Plasmids, restriction enzymes, ligase, transformation, protein expression.
Your PLTW coursework: Medical Interventions ▸ Unit 4: How to Prevail When Organs Fail ▸ Lesson 4.1 Manufacturing Human Proteins ▸ "Activity 4.1.2 Protein Factories"
What to do if absent- CER:
- Claim, Evidence, Reasoning: make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure, the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities. Find it in Clever with your Microsoft sign-in, right next to Schoology.
Use restriction enzymes, , and steps to plan how a gene is cloned into a and expressed.
- 1Open the virtual cloning task in the PLTW course shell and read the map before you start cutting.
- 2Put on goggles and gloves, then set up your cloning workstation with the provided and insert models.
- 3Use the restriction- step to cut both the and the gene insert at matching sites.
- 4Use to join the insert into the and write one sentence on what ligase actually does.
- 5Model by moving your recombinant into the host cell and note how you would select successful cells.
- 6Predict whether the gene will be expressed and write one line of evidence using the word expression.
- • You'll be able to describe how restriction enzymes and build recombinant DNA.
- • You'll be able to explain what does in cloning.
- • You'll be able to predict from a cloning plan.
Open any day for its full lesson, the work due that day, and guided notes.
Cloning and recording results, selection counts, fraction data, yield, and a quality observation.
Annotated notes tracing and of the inserted gene, two yield-affecting conditions, and one piece of expression evidence from sample data.
Labeled diagram showing binding, wash, , fraction collection, and GFP signal prediction.
Fraction-collection data sheet recording tube number, buffer applied, GFP signal (yes/no), and target-fraction labels plus one error-control note.
Annotated SDS-PAGE gel image with labeled marker lane, estimated size, band counts by fraction, most-pure fraction identified, and a QC statement.
- Today matters because cloning a gene is how labs make , vaccines, and the proteins that save lives.
- Goal for today: run the cloning from cut to and predict whether your gene will express.
- Tie back to Monday's allocation debate by asking who benefits when a can be mass-produced cheaply.
- Upload your cloning and prediction to the class site, where the grade is recorded.
Do this: Advance the Unit 4 cloning benchmark by submitting your completed cloning and expression prediction on the class site.
- • Restriction enzymes cut DNA at specific sites so an insert and can join.
- • seals the insert into the to form recombinant DNA.
- • moves the recombinant into a host cell for expression.
- • Sequence the steps of cloning a gene into a .
- • Predict whether a cloned gene will be expressed.
📋 Tracker evidence due this week: your completed virtual cloning and expression prediction posted in the PLTW course shell.
All PLTW activities are completed inside the PLTW course environment: this page only gives direction.
Use this chart to keep your place. Nothing on it is turned in through the portal.
| Day | Work | Turn in |
|---|---|---|
| Monday · Mon, Dec 7 | Cloning and purification workflow | Cloning and purification workflow data table recording transformation results, selection counts, fraction data, yield, and a quality observation. |
| Tuesday · Tue, Dec 8 | Protein expression | Annotated notes tracing transcription and translation of the inserted gene, two yield-affecting conditions, and one piece of expression evidence from sample data. |
| Wednesday · Wed, Dec 9 | GFP and chromatography | Labeled chromatography diagram showing protein binding, wash, elution, fraction collection, and GFP signal prediction. |
| Thursday · Thu, Dec 10 | Protein-purification lab | Fraction-collection data sheet recording tube number, buffer applied, GFP signal (yes/no), and target-fraction labels plus one error-control note. |
| Friday · Fri, Dec 11 | SDS-PAGE gel results | Annotated SDS-PAGE gel image with labeled marker lane, estimated protein size, band counts by fraction, most-pure fraction identified, and a QC statement. |
- M: engineered debate
- T: recombinant DNA flow
- W: shortened-day cloning check
- Th: expression notes
- F: quiz
Turn in: Plasmids, restriction enzymes, ligase, transformation, protein expression. weekly work due Cloning workflow quiz.
Go to Schoology to turn this in.
Submit one PDF. Put your first and last name in the document header. Name the file: FirstName LastName - Assignment Title - YYYY-MM-DD.pdf.
Open Schoology PDF upload helpIf you cannot get in, see Mr. Mendoza. Do not skip the work.
5 panels from the illustrated semester.
Fiction. There is no such student. The lessons, labs and dates are the real planned course; the student, the classmates and the conversations are invented.
This explainer accompanies the PLTW lab protocol: watch it before lab.
Most days, this class is your PLTW coursework: and PLTW is online and individual. So being out usually just means doing exactly what we did in class, from home.
Open Clever, then myPLTWSign in to Clever with your district Microsoft account to open Schoology or myPLTW. Follow today's posted steps. If myPLTW will not open, use the posted alternative and tell Mr. Mendoza. Turn in your completed work through the Schoology assignment.
You can't do those from home: do this instead: Virtual cloning.
Class still runs. A substitute will post today's plan: complete the online activity above; it's built to be self-guided. Need the concept taught without a teacher? Use this authoritative explainer:
Genetic Science Learning Center: Cloning
Classroom documents for this lesson are posted in Schoology. Open Clever, then Schoology, and find each one by the name shown on its card.
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched Recombinant DNA and cloning by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:, pglo. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched Recombinant DNA and cloning by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:, pglo. Score 138. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched Recombinant DNA and cloning by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:, pglo. Score 138. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Sign in to Clever with your district Microsoft account to open Schoology or myPLTW. Follow today's posted steps. If myPLTW will not open, use the posted alternative and tell Mr. Mendoza. Turn in your completed work through the Schoology assignment.