Notebook submit
Mon, May 10, 2027 · Week 17 · Biotechnology for Health (Biomedical Innovations)
Today's goal: Assemble and submit a complete transformation and gel laboratory notebook.
This is a model of the work you should turn in today. Use it to check your own: match the structure and the level of detail, do not copy it. Your data and wording should be your own.
Notebook contents:
- Methods: heat-shock transformation, plating on four conditions, restriction digest, and agarose gel with a ladder.
- Plate data: 84 colonies on the plasmid + antibiotic plate; 0 colonies on the no-plasmid + antibiotic plate; lawns on both plain-agar plates.
- Gel data: standard curve from the ladder; sample bands estimated at about 600 base pairs and 380 base pairs.
Conclusion: The transformation worked, because colonies grew only where the plasmid and antibiotic were both present, and the negative control showed no growth. The gel fragment sizes are close to the predicted restriction map, so the plasmid identity is confirmed within estimation limits.
One source of error: My ladder bands were slightly smeared, which makes the migration distances I measured a little uncertain.
Future improvement: Next time I would load less DNA and run the gel a bit longer at a steady voltage to get sharper, easier-to-measure bands.
Status: Submitted to Schoology and confirmed in my tracker.
This model shows the level of evidence and organization needed to complete: Completes the assembled lab notebook: transformation notes, plate colony counts with units, the gel standard curve and fragment-size estimates with units, a conclusion on plasmid identity, one error source, and one future improvement.
- State the question and method.
- Present the observations and data with units.
- Explain the result, limitations, and next investigation.
Keep the structure. Replace the question, facts, measurements, and evidence. Then recheck units, vocabulary, and whether the conclusion goes beyond the evidence.
Also due today: Submit the finalized lab notebook on Schoology and confirm in your tracker.
Turn in: Complete transformation and gel lab notebook
Go to Schoology to turn this in.
Submit one PDF. Put your first and last name in the document header. Name the file: FirstName LastName - Assignment Title - YYYY-MM-DD.pdf.
Open Schoology PDF upload helpIf you cannot get in, see Mr. Mendoza. Do not skip the work.
Claim ceiling: Today's evidence supports a classroom claim about notebook submit. It cannot prove causation, diagnose a real patient, or justify action outside this room.
One exam-style question that uses exactly what you practiced today. Try it before you reveal the answer, then read why each choice is right or wrong.
Tap an answer to see the full explanation. Nothing is recorded or graded.
It builds this reusable test skill: Recording quantitative lab data with correct units.
- Name the concept or data pattern being tested.
- Cross out choices that violate that rule or the evidence.
- Justify the best remaining choice before checking the answer.