GFP and chromatography
Our target is invisible and mixed with thousands of others in a clear liquid, so how do we know where it is and how do we pull it out without pulling out everything else?
Explain how GFP and let you track and separate a target .
- • You'll be able to explain how GFP marks the target .
- • You'll be able to describe how separates and elutes proteins.
- How does attaching GFP to a target let you see where that protein is in a set of tubes?
- In affinity , if the target sticks to the resin and the other proteins do not, what happens to the other proteins when you rinse the column?
- 1Read the notes in the PLTW course shell and define chromatography and .
- 2Explain why GFP glowing under UV light marks where the target is.
- 3Diagram a binding to a column and then eluting in a chosen fraction.
- 4Predict which fraction should glow if worked.
- 5Submit a labeled diagram as PLTW tracker evidence.
🛠 Get unstuck · pick your level
🔑 Today's words · 5
Tap a word in the lesson for a plain meaning and one example. Recycled into next week's Do-Now.
Do the work · 80-minute blockfirst 5 min = hook▸
💡 Big idea: GFP makes an invisible trackable so that you can watch use binding affinity to hold the target while everything else washes away.
- 0-10Read notes; define chromatography and
- 10-25Explain GFP reporter mechanism; annotate UV signal
- 25-45Diagram binding to column resin and wash steps
- 45-58Add step to diagram; label target fraction
- 58-70Predict which numbered fraction glows; write prediction
- 70-80Submit labeled diagram to tracker; confirm lab readiness
- • Tomorrow you run the column, so today you need to understand what it is actually doing.
- • GFP is the tracking beacon: wherever it glows, your is.
- • is the separation engine: it holds your while everything else washes away.
- • Understanding affinity and is a Lab SOPs and Molecular Technology skill on the WebXam.
- • GFP fused to the target fluoresces green under UV light, revealing which fraction contains the protein.
- • Affinity uses a resin that binds the target specifically, letting other proteins wash through.
- • uses a competing molecule or change in buffer conditions to release the bound target .
GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. · GFP and
Day 2 of this lesson. Open this exact section in myPLTW (find it in Clever, Microsoft sign-in), then do the work below.
Do this: Open Activity 4.1.3 GFP in myPLTW and diagram how GFP and affinity track and separate the target protein.
Mark the GFP-and- entry complete and attach your labeled chromatography diagram.
overview exit ticket should be done (Monday); diagram due today.
Labeled diagram with binding, wash, , and GFP fraction prediction submitted.
All PLTW activities are completed inside the PLTW course environment: this page only gives direction. Submit producibles on Schoology.
Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.
Use the code Mr. Mendoza gave you, not your name. Saved on this device.
GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. · GFP and chromatography
Open Activity 4.1.3 GFP in myPLTW and diagram how GFP and affinity track and separate the target protein.
overview exit ticket should be done (Monday); diagram due today.
This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.
🎯 Explain how GFP and let you track and separate a target .
- Read the notes in the PLTW course shell and define chromatography and .
- Explain why GFP glowing under UV light marks where the target is.
- Diagram a binding to a column and then eluting in a chosen fraction.
- Predict which fraction should glow if worked.
- Submit a labeled diagram as PLTW tracker evidence.
Notebook check: Labeled diagram showing binding, wash, , fraction collection, and GFP signal prediction.
Turn it in on Schoology using the checklist just below. Upload by 11:29 PM for full credit.
| Task | Who |
|---|---|
| Read the notes in the PLTW course shell and define chromatography and . | _______ |
| Explain why GFP glowing under UV light marks where the target is. | _______ |
| Diagram a binding to a column and then eluting in a chosen fraction. | _______ |
| Predict which fraction should glow if worked. | _______ |
| Submit a labeled diagram as PLTW tracker evidence. | _______ |
Working solo? Put your own name in "Who" for every row.
- You'll be able to explain how GFP marks the target .
- You'll be able to describe how separates and elutes proteins.
- 1Do thisExplain how GFP and chromatography let you track and separate a target protein.
