Antibodies, and the test built from them

One lecture for all three classes: what a protein is, what an antibody is, how your body makes the right one, and how an ELISA turns all of that into a number you can measure.

How the block is spent: SEC 01 proteins 8 min, SEC 02 the antibody 12 min, SEC 03 specificity 10 min, SEC 04 how they are made 12 min, SEC 05 the immune system 10 min, SEC 06 how an ELISA works 14 min, SEC 07 what it is for 10 min.

  • Guided notesOpen this first

    Open this first and keep it open. Twenty lines to fill and four diagrams to label in your own hand.

  • The lecture handoutOpen beside your work

    The eight labelled figures the lecture follows. Open it if you miss something or you were away.

  • ELISA debate preparation manualYou write in this

    The week's last handout. Prepare both sides; you will not know yours until the day.

  • Study guide for next week's examOpen beside your work

    Everything on the exam, with what you must be able to DO for each idea. Nothing to hand in.

Every document here is the same file Mr. Mendoza prints, so a paper copy and this page have the same section numbers. If you were away, work from the same packet and say so on your notebook entry.

PBS/PBT · September 18 · D09 · Case A STATIC · Period 1-2

Whose Blood, Whose DNA

One product: Serology worksheet, Parts A–E plus your case's late-record log, in one worksheet.

This page has four parts, in the order you use them. Part 1 is what you need to know. Part 2 says what to do, in plain English. Part 3 shows the work done once on a practice sample that is not your case. Part 4 is your own case's observations, references and collection plan, the ten tasks, and the late record for the final ten minutes. Everything you write goes in one worksheet.

Start: Part 1, then follow the steps in Part 2. Digital deadline: September 18, 11:59 PM.

Part 1 of 4 · Background and definitions

What you need to know before you write

Why today matters: Two records can match on a shared feature without identifying a person. What must your worksheet show so another reader can see that limit?

The question you are answering: What can these supplied comparisons and records support, and what remains unknown?

D08 taught you to separate reported classifications, missing observations and inference. It did not supply a confirmed material identity or a DNA result.

Everything in your worksheet comes from six short readings. Read R1 to R4 before Part 2, and R5 and R6 when the tasks send you there: A1 uses R2 and A2 uses R1 and R3. B1 uses R2 and R3. B2 uses R3. B3 uses R5. C1 uses R4. D1 uses R2 and R6. E1 to E3 check R2, R3 and R4.

R1. Your one worksheet

Complete the serology worksheet, Parts A–E and your case's late-record log. Serology is the study of blood and other body fluids. Use the supplied illustrative records for your class's case. You are interpreting these records, not reporting tests or collection work you performed. Yesterday's screening classification does not establish a confirmed material identity. No DNA profile is supplied today.

Keep each evidence ID beside its observations and comparison. The reference summaries are supplied records, not reference cards you independently inspected. A record may give less information than you would like. Preserve that limit instead of filling the gap.

Used in A2.

R2. The ABO typing rule

An antigen is a marker an antibody can bind. Here, A and B antigens are markers on red blood cells. An antibody is a protein that binds a particular target. Agglutination means visible cell clumping caused by antibody binding. In this classroom model, anti-A serum contains antibodies to A markers; anti-B serum contains antibodies to B markers. These are the model's testing liquids, not the person's blood-type name.

Supplied anti-A observationSupplied anti-B observationClassroom ABO call
ClumpingNo clumpingA
No clumpingClumpingB
ClumpingClumpingAB
No clumpingNo clumpingO

The table above is the ABO typing rule. Use it only to interpret the supplied model. It does not validate a real blood-typing test. Type O means neither A nor B marker under the ABO typing rule, not that a red blood cell has no markers at all. NCBI ABO background [S1].

Used in A1, B1, D1, E1.

R3. Compare one feature and state its limit

ABO types are shared by many people. A matching type does not identify a donor. Compare the stain's classroom call with each supplied reference. The same call means they match on this one feature. A different call means they do not match under the ABO typing rule. Neither statement establishes where a person was, when material arrived, innocence or guilt.

