Workflow notes and controls
Outline the recombinant DNA workflow and explain the rationale for each control.
Recombinant DNA workflow outline with four ordered steps, named enzyme or reagent for each, restriction enzyme specificity explanation, and positive/negative control identification with safety rationale.
- 1Do thisOutline the recombinant DNA workflow and explain the rationale for each control.
- 2Use this resource
- 3Submit thisPre-lab: Recombinant DNA workflow outline with four ordered steps, named enzyme or reagent for each, restriction enzyme specificity explanation, and positive/negative control identification with safety rationale.
- 4Submit it here
- 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
- 2Clever. Clever opens. Sign in if it asks.
- 3Microsoft (district) login. Use your district Microsoft account (the one for school).
- 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
The file to submit is named: Biotechnology for Health (Biomedical Innovations) › Recombinant DNA workflow, restriction enzymes, ligation, transformation safety. › Pre-labOpen Schoology
- CER:
- Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure — the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities — you open it through Schoology.
Minute-by-minute · 80-minute block
💡 Big idea: Every step in the recombinant DNA workflow has a specific enzyme, a specific purpose, and controls that verify the outcome.
- 0-5 minWarm-up: what does a restriction enzyme actually do to DNA?
- 5-20 minList workflow steps and name the enzyme or reagent for each
- 20-40 minExplain restriction enzyme specificity; sketch a sticky-end diagram
- 40-55 minIdentify positive and negative controls; write safety rationale for each
- 55-70 minPartner check: can your partner identify all four steps and both controls?
- 70-80 minExit ticket: name the enzyme for each workflow step and both controls
- • Today we map the four-step molecular workflow you'll run in a future lab.
- • Cut, ligate, transform, select: each step has a specific molecule doing a specific job.
- • We'll also nail down why controls are not optional: they're how you know your result is real.
- • By the end you'll have a complete workflow reference and a control rationale you can defend.
- 1List the workflow steps: cut, ligate, transform, select.
- 2For each step, name the enzyme or reagent used.
- 3Explain why restriction enzymes cut at specific sequences.
- 4Identify a positive and a negative control for the experiment.
- 5Write the safety reason each control is included.
- • You ordered the recombinant DNA workflow correctly.
- • You justified a positive and a negative control.
- • Restriction endonucleases recognize and cut DNA at palindromic sequences, generating compatible sticky ends.
- • DNA ligase seals the nicks between the insert and vector after annealing.
- • Antibiotic selection plates distinguish transformed cells from non-transformed cells.
Your PLTW work today
Recombinant DNA workflow, restriction enzymes, ligation, transformation safety. · Workflow notes and controls
Day 2 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.
Do this: Open Problem 6 in your myPLTW course shell and navigate to the current activity, then outline the recombinant DNA workflow and explain the rationale for each control.
Attach your workflow notes and control rationale to the Problem 6 evidence portfolio.
The ethics debate is done; workflow notes are an early Problem 6 milestone, so check your activity guide and submit today.
Completed workflow notes with enzyme names and control rationale submitted.
All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.
Today's PLTW tracker
Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.
Use the code Mr. Mendoza gave you, not your name. Saved on this device.
Recombinant DNA workflow, restriction enzymes, ligation, transformation safety. · Workflow notes and controls
Open Problem 6 in your myPLTW course shell and navigate to the current activity, then outline the recombinant DNA workflow and explain the rationale for each control.
The ethics debate is done; workflow notes are an early Problem 6 milestone, so check your activity guide and submit today.
This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.
🎯 Outline the recombinant DNA workflow and explain the rationale for each control.
- List the workflow steps: cut, ligate, transform, select.
- For each step, name the enzyme or reagent used.
- Explain why restriction enzymes cut at specific sequences.
- Identify a positive and a negative control for the experiment.
- Write the safety reason each control is included.
Pre-lab: Recombinant DNA workflow outline with four ordered steps, named enzyme or reagent for each, restriction enzyme specificity explanation, and positive/negative control identification with safety rationale.
Submit on SchoologyUpload by 11:29 PM for full credit.
| Task | Who |
|---|---|
| List the workflow steps: cut, ligate, transform, select. | _______ |
| For each step, name the enzyme or reagent used. | _______ |
| Explain why restriction enzymes cut at specific sequences. | _______ |
| Identify a positive and a negative control for the experiment. | _______ |
| Write the safety reason each control is included. | _______ |
Working solo? Put your own name in "Who" for every row.
- You ordered the recombinant DNA workflow correctly.
- You justified a positive and a negative control.
Teacher-posted resources
Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched Molecular biology workflow and cloning by path:Biomedical-Innovations/Problem-6_Molecular-Biology/00_Problem-Overview; keywords:molecular biology, recombinant dna. Score 146. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched Molecular biology workflow and cloning by path:Biomedical-Innovations/Problem-6_Molecular-Biology/6.1_Molecular-Biology; keywords:recombinant dna, cloning. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched Molecular biology workflow and cloning by path:Biomedical-Innovations/Problem-6_Molecular-Biology/6.1_Molecular-Biology; keywords:recombinant dna, cloning. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.
Lab & supplies
- • No wet chemicals today; this is a notes and diagram session.
- • If using physical model components, handle small pieces carefully to avoid choking hazard for younger students in adjacent rooms.
- • Keep workspace organized; loose paper fragments from cut-out diagrams should be collected before leaving.
WebXam practice
Cumulative WebXam review
A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.
Where this leads — careers
What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.
What to do if you were absent
Complete a virtual cloning workflow: select a restriction enzyme, cut and ligate the plasmid on screen, and record the predicted recombinant product.
Learn.Genetics virtual labsThen submit your Pre-lab on Schoology.
Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:
Learn.Genetics (University of Utah): cloning and recombinant DNAOptional extra credit (async)
You've passed Unit 2, so the optional extra-credit track is open. Complete reserved-unit work from home (virtual labs included) for extra credit, all submitted on Schoology.
Open the extra-credit track- CompleteEvery required part of the artifact is present, nothing left blank.
- AccurateThe science and the data are correct and match the evidence.
- Scientific reasoningYou explain your claim with evidence and reasoning (CER), not just an answer.
- Professional communicationClear, organized, labeled, and written the way a clinician or scientist would.
- SubmittedTurned in the right way (Schoology for routine work) and confirmed.
Drop your Mon, Apr 19, 2027 · Workflow notes and controls here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).
Upload a project