Cloning workflow quiz
You have run each piece this week, but on the quiz can you put cut, insert, ligate, transform, select, express, and purify in the exact order and name what tool each step needs?
Demonstrate mastery of the cloning and expression on the weekly quiz.
- • You'll be able to sequence the cloning correctly.
- • You'll be able to apply cloning vocabulary to quiz scenarios.
- From memory, list the cloning in order using these words: cut, insert, ligate, transform, select, express, purify.
- Match a tool to a step: which step uses , and which step uses an ?
- 1Review your cloning-tools diagram, data, and expression notes.
- 2Open the cloning quiz in the PLTW course shell.
- 3Answer each item using the terms , , , and expression.
- 4Check that any ordered-step questions follow the correct sequence.
- 5Submit your completed cloning quiz.
🛠 Get unstuck · pick your level
🔑 Today's words · 5
Tap a word in the lesson for a plain meaning and one example. Recycled into next week's Do-Now.
Do the work · 80-minute blockfirst 5 min = hook▸
💡 Big idea: You have mastered the cloning when you can order every step and name its tool and failure mode, because a pipeline breaks precisely at whatever step you cannot sequence or explain.
- 0-15Silent review: cloning-tools diagram, data, expression notes
- 15-20Open quiz in PLTW course shell; read all items before answering
- 20-55Complete quiz items; use required vocabulary for every response
- 55-65Check ordered-step questions against sequence
- 65-72Final review pass before submit
- 72-80Submit quiz; note any items to revisit for WebXam prep
- • Today is the knowledge checkpoint for everything you did this week.
- • The quiz will ask you to sequence steps, apply vocabulary, and reason about results.
- • Spend the first 15 minutes reviewing before you open the quiz.
- • The cloning appears in the Molecular and Genetic Technology and Lab SOPs domains of the WebXam.
- • The canonical is: cut, insert, ligate, transform, select, express, purify.
- • Each step has a required tool and a failure mode worth knowing.
- • Vocabulary precision on ordered-step questions separates partial from full credit.
Plasmids, restriction enzymes, ligase, transformation, protein expression. · Cloning quiz
Day 5 of this lesson. Open this exact section in myPLTW (find it in Clever, Microsoft sign-in), then do the work below.
Do this: Open the cloning quiz in myPLTW and confirm all cloning-unit evidence slots are filled before submitting.
Mark the quiz entry complete after submission.
Expression notes should be done (Thursday); cloning unit fully closed today.
Quiz submission confirming the cloning-unit evidence is complete.
All PLTW activities are completed inside the PLTW course environment: this page only gives direction. Submit producibles on Schoology.
Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.
Use the code Mr. Mendoza gave you, not your name. Saved on this device.
Plasmids, restriction enzymes, ligase, transformation, protein expression. · Cloning workflow quiz
Open the cloning quiz in myPLTW and confirm all cloning-unit evidence slots are filled before submitting.
Expression notes should be done (Thursday); cloning unit fully closed today.
This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.
🎯 Demonstrate mastery of the cloning and expression on the weekly quiz.
- Review your cloning-tools diagram, data, and expression notes.
- Open the cloning quiz in the PLTW course shell.
- Answer each item using the terms , , , and expression.
- Check that any ordered-step questions follow the correct sequence.
- Submit your completed cloning quiz.
WebXam practice: Completed cloning quiz using , , , and expression vocabulary with correctly ordered workflow steps.
Turn it in on Schoology using the checklist just below. Upload by 11:29 PM for full credit.
| Task | Who |
|---|---|
| Review your cloning-tools diagram, data, and expression notes. | _______ |
| Open the cloning quiz in the PLTW course shell. | _______ |
| Answer each item using the terms , , , and expression. | _______ |
| Check that any ordered-step questions follow the correct sequence. | _______ |
| Submit your completed cloning quiz. | _______ |
Working solo? Put your own name in "Who" for every row.
- You'll be able to sequence the cloning correctly.
- You'll be able to apply cloning vocabulary to quiz scenarios.
- 1Do thisDemonstrate mastery of the cloning and expression workflow on the weekly quiz.