If a reference says only other than B or other than O, keep those words. The exact alternative type is not supplied. A categorical summary can support a same/different comparison without supplying a more precise type.

DNA results can support a comparison with a reference, but conclusions depend on the actual results and their limits. No DNA profile is supplied for this worksheet, so do not make a DNA identification. NIJ comparison-result background [S2].

Used in A2, B1, B2, E2.

R4. Explain the reported collection plan

Moisture and storage conditions can affect how well biological evidence is preserved. Packaging requirements depend on the material and the approved procedure. For this paper exercise, describe the packaging and custody steps reported in your case. Do not say you performed them. Custody means the record of who handled an item. Use the collector and receiver roles, and mark unreported names or times not reported. The reported paper-envelope state is case information, not a universal packaging instruction. NIJ preservation background [S3].

Attached follicular tissue may provide DNA for testing. Follicular tissue is tissue from around a growing hair. Hairs without attached tissue can also contain DNA, but useful results depend on the sample and method. Appearance alone does not provide a DNA result. Record the tissue condition and plan stated in your own case; do not claim you inspected a specimen or established test success. NIJ-supported rootless-hair research [S4].

Used in C1, E3.

R5. Keep practice arithmetic separate

A reference range is a stated comparison interval. Keep its label and context attached. For the separate practice numbers, compare the given value with the lower and upper limits. To find points above the upper limit, subtract that upper limit from the value. To find percent of the upper limit, divide the value by the upper limit and multiply by 100.

Percent of the upper limit = practice value ÷ upper limit × 100.

This differs from percent above the upper limit. Use the requested label. The practice values have no supplied measurement unit, so do not add mg/dL. Sample P's 150 example and your 180 calculation are separate from the case's late record. The calculation does not diagnose a person.

Used in B3.

R6. Record the late information, without a clinical conclusion

A useful log keeps the sample ID, record label, units when supplied and relevant context. Some results are numbers; others are categories. Toxicology is the study of drugs and poisons. A glucose meter memory or a food-test category does not become a complete toxicology or cause-of-death conclusion.

Your case's late record is used in the final ten minutes. Follow only the matching case note below.

Used in D1.

Plain-English terms

Part D asks you to use two of these correctly: agglutination and toxicology. The rest appear in the readings and in your records.

Serology
The study of blood and other body fluids.
Supplied illustrative case record
You are interpreting these records, not reporting tests or collection work you performed.
Antigen
A marker an antibody can bind. Here, A and B antigens are markers on red blood cells.
Antibody
A protein that binds a particular target.
Agglutination
Visible cell clumping caused by antibody binding.
Anti-A and anti-B serum
In this classroom model, anti-A serum contains antibodies to A markers; anti-B serum contains antibodies to B markers. These are the model's testing liquids, not the person's blood-type name.
Classroom ABO call
The type the ABO typing rule (the R2 table) gives from two supplied observations. Use it only to interpret the supplied model. It does not validate a real blood-typing test. Type O means neither A nor B marker under the ABO typing rule, not that a red blood cell has no markers at all.
Other than B or other than O
If a reference says only other than B or other than O, keep those words. The exact alternative type is not supplied.
Same call and different call
The same call means they match on this one feature. A different call means they do not match under the ABO typing rule. Neither statement establishes where a person was, when material arrived, innocence or guilt.
Custody
The record of who handled an item. Use the collector and receiver roles, and mark unreported names or times not reported.
Follicular tissue
Tissue from around a growing hair. Hairs without attached tissue can also contain DNA, but useful results depend on the sample and method.
Reference range
A stated comparison interval. Keep its label and context attached.
Percent of the upper limit
Practice value ÷ upper limit × 100. This differs from percent above the upper limit.
Toxicology
The study of drugs and poisons. A glucose meter memory or a food-test category does not become a complete toxicology or cause-of-death conclusion.

The limit that applies to everything today

A matching type does not identify a donor. No DNA profile is supplied for this worksheet, so do not make a DNA identification. The finished worksheet does not claim a test, collection, DNA identity or clinical conclusion.

