SDS-PAGE gel results
Read an SDS-PAGE gel to judge the size and purity of your isolated protein.
Annotated SDS-PAGE gel image with labeled marker lane, estimated protein size, band counts by fraction, most-pure fraction identified, and a QC statement.
- 1Do thisRead an SDS-PAGE gel to judge the size and purity of your isolated protein.
- 2Use this resource
- 3Submit thisData table: Annotated SDS-PAGE gel image with labeled marker lane, estimated protein size, band counts by fraction, most-pure fraction identified, and a QC statement.
- 4Submit it here
- 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
- 2Clever. Clever opens. Sign in if it asks.
- 3Microsoft (district) login. Use your district Microsoft account (the one for school).
- 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
The file to submit is named: Genetics of Disease (Medical Interventions) › GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. › Data tableOpen Schoology
- CER:
- Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure — the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities — you open it through Schoology.
Minute-by-minute · 80-minute block
💡 Big idea: SDS-PAGE translates a protein mixture into a pattern of bands that reveals size and contamination level.
- 0-10Read gel-interpretation notes; define marker lane and band
- 10-28Annotate gel image: label marker lane, estimate target protein size
- 28-45Compare fraction lanes; count extra bands per lane
- 45-58Identify most-pure fraction; justify with band count
- 58-70Write QC statement: passed or failed purity goal with evidence
- 70-80Add annotated gel to tracker; preview Friday lab report
- • You collected fractions yesterday; today you find out what is actually in them.
- • SDS-PAGE separates proteins by size and makes every contaminant visible as a band.
- • A clean gel with one band says your purification worked; extra bands say it did not.
- • Gel interpretation is a core Lab SOPs skill tested on the 072130 WebXam.
- 1Read the gel-interpretation notes in the PLTW course shell and define the protein marker lane.
- 2Compare your fraction lanes to the marker to estimate your protein's size.
- 3Decide which fraction is most pure based on how few extra bands it shows.
- 4Write one QC statement on whether the purification met the purity goal.
- 5Add your annotated gel reading to your Unit 4 PLTW tracker evidence.
- • You'll be able to estimate protein size against a marker lane.
- • You'll be able to judge purity and write a QC statement from a gel.
- • SDS denatures proteins and gives all of them a uniform negative charge proportional to size.
- • Smaller proteins migrate farther through the gel matrix in a given time.
- • A pure target fraction shows one dominant band at the expected molecular weight.
Your PLTW work today
GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. · SDS-PAGE gel results
Day 4 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.
Do this: Open Activity 4.1.4 Protein Electrophoresis in myPLTW and interpret your SDS-PAGE gel to judge size and purity of the isolated protein.
Mark the SDS-PAGE entry complete and attach your annotated gel image and QC statement.
Fraction-collection data should be done (Wednesday); annotated gel and QC statement due today.
Annotated SDS-PAGE gel with marker lane, size estimate, band counts, and QC statement submitted.
All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.
Today's PLTW tracker
Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.
Use the code Mr. Mendoza gave you, not your name. Saved on this device.
GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. · SDS-PAGE gel results
Open Activity 4.1.4 Protein Electrophoresis in myPLTW and interpret your SDS-PAGE gel to judge size and purity of the isolated protein.
Fraction-collection data should be done (Wednesday); annotated gel and QC statement due today.
This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.
🎯 Read an SDS-PAGE gel to judge the size and purity of your isolated protein.
- Read the gel-interpretation notes in the PLTW course shell and define the protein marker lane.
- Compare your fraction lanes to the marker to estimate your protein's size.
- Decide which fraction is most pure based on how few extra bands it shows.
- Write one QC statement on whether the purification met the purity goal.
- Add your annotated gel reading to your Unit 4 PLTW tracker evidence.
Data table: Annotated SDS-PAGE gel image with labeled marker lane, estimated protein size, band counts by fraction, most-pure fraction identified, and a QC statement.
Submit on SchoologyUpload by 11:29 PM for full credit.
| Task | Who |
|---|---|
| Read the gel-interpretation notes in the PLTW course shell and define the protein marker lane. | _______ |
| Compare your fraction lanes to the marker to estimate your protein's size. | _______ |
| Decide which fraction is most pure based on how few extra bands it shows. | _______ |
| Write one QC statement on whether the purification met the purity goal. | _______ |
| Add your annotated gel reading to your Unit 4 PLTW tracker evidence. | _______ |
Working solo? Put your own name in "Who" for every row.
- You'll be able to estimate protein size against a marker lane.
- You'll be able to judge purity and write a QC statement from a gel.
Teacher-posted resources
Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched Protein purification and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:protein purification, gfp, chromatography. Score 150. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched Protein purification and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:protein purification, gfp, chromatography. Score 150. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched Protein purification and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:gfp, chromatography. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.
Lab & supplies
- • No new chemical hazards today; gel image analysis is a paper or digital exercise.
- • If handling a physical stained gel, wear gloves as Coomassie stain is a skin irritant.
- • Dispose of any staining waste according to lab guidelines.
WebXam practice
Cumulative WebXam review
A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.
Where this leads — careers
What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.
What to do if you were absent
Today is individual PLTW work, so do exactly what we did in class, from home: complete the same PLTW target above, then submit your Data table.
Open Schoology (CMSD) and keep goingHow to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.
Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:
Genetic Science Learning Center: Genetics basics and proteinsOptional extra credit (async)
You've passed Unit 2, so the optional extra-credit track is open. Complete reserved-unit work from home (virtual labs included) for extra credit, all submitted on Schoology.
Open the extra-credit track- CompleteEvery required part of the artifact is present, nothing left blank.
- AccurateThe science and the data are correct and match the evidence.
- Scientific reasoningYou explain your claim with evidence and reasoning (CER), not just an answer.
- Professional communicationClear, organized, labeled, and written the way a clinician or scientist would.
- SubmittedTurned in the right way (Schoology for routine work) and confirmed.
Drop your Fri, Dec 4, 2026 · SDS-PAGE gel results here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).
Upload a project