Protein-purification lab
Thu, Dec 10, 2026 · Week 16 · Genetics of Disease (Medical Interventions)
Today's goal: Run a protein-purification procedure and collect fractions to isolate the target protein.
What a finished product looks like
This is a model of the work you should turn in today. Use it to check your own: match the structure and the level of detail, do not copy it. Your data and wording should be your own.
I loaded the protein mixture, washed, then eluted while watching for the green GFP signal under UV. Here is my record.
Reading: tubes 1 and 2 were the flow-through and wash, with no glow, meaning contaminants washed off. Tubes 4 and 5 glowed green, so those are my target collection.
Error-control note: one source of error is room light washing out the faint UV glow, so I controlled for it by reading each tube in the same darkened spot with the same UV lamp distance, so my yes/no calls are consistent.
| Tube | Buffer applied | GFP signal | Label |
|---|---|---|---|
| 1 | Load flow-through | No | Discard |
| 2 | Wash | No | Discard |
| 4 | Elution | Yes | Target |
| 5 | Elution | Yes | Target |
Also due today: Submit your fraction-collection data sheet to the course shell before leaving lab.
WebXam problem for today's skill
One exam-style question that uses exactly what you practiced today. Try it before you reveal the answer, then read why each choice is right or wrong.
Tap an answer to see the full explanation. Nothing is recorded or graded.