Week 19 of 22
DECEMBER 1 TO 4
Fiction. No real student, family or grade appears.
The problem is separating one protein from thousands of others in the same tube, using whatever makes yours different: size, charge, or what it sticks to.
Green fluorescent protein, which is the perfect teaching molecule because you can literally see where it is. Purification stops being abstract when the fraction you want glows.
ME
You can see the fraction you want. It glows.
Running the column and collecting fractions. Watching the glow move down the column and come out in a specific set of tubes is the most satisfying thing this course has done.
Running the fractions on a gel to see what is actually in each one. Purity is visible as the other bands disappearing.
Fractions, gel image, yield calculation, and the late-collection error written into the limitations.