Part 2 of 4 · Directions in plain English

What you will do today, in order

Use supplied classroom typing observations to make limited comparisons, explain a reported collection plan and accurately log a case-specific late record.

You fill in one worksheet with five parts, A to E, plus the late-record log in the final ten minutes. The five steps below match that order. Each step says what to do, what to use and where to find it on this page.

  1. Step 1. Start in Part 1. Put your period and investigator code on the worksheet and label it supplied illustrative case record. Read R1 to R4, then answer Part A. A1 uses the ABO typing rule in R2, and you copy the first three words of the sentence you used. A2 uses R1 and R3.

  2. Step 2. Read the worked example in Part 3 before your own case. Sample P shows one type call made from two supplied observations, and two comparisons written with their limits, on a practice sample that is not your case. Then go to Part 4 and complete B1: fill in the model type call for each observation row from its supplied anti-A and anti-B results, then compare each of your two stain calls with all five reference summaries. Keep every evidence ID, and keep an other-than category when that is all the record gives.

  3. Step 3. Still in Part 4, write B2 and C1. B2 is a hypothetical, two sentences on what a same-type match agrees on and what it does not establish. C1 is a four-to-six-sentence explanation of one swab entry from your case's reported collection plan, using R4 for the preservation reason and the reported custody roles. Write not reported where a name or time is missing, and never invent a signature.

  4. Step 4. Finish the practice calculation and the checks: B3, the 180 practice value shown with the subtraction and 180 ÷ 99 × 100 and labeled percent of the upper limit; D1, one or two sentences using agglutination and toxicology; and E1 to E3. The 180 value and the B and O hypothetical are practice, not case evidence. Check that all ten task IDs are complete.

  5. Step 5. In the final ten minutes, log your case's late record on the last page of the worksheet, copying exactly what the record supplies and stating what it does not, then hand in the one worksheet on paper or as one PDF to your section assignment.

Record route: Supplied illustrative case record, available to all students. Complete one serology worksheet: Parts A–E plus your case's late-record log. Use your class's supplied illustrative records. Your work explains a classroom comparison and a reported collection plan; it does not claim that you collected or tested a sample.

Guided notes and the separate worked model are support, not extra submissions.

The 80-minute plan
  • 0–10 min (from 0:00), Teacher launch: Identify the one worksheet, your case and the paper-record boundary. Begin the briefing and A1–A2. Tasks A1, A2.
  • 10–25 min (from 0:10), One guided example: Read the ABO typing rule and the serum definitions, use Sample P and finish A1–A2. Tasks A1, A2.
  • 25–50 min (from 0:25), Individual work with teacher support: Complete B1's grid/comparisons, B2's hypothetical and C1's reported collection-plan explanation. Tasks B1, B2, C1.
  • 50–70 min (from 0:50), Continue the same worksheet: Complete B3, D1 and E1–E3; check all ten task IDs and prepare the late-record area. Tasks B3, D1, E1, E2, E3.
  • 70–77 min (from 1:10), Late record, then individual logging: Use only your case's released record and support card. Copy exact displays or line observations and reported categories, then state the limit. Tasks late-record.
  • 77–80 min (from 1:17), Check and submit: Check the late log and all Parts A–E. Hand in paper or one PDF; keep notebook support in your notebook. Tasks handoff.
Check and hand in

Your worksheet needs the model calls from the supplied observation rows, comparisons for both stain IDs, B2's two sentences, B3's calculations, C1's plan explanation, D1 and E1–E3, plus the correct late-record log. No unreported exact reference type, time, unit, signature or clinical conclusion should be added.

Hand the paper worksheet to Mr. Mendoza before leaving, or submit one PDF in Wk4 D09 · Whose Blood, Whose DNA (PLTW 1.1.4) by September 18 at 11:59 PM. Use your period and investigator code. Do not submit the same worksheet twice. Keep notebook pages in your notebook.

