Gel map
Fri, Apr 30, 2027 · Week 15 · Biotechnology for Health (Biomedical Innovations)
Today's goal: Interpret your gel by estimating fragment sizes and confirming the plasmid identity.
What a finished product looks like
This is a model of the work you should turn in today. Use it to check your own: match the structure and the level of detail, do not copy it. Your data and wording should be your own.
Step 1, ladder distances (mm migrated from well):
- 1000 bp band: 14 mm
- 700 bp band: 19 mm
- 500 bp band: 24 mm
- 300 bp band: 31 mm
Step 2, standard curve: I plotted log(fragment size) on the y-axis against migration distance on the x-axis; the points fall on a near-straight line, so I can read sizes between the markers.
Step 3, sample band estimates from the curve:
- Sample band at 22 mm is about 600 bp.
- Sample band at 28 mm is about 380 bp.
Step 4, compare to the predicted restriction map: My map predicted a double digest would give fragments of about 600 bp and 400 bp.
Conclusion: My estimated sizes (about 600 bp and 380 bp) are close to the predicted 600 bp and 400 bp, so the gel is consistent with the expected construct and the plasmid identity is confirmed within the limits of gel estimation.
| Ladder band (bp) | Distance (mm) |
|---|---|
| 1000 | 14 |
| 700 | 19 |
| 500 | 24 |
| 300 | 31 |
Also due today: Submit your gel map data table and conclusion in the course LMS today.
WebXam problem for today's skill
One exam-style question that uses exactly what you practiced today. Try it before you reveal the answer, then read why each choice is right or wrong.
| Ladder band (bp) | Distance (mm) |
|---|---|
| 700 | 19 |
| 500 | 24 |
| Unknown | 22 |
Tap an answer to see the full explanation. Nothing is recorded or graded.