mi Benchmark 1

Enter your Student ID and answer each item. This is a growth check against the WebXam blueprint, not a grade.

1. 1. The main purpose of PCR is to:

   AMake many copies of a target DNA regionBCut DNA at specific sitesCRead a protein sequenceDKill bacteria

2. 2. On a DNA gel, the fragments that travel farthest are the:

   ASmallest fragmentsBLargest fragmentsCMost charged proteinsDHeaviest cells

3. 3. A BLAST hit with an E-value of 1e-50 versus one of 5.0 is:

   AA much stronger match (smaller E-value)BA weaker matchCExactly the sameDNot comparable

4. 4. An ELISA that calls a healthy person positive has produced a:

   AFalse positiveBFalse negativeCTrue positiveDTrue negative

5. 5. A restriction enzyme is used to:

   ACut DNA at a specific recognition sequenceBJoin DNA fragmentsCCopy DNADStain DNA

6. 6. A bright spot on a gene-expression microarray means that gene is:

   AHighly expressed in the sampleBDeletedCA proteinDUnrelated to expression

7. 7. In gene therapy, a viral vector is used to:

   ADeliver a working gene into cellsBGive the patient a diseaseCRemove all DNADSequence the genome

8. 8. A key safety concern with CRISPR editing is:

   AOff-target edits at unintended sitesBIt cannot cut DNACIt only works in plantsDIt adds antibiotics

9. 9. A SNP is a:

   ASingle-base difference at one positionBMissing chromosomeCProteinDAntibody

10. 10. To make human insulin in bacteria, you first:

    AInsert the human insulin gene into a plasmidBInject bacteria into the patientCRemove all bacterial DNADSequence the patient

11. 11. A tumor suppressor gene normally:

    ARestrains cell divisionBSpeeds uncontrolled divisionCMakes antibodiesDDigests food

12. 12. Leaving a culture plate uncovered on the bench most likely causes:

    AAirborne contaminationBFaster correct resultsCSterilizationDNo change

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