Working on paper? The complete handout has the same four parts and the response sheet, also as a PDF.

Slides and optional guided notes

The slides follow the same order as this page. Guided notes can support your work but are not another submission. Guided notes and the separate worked model are support, not extra submissions.

Download the editable PowerPoint or open it in Google Drive. These slides use a static sequence, with no click animations.

Optional support: Optional Guided Notes; Separate Sample P and 150 worked models; Existing voluntary vocabulary extra credit, kept separate from required D1.

Part 3 of 4 · Worked example

F1 and Sample P: a comparison done once, on a practice sample

Sample P is a training-kit sample, not evidence in either case, and its two practice references are not your references. Read it for the order of the work, because B1 asks for the same order with your own rows: read the two supplied observations, apply the ABO typing rule (R2) to make the type call, compare the call with each reference, and state the limit.

1. Read your own case record

Evidence ID + anti-A observation + anti-B observation

2. Apply the ABO typing rule

Make the model type call from the two supplied observations.

3. Compare the supplied reference

Same feature / different feature / information not supplied

Keep an "other than" category when that is all the record gives.

4. State the comparison limit

No donor identity, innocence, guilt or timeline conclusion.

5. Complete the same worksheet

Reported collection-plan explanation + separate practice calculation

6. Final ten minutes: your case's late record

Case A: exact display, time, supplied unit, labeled context; HI stays HI.

F1. Read, compare, record. Paper-record workflow. This is a teaching diagram, not a specimen image, blood-typing procedure or collection protocol.

Find your own-case evidence ID and both supplied clumping observations. Use the ABO typing rule in R2 to make the model type call. Compare that call with the supplied reference information. State agreement or disagreement only for this one feature. If information is missing, say so; do not fill an “other than” category with a guessed exact type. Add the comparison limit. Then finish the collection-plan explanation and separate arithmetic practice in the same worksheet. In the final ten minutes, log only your own case's late record. Preserve its display or category, source and missing context. The finished worksheet does not claim a test, collection, DNA identity or clinical conclusion.

Optional restart cue

Find the next unanswered task ID. Use R2 for the typing grid, R3 for comparisons, R4 for the collection-plan explanation, R5 for the practice calculation and R6 for the late record. Do not switch to the other case or create a second product.

Sample P: separate practice, not your case

This worked model uses the existing training-kit Sample P and two practice reference labels. It is not evidence for Case A or Case B. Use the structure, not these labels or answers, in your worksheet.

The supplied observations

The Sample P record describes grainy liquid with small dark clumps in the anti-A well, and smooth, even liquid in the anti-B well. In the supplied record that is anti-A yes, anti-B no. This is text describing the practice wells, not an image you personally examined or a test you ran.

Using the ABO typing rule in R2, anti-A clumping only gives the model call A. Practice Person 1's reference card is labeled A; Practice Person 2's card is labeled O.

The two comparisons, written with their limits

Practice comparisonSupplied informationModel conclusion
Sample P and Person 1A compared with ASame on this one classroom ABO feature; donor not identified
Sample P and Person 2A compared with ODifferent under the ABO typing rule; no conclusion about presence, timing, innocence or guilt

A complete comparison statement keeps both sides visible: “Sample P and Person 1 match on the supplied classroom ABO type A. That shared type does not identify the donor.” For Person 2: “Sample P's model type A differs from Person 2's stated type O. This is a mismatch in this one comparison, not proof about a person's actions.”

Your case may give a reference only as “other than B” or “other than O.” Keep that category in your comparison. Do not invent a specific replacement type.

The practice arithmetic, done once with 150

The existing made-up practice value is 150, with a supplied comparison range of 70 to 99. No measurement unit is supplied.

  1. Compare with the interval: 150 is above its upper limit of 99.
  2. Find the difference: 150 − 99 = 51 points above.
  3. Find percent of the upper limit: 150 ÷ 99 × 100 = about 152% of the upper limit.

The last label is “percent of,” not “percent above.” Your B3 task uses 180, not 150. Show your own calculation and keep this generic practice separate from any case reading or clinical interpretation.

