Cloning workflow quiz
Mon, Dec 7, 2026 · Week 16 · Genetics of Disease (Medical Interventions)
Today's goal: Demonstrate mastery of the cloning and expression workflow on the weekly quiz.
What a finished product looks like
This is a model of the work you should turn in today. Use it to check your own: match the structure and the level of detail, do not copy it. Your data and wording should be your own.
Worked sample responses:
- Order the workflow: cut, insert, ligate, transform, select, express, purify. (You cut the plasmid and gene first, ligate the gene in, transform host cells, select the ones that took it up, let them express the protein, then purify it.)
- Match the tool: ligase seals the gene into the plasmid; a restriction enzyme makes the cuts; antibiotic does the selecting.
- Define transformation: getting the recombinant plasmid into host cells, often by heat shock or electroporation.
- Define expression: the host cell transcribing and translating the inserted gene to make the target protein.
Self-check: I made sure every ordered-step answer follows cut, insert, ligate, transform, select, express, purify, because vocabulary precision and correct order are what separate partial from full credit.
Also due today: Submit your completed cloning workflow quiz in the PLTW course shell.
WebXam problem for today's skill
One exam-style question that uses exactly what you practiced today. Try it before you reveal the answer, then read why each choice is right or wrong.
Tap an answer to see the full explanation. Nothing is recorded or graded.