Mon, Apr 26, 2027Spring (Semester 2) · Week 15Day 62 of 6780-min block

Notebook submit

Today's target

Assemble and submit a complete transformation and gel laboratory notebook.

Due today · Lab report Required

Complete transformation and gel laboratory notebook: transformation notes, plate colony counts with units, gel standard curve, fragment-size estimates with units, conclusion on plasmid identity, one error source, and one future improvement.

Your 4 steps today
  1. 1
    Do this
    Assemble and submit a complete transformation and gel laboratory notebook.
  2. 2
  3. 3
    Submit this
    Lab report: Complete transformation and gel laboratory notebook: transformation notes, plate colony counts with units, gel standard curve, fragment-size estimates with units, conclusion on plasmid identity, one error source, and one future improvement.
  4. 4
    Submit it here
    1. 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
    2. 2Clever. Clever opens. Sign in if it asks.
    3. 3Microsoft (district) login. Use your district Microsoft account (the one for school).
    4. 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
    The file to submit is named: Biotechnology for Health (Biomedical Innovations) › Transformation, antibiotic selection, plasmid extraction, restriction digest, gel interpretation. › Lab report
    Open Schoology
Were you absent? Jump to the make-up plan
Where this fits
Tested on (Ohio WebXam)
Biotechnology for Health and Disease · 072125
PLTW lesson
BI · Notebook submit
WebXam domain
Microbiology Testing and Technology
Evidence to produce
Lab report
Lab / skill
Learn.Genetics (University of Utah): gel electrophoresis
Quick glossary
CER:
Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
SOP:
Standard Operating Procedure — the exact steps to follow (especially in a lab).
Tracker:
Your PLTW progress log where you record completed evidence.
myPLTW:
The PLTW course site where you do the online activities — you open it through Schoology.
Learn first

Minute-by-minute · 80-minute block

💡 Big idea: A complete laboratory notebook is the permanent record of experimental method, data, and conclusion.

  1. 0-5 minWarm-up: what is missing from a lab notebook that just has data but no conclusion?
  2. 5-25 minCompile transformation notes, plate colony counts, and gel map into one notebook document
  3. 25-45 minVerify all data has units; verify conclusion references both transformation and plasmid results
  4. 45-60 minAdd one error source and one specific future improvement
  5. 60-72 minFinal check: is every page dated and labeled?
  6. 72-80 minSubmit notebook and confirm in tracker
Mr. Mendoza's 5-minute intro
  • Today we close out the transformation-gel unit with a complete, submission-ready lab notebook.
  • The notebook is your permanent record: someone should be able to replicate your work from it.
  • Colony counts with units, fragment sizes with units, a conclusion, and one error: those are the requirements.
  • When you submit today, Problem 6 lab documentation is done.
Do this, step by step
  1. 1Compile your transformation notes, plate data, and gel map.
  2. 2Record colony counts and fragment-size estimates with units.
  3. 3State your conclusion about transformation and plasmid identity.
  4. 4Note one source of error and a future improvement.
  5. 5Submit the notebook and confirm it in your tracker.
You'll be able to
  • Your notebook documents transformation results and gel analysis.
  • It includes a conclusion and is submitted.
Know by the end
  • All numerical data in the notebook must include units.
  • A conclusion ties the data back to the original hypothesis or expected result.
  • Identifying one error source and an improvement shows scientific self-evaluation.
Do the work

Your PLTW work today

Open this PLTW section today

Transformation, antibiotic selection, plasmid extraction, restriction digest, gel interpretation. · Notebook submit

Day 5 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.

Do this: Open Problem 6 in your myPLTW course shell and confirm Problem 6 activities are complete, then assemble and submit your complete transformation and gel laboratory notebook.

Complete

Submit your finalized lab notebook to the Problem 6 portfolio.

How far to get

All Problem 6 milestones (workflow notes, transformation, gel analysis) should be complete by today.

Upload as evidence

Screenshot of your LMS submission confirmation as final evidence.

All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.

The plan

Today's PLTW tracker

Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.

Use the code Mr. Mendoza gave you, not your name. Saved on this device.

Transformation, antibiotic selection, plasmid extraction, restriction digest, gel interpretation.Day 5 of this projectSee the full week plan
Today's PLTW target

Transformation, antibiotic selection, plasmid extraction, restriction digest, gel interpretation. · Notebook submit

Open Problem 6 in your myPLTW course shell and confirm Problem 6 activities are complete, then assemble and submit your complete transformation and gel laboratory notebook.

All Problem 6 milestones (workflow notes, transformation, gel analysis) should be complete by today.

This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.

1 · What you do today

🎯 Assemble and submit a complete transformation and gel laboratory notebook.

  • Compile your transformation notes, plate data, and gel map.
  • Record colony counts and fragment-size estimates with units.
  • State your conclusion about transformation and plasmid identity.
  • Note one source of error and a future improvement.
  • Submit the notebook and confirm it in your tracker.
2 · Turn in today

Lab report: Complete transformation and gel laboratory notebook: transformation notes, plate colony counts with units, gel standard curve, fragment-size estimates with units, conclusion on plasmid identity, one error source, and one future improvement.