The hypotheticals, kept separate

B2 imagines a stain and a reference with the same type. It is not another observation in your case. E2 imagines a type-B stain and a type-O reference; this is practice for both sections. Apply R3's one-feature comparison limit rather than using either hypothetical to change your case rows.

The model and these checks do not require another upload. Complete Parts A–E and your late-record log in the one serology worksheet.

Do not copy Sample P's labels or the 150 example. Your B3 uses 180. Your comparisons use your case's five reference summaries, including any other-than category exactly as the record gives it.

Next, Part 4 applies this same order to your case. Open this example on its own page if you want it beside your worksheet.

Part 4 of 4 · Your work

Your Case A records, the ten tasks and the late record

Everything below is yours to write. First come your case's supplied observation rows, the five reference summaries and the reported collection plan; they are the inputs for B1 and C1. Then the ten tasks, grouped into Parts A to E. Last, the late record your teacher releases in the final ten minutes.

4.1 Your Case A records

Supplied illustrative case record, available to all students. The source supplies reference summaries, not newly inspected original reference cards. A label other than a type does not supply the exact alternative type. Do not invent it. Use only your case.

Setting: Statik Sound, a neighborhood recording studio. Case number JH-26-0831-A. The frame each stain came out of is in your Case A evidence folder.

Observation grid: 4 supplied rows, model type call left blank for you

Row labelSupplied anti-ASupplied anti-BModel type call
E2-console: Console-corner stainNo (smooth well)Yes (grainy clumps)____
E2-floor: Floor stainNo (smooth well)Yes (grainy clumps)____
Victim, medical record (reference)NoYes____
Terrence Cole, donor record (reference)Yes (grainy clumps)No (smooth well)____

All five reference summaries, used for each stain

  • Victim, medical record (reference): anti-A No, anti-B Yes; type B reported in the case record.
  • Terrence Cole, donor record (reference): anti-A Yes (grainy clumps), anti-B No (smooth well); exact type: not supplied.
  • Vasquez: OTHER than B; exact type: not supplied.
  • Okoye: OTHER than B; exact type: not supplied.
  • Tran: OTHER than B; exact type: not supplied.

Same-type stain/reference comparison is a hypothetical prompt, not an additional match in either supplied case.

Reported collection plan and custody state

  • Console stain swab; evidence ID E2-console.
  • Floor stain swab; evidence ID E2-floor.
  • three can-rim swabs (one per energy-drink can); grouped under E4, count 3; individual new IDs: not supplied.
  • blanket hair H3 root check; existing label H3; root tissue reported available: no; recorded plan: no DNA swab from it.

References reported on file: reference swabs already on file for the victim and all four persons of interest.

This is the source's reported root check, not an independently inspected specimen. No general rootless-hair rule is inferred.

Reported custody state: Swab air-dried, sealed in a paper envelope (not plastic), labeled with the E-number, first custody row signed by the collector and the receiver.

Reported roles: collector and receiver. Collector identity: not reported. Receiver identity: not reported. Collection time: not reported. Transfer time: not reported. Actual signatures: not reported.

Reported illustrative packaging/custody state, not proof the student performed it or a complete approved collection protocol.

Practice numbers, not from the case

Practice numbers, not from the case: value 180, supplied range 70 to 99, measurement unit: not supplied. Requested: inside the supplied range?; points above the upper limit; percent of the upper limit: 180 / 99 × 100.

4.2 The ten tasks, in one worksheet

Complete the serology worksheet, Parts A–E and your case's late-record log. Serology is the study of blood and other body fluids. Use the supplied illustrative records for your class's case. You are interpreting these records, not reporting tests or collection work you performed. Yesterday's screening classification does not establish a confirmed material identity. No DNA profile is supplied today.

Write on the response sheet in the handout or on paper. The task letters match the sheet. Extra help sits under each task; it supports that same response and never adds a product.

Part A. Use the reading

Two short answers: A1 from the ABO typing rule in R2, quoting the sentence you used, and A2 from R1 and R3.

