Transformation, antibiotic selection, plasmid extraction, digest, gel interpretation.
Your PLTW coursework: Biomedical Innovation ▸ Problem 6: Molecular Biology in Action ▸ "Project 6.1.2 Construction and Cloning of Recombinant DNA"
What to do if absent- CER:
- Claim, Evidence, Reasoning: make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure, the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities. Find it in Clever with your Microsoft sign-in, right next to Schoology.
Interpret a with selection, then read a restriction digest run on a gel against a .
- 1Review the provided plate images and identify which plate shows successful .
- 2Explain in one sentence how selection lets only transformed colonies grow.
- 3Count the colonies on the selection plate and record the number in your notebook.
- 4Examine the provided gel image and locate the lane.
- 5Compare your sample bands to the ladder and estimate each fragment size.
- 6Write one sentence stating whether the digest matches the expected pattern.
- • You will be able to identify successful using selection plates.
- • You will be able to use a to estimate fragment sizes.
- • You will be able to judge whether a gel matches an expected digest pattern.
Open any day for its full lesson, the work due that day, and guided notes.
Complete and gel laboratory notebook: transformation notes, plate counts with units, gel , fragment-size estimates with units, conclusion on identity, one error source, and one future improvement.
One sentence on the consequence of a broken , plus a written ethical standard statement for your own independent project.
evidence table with each item, collector, date and location of collection, all custody transfers with dates and handlers, storage and sealing method, and flagged documentation gaps.
Independent project definition: , testable claim, methods outline with identified variables and measurement units, a designated control, and one named limitation.
- Hook: a glowing plate and a few bands on a gel can confirm that you successfully moved a gene into bacteria.
- Today's goal: read real selection plates and gels like a working molecular biologist.
- Monday bioethics debate connects: how should labs contain genetically modified bacteria safely?
- Reminder: your graded gel interpretation is submitted on the class site.
Do this: Advance your PLTW molecular biology problem by completing your and gel interpretation in the online course shell.
- • selection allows only transformed cells carrying the resistance gene to grow.
- • A provides known fragment sizes for comparison on a gel.
- • Identify successful from selection plates.
- • Estimate DNA fragment sizes against a ladder on a gel.
📋 PLTW evidence due: a plate interpretation and an annotated gel analysis with fragment sizes in the course shell.
All PLTW activities are completed inside the PLTW course environment: this page only gives direction.
Use this chart to keep your place. Nothing on it is turned in through the portal.
| Day | Work | Turn in |
|---|---|---|
| Monday · Mon, May 10 | Notebook submit | Complete transformation and gel laboratory notebook: transformation notes, plate colony counts with units, gel standard curve, fragment-size estimates with units, conclusion on plasmid identity, one error source, and one future improvement. |
| Tuesday · Tue, May 11 | Research ethics debate | One sentence on the consequence of a broken chain of custody, plus a written ethical standard statement for your own independent project. |
| Wednesday · Wed, May 12 | Evidence for | |
| Thursday · Thu, May 13 | Forensic evidence table | Forensic evidence table with each item, collector, date and location of collection, all custody transfers with dates and handlers, storage and sealing method, and flagged documentation gaps. |
| Friday · Fri, May 14 | Project question | Independent project definition: researchable question, testable claim, methods outline with identified variables and measurement units, a designated control, and one named limitation. |
- M: biotech debate
- T:
- Th: gel map
- F: notebook submit
Turn in: Transformation, antibiotic selection, plasmid extraction, digest, gel interpretation. weekly work due Transformation and gel notebook.
Go to Schoology to turn this in.
Submit one PDF. Put your first and last name in the document header. Name the file: FirstName LastName - Assignment Title - YYYY-MM-DD.pdf.
Open Schoology PDF upload helpIf you cannot get in, see Mr. Mendoza. Do not skip the work.
4 panels from the illustrated semester.
Fiction. There is no such student. The lessons, labs and dates are the real planned course; the student, the classmates and the conversations are invented.
This explainer accompanies the PLTW lab protocol: watch it before lab.
Most days, this class is your PLTW coursework: and PLTW is online and individual. So being out usually just means doing exactly what we did in class, from home.
Open Clever, then myPLTWSign in to Clever with your district Microsoft account to open Schoology or myPLTW. Follow today's posted steps. If myPLTW will not open, use the posted alternative and tell Mr. Mendoza. Turn in your completed work through the Schoology assignment.
You can't do those from home: do this instead: Provided plate and gel images.
Class still runs. A substitute will post today's plan: complete the online activity above; it's built to be self-guided. Need the concept taught without a teacher? Use this authoritative explainer:
Learn.Genetics (University of Utah): gel electrophoresis
Classroom documents for this lesson are posted in Schoology. Open Clever, then Schoology, and find each one by the name shown on its card.
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched , , molecular evidence by path:Biomedical-Innovations/Problem-6_Molecular-Biology/6.1_Molecular-Biology; keywords:transformation, , molecular. Score 146. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched , , molecular evidence by path:Biomedical-Innovations/Problem-6_Molecular-Biology/6.1_Molecular-Biology; keywords:gel, molecular. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched , , molecular evidence by path:Biomedical-Innovations/Problem-6_Molecular-Biology/6.1_Molecular-Biology; keywords:transformation, molecular. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Sign in to Clever with your district Microsoft account to open Schoology or myPLTW. Follow today's posted steps. If myPLTW will not open, use the posted alternative and tell Mr. Mendoza. Turn in your completed work through the Schoology assignment.