Gel map
Interpret your gel by estimating fragment sizes and confirming the plasmid identity.
Gel analysis table with ladder band migration distances, standard curve (log fragment size vs. distance), estimated sample fragment sizes, predicted restriction map sizes, and a written conclusion on plasmid identity.
- 1Do thisInterpret your gel by estimating fragment sizes and confirming the plasmid identity.
- 2Use this resource
- 3Submit thisData table: Gel analysis table with ladder band migration distances, standard curve (log fragment size vs. distance), estimated sample fragment sizes, predicted restriction map sizes, and a written conclusion on plasmid identity.
- 4Submit it here
- 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
- 2Clever. Clever opens. Sign in if it asks.
- 3Microsoft (district) login. Use your district Microsoft account (the one for school).
- 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
The file to submit is named: Biotechnology for Health (Biomedical Innovations) › Transformation, antibiotic selection, plasmid extraction, restriction digest, gel interpretation. › Data tableOpen Schoology
- CER:
- Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure — the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities — you open it through Schoology.
Minute-by-minute · 80-minute block
💡 Big idea: A gel map converts band position into fragment size and confirms whether a construct is correct.
- 0-5 minWarm-up: why does a smaller DNA fragment migrate farther in an agarose gel?
- 5-20 minMeasure migration distances for every ladder band and record in a table
- 20-40 minBuild a standard curve (log fragment size vs. migration distance)
- 40-55 minInterpolate estimated sizes for all sample bands using the curve
- 55-70 minCompare estimates to the predicted restriction map; write your conclusion
- 70-80 minExit ticket: state whether your plasmid matches the expected construct and why
- • Yesterday you ran the gel. Today you extract the information from it.
- • The ladder is the key: each known band gives you one point on a standard curve.
- • Once you have the curve, you read off the size of every sample band.
- • If your sizes match the predicted restriction map, you've confirmed the plasmid.
- 1Measure the migration distance of the DNA ladder bands.
- 2Build a standard curve relating distance to fragment size.
- 3Estimate the size of each sample band from the curve.
- 4Compare estimated sizes to the expected restriction map.
- 5Conclude whether the plasmid matches the predicted construct.
- • You estimated sample fragment sizes from a ladder.
- • You concluded whether the gel matches the expected map.
- • Migration distance is inversely related to fragment size on an agarose gel.
- • A log-linear standard curve built from the ladder lets you interpolate unknown fragment sizes.
- • If estimated fragment sizes match the predicted restriction map, the plasmid identity is confirmed.
Your PLTW work today
Transformation, antibiotic selection, plasmid extraction, restriction digest, gel interpretation. · Gel map
Day 4 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.
Do this: Open Problem 6 in your myPLTW course shell and navigate to the gel analysis activity, then interpret your gel by estimating fragment sizes from the DNA ladder.
Add your standard curve and fragment-size estimates to the Problem 6 portfolio.
The wet lab data is collected; gel analysis is the interpretation milestone following the lab, so check your activity guide.
Standard curve plot and comparison table submitted as evidence.
All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.
Today's PLTW tracker
Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.
Use the code Mr. Mendoza gave you, not your name. Saved on this device.
Transformation, antibiotic selection, plasmid extraction, restriction digest, gel interpretation. · Gel map
Open Problem 6 in your myPLTW course shell and navigate to the gel analysis activity, then interpret your gel by estimating fragment sizes from the DNA ladder.
The wet lab data is collected; gel analysis is the interpretation milestone following the lab, so check your activity guide.
This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.
🎯 Interpret your gel by estimating fragment sizes and confirming the plasmid identity.
- Measure the migration distance of the DNA ladder bands.
- Build a standard curve relating distance to fragment size.
- Estimate the size of each sample band from the curve.
- Compare estimated sizes to the expected restriction map.
- Conclude whether the plasmid matches the predicted construct.
Data table: Gel analysis table with ladder band migration distances, standard curve (log fragment size vs. distance), estimated sample fragment sizes, predicted restriction map sizes, and a written conclusion on plasmid identity.
Submit on SchoologyUpload by 11:29 PM for full credit.
| Task | Who |
|---|---|
| Measure the migration distance of the DNA ladder bands. | _______ |
| Build a standard curve relating distance to fragment size. | _______ |
| Estimate the size of each sample band from the curve. | _______ |
| Compare estimated sizes to the expected restriction map. | _______ |
| Conclude whether the plasmid matches the predicted construct. | _______ |
Working solo? Put your own name in "Who" for every row.
- You estimated sample fragment sizes from a ladder.
- You concluded whether the gel matches the expected map.
Teacher-posted resources
Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched Transformation, gel electrophoresis, molecular evidence by path:Biomedical-Innovations/Problem-6_Molecular-Biology/6.1_Molecular-Biology; keywords:transformation, plasmid, molecular. Score 146. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched Transformation, gel electrophoresis, molecular evidence by path:Biomedical-Innovations/Problem-6_Molecular-Biology/6.1_Molecular-Biology; keywords:gel, molecular. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched Transformation, gel electrophoresis, molecular evidence by path:Biomedical-Innovations/Problem-6_Molecular-Biology/6.1_Molecular-Biology; keywords:transformation, molecular. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.
Lab & supplies
WebXam practice
Cumulative WebXam review
A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.
Where this leads — careers
What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.
What to do if you were absent
Today is individual PLTW work, so do exactly what we did in class, from home: complete the same PLTW target above, then submit your Data table.
Open Schoology (CMSD) and keep goingHow to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.
Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:
Learn.Genetics (University of Utah): gel electrophoresisOptional extra credit (async)
You've passed Unit 2, so the optional extra-credit track is open. Complete reserved-unit work from home (virtual labs included) for extra credit, all submitted on Schoology.
Open the extra-credit track- CompleteEvery required part of the artifact is present, nothing left blank.
- AccurateThe science and the data are correct and match the evidence.
- Scientific reasoningYou explain your claim with evidence and reasoning (CER), not just an answer.
- Professional communicationClear, organized, labeled, and written the way a clinician or scientist would.
- SubmittedTurned in the right way (Schoology for routine work) and confirmed.
Drop your Fri, Apr 23, 2027 · Gel map here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).
Upload a project