- 2Use this resource
- 3Submit thisNotebook check: Labeled chromatography diagram showing protein binding, wash, elution, fraction collection, and GFP signal prediction.
- 4Submit it here
- 1Open Clever.
- 2Microsoft (district) sign-in.
- 3Schoology and myPLTW are both in Clever.
Look for this assignment in Schoology: Genetics of Disease (Medical Interventions) › GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. › Notebook checkOpen Schoology
Learn it · deck, reading, and vocabulary▸
Tier 1 is the time-boxed teacher set for the block; Tier 2 adds scaffolded vocabulary, examples, and a reading routine; Tier 3 extends into careers and current biomedical applications.
Generated from this lesson's canonical data with a red-team citation check.
Students often think Students think GFP glowing green IS the , as if the green light itself separates the from the rest.. The trap: GFP is only a reporter, a tag that tells you where the target is. The actual separation is done by the resin binding the target while everything else washes through. GFP watches the process; it does not perform it.
Definitions:
- Chromatography: a method that separates proteins by how strongly they bind to a material (resin) in a column.
- Elution: releasing the bound target protein from the resin so it flows out into a collected fraction.
Why GFP helps: GFP is fused to the target protein and glows green under UV light, so wherever the green glow is, the target protein is. That lets me see an otherwise invisible protein.
The steps, labeled:
1. Load: the protein mixture is added to the column; the target binds the resin while other proteins start to wash through.
2. Wash: buffer rinses away the unbound contaminants.
3. Elute: a change in buffer releases the target protein into the collected fractions.
Prediction: if purification worked, the elution fractions are the ones that should glow green under UV, because that is where the target protein comes off the column.
Also due today: Attach your labeled chromatography diagram to your PLTW tracker and submit it to the course shell.
- CER:
- Claim, Evidence, Reasoning: make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure, the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities. Find it in Clever with your Microsoft sign-in, right next to Schoology.
Tap the speaker to hear a term. Add two of these to your notebook glossary with a definition and an example in your own words.
Pick just 2 or 3 words from today and make them yours: write what each one means in your own words, then give one example from what you actually did in GFP and chromatography. Try your own words first; the glossary is there if you get stuck. This is voluntary and counts as extra credit, so keep it short.
Saved on this device. Show Mr. Mendoza or add these to your notebook glossary to claim the extra credit.
Classroom documents for this lesson are posted in Schoology. Open Schoology and find each one by the name shown on its card.
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:protein purification, gfp, . Score 150. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:protein purification, gfp, . Score 150. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:gfp, . Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
How to get there: open Clever and sign in with your Microsoft (district) account. You will find both Schoology and myPLTW right there in Clever. Turn in your work on Schoology; do the online activities in myPLTW.
Check yourself · commit, then reveal▸
You load your mixture, rinse the column, and then add elution buffer. At which of those three steps does the target protein finally come off the resin, and why not sooner?
Write an answer and pick a confidence to unlock the key.
Fast retrieval with instant answers, not the commit-then-reveal check above. Try each from memory first: write what you remember about the earlier units, then check yourself here.
Go further and get help▸
What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.
Today is individual PLTW work, so do exactly what we did in class, from home: complete the same PLTW target above, then submit your Notebook check.
Open Schoology (CMSD) and keep goingHow to get there: open Clever and sign in with your Microsoft (district) account. You will find both Schoology and myPLTW right there in Clever. Turn in your work on Schoology; do the online activities in myPLTW.
Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:
Genetic Science Learning Center: Genetics basics and proteinsYou've passed Unit 2, so the optional extra-credit track is open. Complete reserved-unit work from home (virtual labs included) for extra credit, submitted on Schoology.
Open the extra-credit track- CompleteEvery required part of the artifact is present, nothing left blank.
- AccurateThe science and the data are correct and match the evidence.
- Scientific reasoningYou explain your claim with evidence and reasoning (CER), not just an answer.
- Professional communicationClear, organized, labeled, and written the way a clinician or scientist would.
- SubmittedTurned in the right way (Schoology for routine work) and confirmed.