Purification overview
If a batch of comes out of bacteria mixed with thousands of other bacterial proteins, DNA, and cell membrane pieces, what has to be removed before a person can inject it, and why?
Explain why a manufactured must be purified and what means for a medicine.
- • You'll be able to explain why a medicine must be purified.
- • You'll be able to define and a risk of impurity.
- When bacteria are grown to make a human , name two things besides the target protein that end up in the mixture when the cells are broken open.
- Why might a patient's body react badly to a medicine that still has bacterial leftovers in it?
- 1Read the overview notes in the PLTW course shell and define .
- 2List what else is in the cell mixture besides the target .
- 3Explain why an impure medicine could be unsafe.
- 4Preview the steps you will model this week.
- 5Submit a short exit ticket defining and one risk of impurity.
🛠 Get unstuck · pick your level
🔑 Today's words · 5
Tap a word in the lesson for a plain meaning and one example. Recycled into next week's Do-Now.
Do the work · 80-minute blockfirst 5 min = hook▸
💡 Big idea: A manufactured must be purified because every contaminant left in the mixture gets injected into the patient and can trigger an immune reaction or weaken the drug.
- 0-10Read overview; define in own words
- 10-25List cell-lysate components besides target
- 25-42Explain risk of each contaminant type
- 42-58Preview this week's steps (, SDS-PAGE)
- 58-70Write exit ticket: definition and one impurity risk
- 70-80Submit exit ticket; preview Tuesday diagram
- • Your bacteria made the recombinant , but it is swimming in a soup of thousands of other molecules.
- • Before it can enter a patient, everything else has to be removed.
- • Today you learn what is in that soup and why impurity is not just a quality problem but a problem.
- • and QC connect to the Lab SOPs domain of the 072130 WebXam.
- • Cell lysate contains the target mixed with thousands of other bacterial proteins, DNA, and membrane fragments.
- • Contaminants in a therapeutic can provoke dangerous immune responses in patients.
- • is quantified by the ratio of target to total protein, often confirmed by gel analysis.
GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. · overview
Day 1 of this lesson. Open this exact section in myPLTW (find it in Clever, Microsoft sign-in), then do the work below.
Do this: Open the - unit tracker in myPLTW for Activity 4.1.3 GFP Protein Purification and read the purification overview notes.
Mark the -overview entry complete and attach your exit ticket.
Cloning unit should be fully closed; this overview opens the - unit.
Exit ticket defining and one impurity risk submitted to the course shell.
All PLTW activities are completed inside the PLTW course environment: this page only gives direction. Submit producibles on Schoology.
Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.
Use the code Mr. Mendoza gave you, not your name. Saved on this device.
GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. · Purification overview
Open the - unit tracker in myPLTW for Activity 4.1.3 GFP Protein Purification and read the purification overview notes.
Cloning unit should be fully closed; this overview opens the - unit.
This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.
🎯 Explain why a manufactured must be purified and what means for a medicine.
- Read the overview notes in the PLTW course shell and define .
- List what else is in the cell mixture besides the target .
- Explain why an impure medicine could be unsafe.
- Preview the steps you will model this week.
- Submit a short exit ticket defining and one risk of impurity.
Exit ticket: Short exit ticket defining and identifying one concrete risk of an impure medicine.
Turn it in on Schoology using the checklist just below. Upload by 11:29 PM for full credit.
| Task | Who |
|---|---|
| Read the overview notes in the PLTW course shell and define . | _______ |
| List what else is in the cell mixture besides the target . | _______ |
| Explain why an impure medicine could be unsafe. | _______ |
| Preview the steps you will model this week. | _______ |
| Submit a short exit ticket defining and one risk of impurity. | _______ |
Working solo? Put your own name in "Who" for every row.
- You'll be able to explain why a medicine must be purified.
- You'll be able to define and a risk of impurity.