A1 PBS-U1-D09-A1

In one sentence, explain why clumping can occur with anti-A serum in this classroom model. Use the terms antibody and A marker. Copy the first three words of a supporting sentence in R2.

Use: Reading R2 primer and the ABO typing rule (Part 1)

Response fields:
  • Explanation
  • Supporting sentence starts

Space: 3 writing lines

Need a hint?

Anti-A names the model's testing liquid. It is not a blood-type label for the person.

A2 PBS-U1-D09-A2

Explain one limit of a same-type comparison and why today's worksheet cannot make a DNA identification.

Use: Reading R1 (Part 1); Reading R3 (Part 1)

Response fields:
  • Same-type limit
  • DNA-input limit

Space: 4 writing lines

Need a hint?

A shared feature is not a unique identity. Check which results are actually supplied.

Part B. Your case observations, then two practice items

B1 is the main work: a type call for each observation row from 4.1, then each of your two stain calls compared with all five references. B2 and B3 are practice, not case evidence.

B1 PBS-U1-D09-B1

Use the supplied anti-A and anti-B observations to fill the model type call for each observation row. Then compare each of your two stain calls with all five supplied reference summaries. Keep the stain ID, reference label, reported pattern or category, same/different result and limit together. Do not invent an exact type for an other-than category.

Use: your case's supplied observations and five reference summaries (4.1); Reading R2 (Part 1); Reading R3 (Part 1); Sample P model (Part 3)

Response fields (observation grid 4 rows; comparison grid 10 rows, five per stain):
  • Observation grid: row label, supplied anti-A, supplied anti-B, blank model type call
  • Comparison grid: stain ID, reference label, supplied reference pattern/category, blank same/different call, reason and limit
Need a hint?

Four observation rows are supplied in A and three in B, including references with well observations. All five reference summaries are still used for each stain. Missing exact types remain categorical.

B2 PBS-U1-D09-B2

Hypothetical practice, not a new case result: a stain and a person-of-interest reference have the same classroom ABO type. In two sentences, state what agrees and what that agreement does not establish.

Use: Prompt hypothetical; Reading R3 (Part 1)

Response fields:
  • Two-sentence comparison and limit

Space: 4 writing lines

Need a hint?

Do not add this hypothetical match to your case matrix.

B3 PBS-U1-D09-B3

Practice numbers, not from the case: a value is 180 and the supplied range is 70 to 99. Is it inside that interval? How many points above the upper limit is it? What percent of the upper limit is 180? Show the subtraction and 180 ÷ 99 × 100. Label percent of the upper limit, not percent above. No measurement unit is supplied.

Use: the practice numbers, not from the case (4.1); Reading R5 (Part 1); Separate 150 arithmetic model (Part 3)

Response fields:
  • Inside/outside
  • Difference calculation
  • Percent-of-upper-limit calculation and label

Space: 5 calculation/writing lines

Need a hint?

Use 99 as the upper limit for this practice. Do not use the case reference card or add mg/dL.

Optional single comparison check, not alternate work plans

A reference is labeled only other than B. What should the worksheet preserve?

  1. Choose a likely exact alternative type.
  2. Keep other than B and make only the supported classroom comparison.
  3. Use Sample P's reference type in its place.
Check your choice

A supplied category can support a limited comparison without supplying an exact alternative type.

  • This invents information missing from the reference summary.
  • This keeps the source's level of detail and the model's limit.
  • Practice references are a different dataset and cannot replace case information.

Part C. The reported collection plan

Four to six sentences explaining one swab entry from your case's plan in 4.1, using R4.

C1 PBS-U1-D09-C1

Choose one swab entry from your case's supplied collection-plan record. In four to six sentences, explain the reported item and location; its reported packaging and the preservation reason from R4; the collector/receiver roles and any unreported names or times; and the reference samples reported on file. This is a paper-record explanation, not instructions for performing collection or a claim that you packaged the sample.

