Notebook submit
Mon, May 3, 2027 · Week 16 · Biotechnology for Health (Biomedical Innovations)
Today's goal: Assemble and submit a complete transformation and gel laboratory notebook.
What a finished product looks like
This is a model of the work you should turn in today. Use it to check your own: match the structure and the level of detail, do not copy it. Your data and wording should be your own.
Notebook contents:
- Methods: heat-shock transformation, plating on four conditions, restriction digest, and agarose gel with a ladder.
- Plate data: 84 colonies on the plasmid + antibiotic plate; 0 colonies on the no-plasmid + antibiotic plate; lawns on both plain-agar plates.
- Gel data: standard curve from the ladder; sample bands estimated at about 600 base pairs and 380 base pairs.
Conclusion: The transformation worked, because colonies grew only where the plasmid and antibiotic were both present, and the negative control showed no growth. The gel fragment sizes are close to the predicted restriction map, so the plasmid identity is confirmed within estimation limits.
One source of error: My ladder bands were slightly smeared, which makes the migration distances I measured a little uncertain.
Future improvement: Next time I would load less DNA and run the gel a bit longer at a steady voltage to get sharper, easier-to-measure bands.
Status: Submitted to the LMS and confirmed in my tracker.
Also due today: Submit the finalized lab notebook in the course LMS and confirm in your tracker.
WebXam problem for today's skill
One exam-style question that uses exactly what you practiced today. Try it before you reveal the answer, then read why each choice is right or wrong.
Tap an answer to see the full explanation. Nothing is recorded or graded.