Protein-purification lab
Thu, Dec 10, 2026 · Week 16 · Genetics of Disease (Medical Interventions)
Today's goal: Run a protein-purification procedure and collect fractions to isolate the target protein.
This is a model of the work you should turn in today. Use it to check your own: match the structure and the level of detail, do not copy it. Your data and wording should be your own.
I loaded the protein mixture, washed, then eluted while watching for the green GFP signal under UV. Here is my record.
Reading: tubes 1 and 2 were the flow-through and wash, with no glow, meaning contaminants washed off. Tubes 4 and 5 glowed green, so those are my target collection.
Error-control note: one source of error is room light washing out the faint UV glow, so I controlled for it by reading each tube in the same darkened spot with the same UV lamp distance, so my yes/no calls are consistent.
| Tube | Buffer applied | GFP signal | Label |
|---|---|---|---|
| 1 | Load flow-through | No | Discard |
| 2 | Wash | No | Discard |
| 4 | Elution | Yes | Target |
| 5 | Elution | Yes | Target |
This model shows the level of evidence and organization needed to complete: Completes the purification lab: a fraction-collection data sheet recording tube number, buffer applied, GFP signal, and target-fraction labels, plus one error-control note.
- Name the variables and include units.
- Enter observations without changing the raw values.
- Check labels, calculations, and patterns before interpreting the data.
Keep the structure. Replace the question, facts, measurements, and evidence. Then recheck units, vocabulary, and whether the conclusion goes beyond the evidence.
Also due today: Submit your fraction-collection data sheet to Schoology before leaving lab.
Turn in: Fraction-collection data sheet
Go to Schoology to turn this in.
Submit one PDF. Put your first and last name in the document header. Name the file: FirstName LastName - Assignment Title - YYYY-MM-DD.pdf.
Open Schoology PDF upload helpIf you cannot get in, see Mr. Mendoza. Do not skip the work.
Claim ceiling: Today's evidence supports a classroom claim about protein-purification lab. It cannot prove causation, diagnose a real patient, or justify action outside this room.
One exam-style question that uses exactly what you practiced today. Try it before you reveal the answer, then read why each choice is right or wrong.
Tap an answer to see the full explanation. Nothing is recorded or graded.
It builds this reusable test skill: Identifying target fractions from GFP signal during column purification.
- Name the concept or data pattern being tested.
- Cross out choices that violate that rule or the evidence.
- Justify the best remaining choice before checking the answer.