Use: your case's reported collection plan (4.1); the reported custody state (4.1); Reading R4 (Part 1)

Response fields:
  • Chosen source label and existing evidence ID if supplied
  • Four-to-six-sentence explanation covering the four components

Space: 9 writing lines

Need a hint?

Do not invent an evidence ID for a lid or hair entry whose ID is not supplied, and do not write an actual signature. Use a listed swab entry for this response.

Part D. Use the vocabulary

One or two sentences that use agglutination and toxicology correctly. The definitions are in Part 1.

D1 PBS-U1-D09-D1

Use agglutination and toxicology in one or two sentences about this worksheet. Explain their roles without treating the late record as a diagnosis or a complete toxicology conclusion.

Use: Reading R2 (Part 1); Reading R6 general explanation (Part 1)

Response fields:
  • One or two applied sentences

Space: 3 writing lines

Need a hint?

The typing model and the study of drugs/poisons are different concepts. Do not invent a toxicology result.

Part E. Practice checks

Three short checks: the ABO typing rule (R2), a comparison with its limit (R3) and a preservation reason (R4). Checking is part of the work, not a second submission.

E1 PBS-U1-D09-E1

Practice check: the supplied observation says clumping with anti-A only. What is the classroom model call?

Use: Reading R2, the ABO typing rule (Part 1)

Response fields:
  • Model type call

Space: 1 writing line

Need a hint?

This is a check of the ABO typing rule, not another assigned stain.

E2 PBS-U1-D09-E2

Hypothetical practice for both cases: a stain has classroom type B and a reference has type O. Write the same/different comparison and one limit. Do not change your case records.

Use: Prompt hypothetical; Reading R3 (Part 1)

Response fields:
  • Comparison and limit

Space: 2 writing lines

Need a hint?

Limit the statement to this feature comparison, not a person's actions.

E3 PBS-U1-D09-E3

Why can moisture matter when preserving biological evidence? State the reason from R4 and keep real packaging decisions tied to the material and approved procedure.

Use: Reading R4 (Part 1)

Response fields:
  • Preservation reason and limit

Space: 2 writing lines

Need a hint?

Do not claim that one package is suitable for every specimen or that you performed the reported steps.

4.3 Late record: use in the final ten minutes

Use only your case's released record and support card. Copy exact displays or line observations and reported categories, then state the limit.

Your teacher opens this record in the final ten minutes. Log it on the last page of the worksheet, copying exactly what it supplies and stating what it does not.

Open your Case A late record and support card

Log E5 / Exhibit 011 exactly as supplied. Make one row for each Saturday time. Copy 412 mg/dL in its row and HI, not a guessed number, in the other. Record the supplied fasting-card label/range and the separate stated meter ceiling. State that fasting status and meter accuracy are not established. Do not diagnose or infer a cause of death.

Case A support: a meter memory

Copy each stored display with its stated day, time and unit. Keep HI as a nonnumeric flag. Do not replace it with 600 or another guessed reading. The supplied record explains HI using its stated meter ceiling. Copy the supplied reference card with its fasting label, meaning a reference context without recent food intake. Fasting status and meter accuracy are not established by this worksheet. The log does not establish a diagnosis or cause of death. FDA display explanation [S5], FDA fasting-context explanation [S6].

Glucose meter memory readout (SIMULATED)

E5 / Exhibit 011. Record type: numerical reading and nonnumeric instrument flag.

Reported dayReported timeExact displayCalendar date
Saturday11:02 AM412 mg/dLnot reported
Saturday9:47 PMHInot reported

Separate stated meter ceiling: 600 mg/dL. HI, meaning the reading was above the meter's 600 mg/dL ceiling, so the screen shows HI instead of a number (reported instrument explanation, not independently validated meter behavior).

Supplied reference card: fasting glucose about 70 to 100 mg/dL.

Fasting status of the record subject: not reported. Clinical interpretation: none supplied. Cause or event inference: none supplied.

Log fields (left blank for your log): Evidence/exhibit / Reported day/time / Exact display / Unit where supplied / Fasting reference-card label/range / Separate meter ceiling / Missing context and limit.