Submit on Schoology

Upload by 11:29 PM for full credit.

3 · Who's doing what (team)
TaskWho
Compile your transformation notes, plate data, and gel map._______
Record colony counts and fragment-size estimates with units._______
State your conclusion about transformation and plasmid identity._______
Note one source of error and a future improvement._______
Submit the notebook and confirm it in your tracker._______

Working solo? Put your own name in "Who" for every row.

4 · Words I can use correctly
5 · I'm successful today when I can…
  • Your notebook documents transformation results and gel analysis.
  • It includes a conclusion and is submitted.
6 · Reflection & next steps
Where are you today?0/7 checked
Pick your period and code first.
Explore

Teacher-posted resources

Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.

Catch-up / reteachFor: Need extra support
BI 6.1.2 Cloning Module 2 Transformation Overview
worksheet/handoutOpens here
Open the file

Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.

Placement rationale

Matched Transformation, gel electrophoresis, molecular evidence by path:Biomedical-Innovations/Problem-6_Molecular-Biology/6.1_Molecular-Biology; keywords:transformation, plasmid, molecular. Score 146. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

Catch-up / reteachFor: Need extra support
BI 6.1.2 Module I Restriction Enzyme Gel Results
worksheet/handoutOpens here
Open the file

Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.

Placement rationale

Matched Transformation, gel electrophoresis, molecular evidence by path:Biomedical-Innovations/Problem-6_Molecular-Biology/6.1_Molecular-Biology; keywords:gel, molecular. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

Catch-up / reteachFor: Need extra support
BI 6.1.2 Module II Control and Transformation Plates
worksheet/handoutOpens here
Open the file

Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.

Placement rationale

Matched Transformation, gel electrophoresis, molecular evidence by path:Biomedical-Innovations/Problem-6_Molecular-Biology/6.1_Molecular-Biology; keywords:transformation, molecular. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.

Lab day

Lab & supplies

Bring / set up
Provided transformation plate imagesGel electrophoresis chamber and power supplyAgarose gelDNA ladder standardRestriction digest samplesMicropipettes and tipsGel staining and imaging setup
Learn.Genetics (University of Utah): gel electrophoresis
Words

This unit's vocabulary

transformation/trans-for-MAY-shun/selectioncolonydigestgel electrophoresisDNA ladder

Tap the speaker to hear a term. Weekly vocabulary task: add two of these terms to your notebook glossary with a definition and an example in your own words.

Check yourself

WebXam practice

Tap an answer to check it · nothing is recorded or graded
To ensure preservation of incubated, refrigerated, and frozen reagents used in transformation and gel work, what must you closely monitor?
Before using an analytical balance to weigh agarose, a performance check shows the standard's mass reads too low. What is the next step?
What should you check to be sure a centrifuge used for a plasmid extraction is ready and safe to use?
After a restriction digest, you separate the DNA fragments on a gel. A reference lane of fragments of known sizes is included to estimate the sizes of your bands. This reference is the:
Check yourself

Cumulative WebXam review

A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.

Tap an answer to check it · nothing is recorded or graded
[Review: Investigating an Outbreak: line lists, incidence, and intervention design] Which pair of terms correctly describes the difference between morbidity and mortality?
[Review: Communicating Public Health: audience, privacy, and evidence-based products] Usability testing of a health education website shows that users cannot find the main instructions. What should the team do?
[Review: Recombinant DNA Workflow: cutting, joining, and moving genes safely] In which storage cabinet should you keep the rubbing (isopropyl) alcohol used to sterilize a molecular biology bench?
To ensure preservation of incubated, refrigerated, and frozen reagents used in transformation and gel work, what must you closely monitor?
Explore

Where this leads — careers

Safety net

What to do if you were absent

If YOU are absent

Today is individual PLTW work, so do exactly what we did in class, from home: complete the same PLTW target above, then submit your Lab report.

Open Schoology (CMSD) and keep going

How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.

If MR. MENDOZA is absent

Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:

Learn.Genetics (University of Utah): gel electrophoresis
Explore

Optional extra credit (async)

You've passed Unit 2, so the optional extra-credit track is open. Complete reserved-unit work from home (virtual labs included) for extra credit, all submitted on Schoology.

Open the extra-credit track
How this is graded
For: Lab report — Complete transformation and gel laboratory notebook: transformation notes, plate colony counts with units, gel standard curve, fragment-size estimates with units, conclusion on plasmid identity, one error source, and one future improvement.
  • Complete
    Every required part of the artifact is present, nothing left blank.
  • Accurate
    The science and the data are correct and match the evidence.
  • Scientific reasoning
    You explain your claim with evidence and reasoning (CER), not just an answer.
  • Professional communication
    Clear, organized, labeled, and written the way a clinician or scientist would.
  • Submitted
    Turned in the right way (Schoology for routine work) and confirmed.
Submission Zone

Drop your Mon, Apr 26, 2027 · Notebook submit here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).

Upload a project