- 2Use this resource
- 3Submit thisWebXam practice: Completed cloning workflow quiz using plasmid, ligase, transformation, and expression vocabulary with correctly ordered workflow steps.
- 4Submit it here
- 1Open Clever.
- 2Microsoft (district) sign-in.
- 3Schoology and myPLTW are both in Clever.
Look for this assignment in Schoology: Genetics of Disease (Medical Interventions) › Plasmids, restriction enzymes, ligase, transformation, protein expression. › WebXam practiceOpen Schoology
Learn it · deck, reading, and vocabulary▸
Tier 1 is the time-boxed teacher set for the block; Tier 2 adds scaffolded vocabulary, examples, and a reading routine; Tier 3 extends into careers and current biomedical applications.
Generated from this lesson's canonical data with a red-team citation check.
Students often think Students think that as long as they name the right steps, the order does not matter much for credit.. The trap: On ordered-step questions, sequence is the answer. Selecting before transforming, or purifying before expressing, is wrong even if every word is present. If you treat the steps as an unordered list, you lose exactly the points the quiz is testing.
Worked sample responses:
- Order the workflow: cut, insert, ligate, transform, select, express, purify. (You cut the plasmid and gene first, ligate the gene in, transform host cells, select the ones that took it up, let them express the protein, then purify it.)
- Match the tool: ligase seals the gene into the plasmid; a restriction enzyme makes the cuts; antibiotic does the selecting.
- Define transformation: getting the recombinant plasmid into host cells, often by heat shock or electroporation.
- Define expression: the host cell transcribing and translating the inserted gene to make the target protein.
Self-check: I made sure every ordered-step answer follows cut, insert, ligate, transform, select, express, purify, because vocabulary precision and correct order are what separate partial from full credit.
Also due today: Submit your completed cloning workflow quiz in the PLTW course shell.
- CER:
- Claim, Evidence, Reasoning: make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure, the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities. Find it in Clever with your Microsoft sign-in, right next to Schoology.
Tap the speaker to hear a term. Add two of these to your notebook glossary with a definition and an example in your own words.
Pick just 2 or 3 words from today and make them yours: write what each one means in your own words, then give one example from what you actually did in Cloning workflow quiz. Try your own words first; the glossary is there if you get stuck. This is voluntary and counts as extra credit, so keep it short.
Saved on this device. Show Mr. Mendoza or add these to your notebook glossary to claim the extra credit.
Classroom documents for this lesson are posted in Schoology. Open Schoology and find each one by the name shown on its card.
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched and cloning by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:, pglo. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched and cloning by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:, pglo. Score 138. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched and cloning by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:, pglo. Score 138. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
How to get there: open Clever and sign in with your Microsoft (district) account. You will find both Schoology and myPLTW right there in Clever. Turn in your work on Schoology; do the online activities in myPLTW.
Check yourself · commit, then reveal▸
Put these steps in the correct order and name the tool or condition each one requires: transform, cut, purify, ligate, select, insert, express.
Write an answer and pick a confidence to unlock the key.
Fast retrieval with instant answers, not the commit-then-reveal check above. Try each from memory first: write what you remember about the earlier units, then check yourself here.
Go further and get help▸
What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.
Today is individual PLTW work, so do exactly what we did in class, from home: complete the same PLTW target above, then submit your WebXam practice.
Open Schoology (CMSD) and keep goingHow to get there: open Clever and sign in with your Microsoft (district) account. You will find both Schoology and myPLTW right there in Clever. Turn in your work on Schoology; do the online activities in myPLTW.
Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:
Genetic Science Learning Center: CloningYou've passed Unit 2, so the optional extra-credit track is open. Complete reserved-unit work from home (virtual labs included) for extra credit, submitted on Schoology.
Open the extra-credit track- CompleteEvery required part of the artifact is present, nothing left blank.
- AccurateThe science and the data are correct and match the evidence.
- Scientific reasoningYou explain your claim with evidence and reasoning (CER), not just an answer.
- Professional communicationClear, organized, labeled, and written the way a clinician or scientist would.
- SubmittedTurned in the right way (Schoology for routine work) and confirmed.