- 1Do thisExplain why a manufactured protein must be purified and what purity means for a medicine.
- 2Use this resource
- 3Submit thisExit ticket: Short exit ticket defining purity and identifying one concrete safety risk of an impure protein medicine.
- 4Submit it here
- 1Open Clever.
- 2Microsoft (district) sign-in.
- 3Schoology and myPLTW are both in Clever.
Look for this assignment in Schoology: Genetics of Disease (Medical Interventions) › GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. › Exit ticketOpen Schoology
Learn it · deck, reading, and vocabulary▸
Tier 1 is the time-boxed teacher set for the block; Tier 2 adds scaffolded vocabulary, examples, and a reading routine; Tier 3 extends into careers and current biomedical applications.
Generated from this lesson's canonical data with a red-team citation check.
Students often think Students assume that if bacteria were engineered to make the target , the protein comes out clean because that is what the cells were told to produce.. The trap: The engineered is a tiny fraction of everything inside the cell. Breaking the cells open releases all of the bacterium's own proteins, DNA, and membranes at the same time, so the target starts out badly outnumbered and must be separated, not just harvested.
Definition of purity: purity is how much of the total protein in my sample is actually the target protein, rather than other proteins and cell parts. High purity means almost all of it is the protein I want.
What else is in the mixture: besides my target protein, the cell lysate contains thousands of other bacterial proteins, DNA, and broken membrane fragments.
One safety risk of impurity: if a protein medicine still contains bacterial contaminants, those contaminants can trigger a dangerous immune reaction in the patient, so an impure dose is not just less effective, it can be unsafe.
Also due today: Submit your exit ticket to the course shell before leaving class.
- CER:
- Claim, Evidence, Reasoning: make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure, the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities. Find it in Clever with your Microsoft sign-in, right next to Schoology.
Tap the speaker to hear a term. Add two of these to your notebook glossary with a definition and an example in your own words.
Pick just 2 or 3 words from today and make them yours: write what each one means in your own words, then give one example from what you actually did in Purification overview. Try your own words first; the glossary is there if you get stuck. This is voluntary and counts as extra credit, so keep it short.
Saved on this device. Show Mr. Mendoza or add these to your notebook glossary to claim the extra credit.
Classroom documents for this lesson are posted in Schoology. Open Schoology and find each one by the name shown on its card.
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:protein purification, gfp, . Score 150. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:protein purification, gfp, . Score 150. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:gfp, . Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
How to get there: open Clever and sign in with your Microsoft (district) account. You will find both Schoology and myPLTW right there in Clever. Turn in your work on Schoology; do the online activities in myPLTW.
Check yourself · commit, then reveal▸
A lab reports their protein prep is '30 percent pure.' What does that number mean, and is it ready to inject?
Write an answer and pick a confidence to unlock the key.
Fast retrieval with instant answers, not the commit-then-reveal check above. Try each from memory first: write what you remember about the earlier units, then check yourself here.
Go further and get help▸
What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.
Today is individual PLTW work, so do exactly what we did in class, from home: complete the same PLTW target above, then submit your Exit ticket.
Open Schoology (CMSD) and keep goingHow to get there: open Clever and sign in with your Microsoft (district) account. You will find both Schoology and myPLTW right there in Clever. Turn in your work on Schoology; do the online activities in myPLTW.
Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:
Genetic Science Learning Center: Genetics basics and proteinsYou've passed Unit 2, so the optional extra-credit track is open. Complete reserved-unit work from home (virtual labs included) for extra credit, submitted on Schoology.
Open the extra-credit track- CompleteEvery required part of the artifact is present, nothing left blank.
- AccurateThe science and the data are correct and match the evidence.
- Scientific reasoningYou explain your claim with evidence and reasoning (CER), not just an answer.
- Professional communicationClear, organized, labeled, and written the way a clinician or scientist would.
- SubmittedTurned in the right way (Schoology for routine work) and confirmed.