Help when you need it

Need help? Choose a starting point

Need a running start
Read the ABO typing rule and the serum definitions, use Sample P and finish A1–A2.
On track
Four observation rows are supplied in A and three in B, including references with well observations. All five reference summaries are still used for each stain. Missing exact types remain categorical.
Stuck? Get unstuck
Lost your place? Find the next unanswered task ID. Use R2 for the grid, R3 for comparisons, R4 for the collection-plan explanation, R5 for arithmetic and R6 for the late record. Stay in the same worksheet.
Push me further
Hypothetical practice for both cases: a stain has classroom type B and a reference has type O. Write the same/different comparison and one limit. Do not change your case records. Limit the statement to this feature comparison, not a person's actions.

Guided notes and the separate worked model are support, not extra submissions. Guided notes can support your work but are not another submission.

Restart where you stopped

Lost your place? Find the next unanswered task ID. Use R2 for the grid, R3 for comparisons, R4 for the collection-plan explanation, R5 for arithmetic and R6 for the late record. Stay in the same worksheet.

If you were absent or need help

Use the same supplied-record handout and complete the same product. Its late-record section is included; work on that section after Parts A–E. You do not need to collect a new specimen or invent a missing observation. If your teacher separately assigns actual collection, follow the applicable preparation and approved instructions. This paper worksheet does not complete that practical requirement.

Lost your place? Find the next unanswered task ID and use F1's restart cue. Guided notes and the separate worked model are support, not extra submissions.

If your teacher is absent: Complete the supplied paper-record worksheet using the required briefing and models. Do not begin unassigned physical collection. Use the late-record section after Parts A–E.

Notebook support, not another submission

Keep notebook pages in your notebook. The model and these checks do not require another upload. Complete Parts A–E and your late-record log in the one serology worksheet.

Optional support
  • Optional Guided Notes
  • Separate Sample P and 150 worked models
  • Existing voluntary vocabulary extra credit, kept separate from required D1
Preparation gate for a separate lesson

Before any separate teacher-directed physical collection, complete the existing preparation requirement and use the approved instructions. This worksheet does not grant a practical pass.

Before any separate teacher-directed actual collection, complete the existing DNA Evidence from the Scene preparation requirement and use the approved instructions. The paper worksheet does not certify that practical work.

Connection to PLTW

Related PLTW reference:

  • Activity 1.1.4 Blood Evidence
  • Activity 1.1.5 DNA Evidence

This John Hay original worksheet practices model comparisons, careful record keeping and evidence limits connected to PLTW Activities 1.1.4 Blood Evidence and 1.1.5 DNA Evidence. It does not certify official activity completion. The complete required explanation is here; background links are support, not extra assignments.

John Hay original local work connected by title; no official completion claim.

Turn in one worksheet

Complete one serology worksheet, Parts A–E plus your case's late-record log. Hand the paper worksheet to Mr. Mendoza before leaving, or submit one PDF to this section's assignment by September 18 at 11:59 PM. Use your period and investigator code. Do not submit twice; keep notebook pages in your notebook.

The model and these checks do not require another upload. Complete Parts A–E and your late-record log in the one serology worksheet.

Case A: submit to Wk4 D09 · Whose Blood, Whose DNA (PLTW 1.1.4)

Keep today's worksheet for the next assigned case lesson. Later evidence must be supplied before you use it; do not assume a DNA or clinical result already exists.

Sources and optional background

F1 shows the paper-record workflow. The separate Sample P example models a comparison, and its 150 example models the arithmetic. Guided notes can support your work but are not another submission. Before any separate teacher-directed physical collection, complete the existing preparation requirement and use the approved instructions. This worksheet does not grant a practical pass. Carry your record to the next assigned case lesson, without assuming that it already has a DNA or clinical result.

A matching type does not identify a donor. No DNA profile is supplied for this worksheet, so do not make a DNA identification. The finished worksheet does not claim a test, collection, DNA identity or clinical conclusion.