this is graded
For: Lab report — Standard curve graph, model ELISA data table (colors and concentrations), short interpretation of positive results, and one error sentence.
  • Complete
    Every required part of the artifact is present, nothing left blank.
  • Accurate
    The science and the data are correct and match the evidence.
  • Scientific reasoning
    You explain your claim with evidence and reasoning (CER), not just an answer.
  • Professional communication
    Clear, organized, labeled, and written the way a clinician or scientist would.
  • Submitted
    Turned in the right way (Schoology for routine work) and confirmed.
Submission Zone

Drop your Thu, Sep 17, 2026 · Dilution and ELISA model submission here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).

Upload a project