Biomolecule and tox data
Run biomolecule indicator tests following an SOP and collect data on unknown samples.
Indicator-test data table: columns for sample ID, each of the four indicators, color result, and interpretation (positive/negative); plus dilution series data table with concentration and observed effect.
- 1Do thisRun biomolecule indicator tests following an SOP and collect data on unknown samples.
- 2Use this resource
- 3Submit thisData table: Indicator-test data table: columns for sample ID, each of the four indicators, color result, and interpretation (positive/negative); plus dilution series data table with concentration and observed effect.
- 4Submit it here
- 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
- 2Clever. Clever opens. Sign in if it asks.
- 3Microsoft (district) login. Use your district Microsoft account (the one for school).
- 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
The file to submit is named: Principles of Biomedical Technology (Principles of Biomedical Science) › Unit 1.1 to 1.2: Experimental design in evidence testing; transition to autopsy evidence and biomolecules. › Data tableOpen Schoology
- CER:
- Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure — the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities — you open it through Schoology.
Minute-by-minute · 80-minute block
💡 Big idea: Controlled indicator tests produce valid forensic evidence only when positive and negative controls confirm the reagents are working correctly.
- 0:00Review SOP as a class; assign unknowns; review PPE requirements for today's reagents
- 0:10Set up labeled tube rack: positive control, negative control, and unknowns A-D
- 0:18Run indicator tests in order (sugars, starch, protein, lipids); record color results immediately
- 0:45Set up and record toxicology dilution series; note concentration as independent variable
- 1:00Record one contamination risk and one limitation in lab notebook
- 1:10Clean up stations per SOP disposal instructions; preview Thursday analysis
- • Today is a hands-on chemistry day. You will be testing unknown samples with indicator reagents. These reagents are not dangerous if handled properly, but they will stain your clothes and skin, so follow the PPE protocol exactly.
- • The most important word today is control. A positive control tells us the reagent is working. A negative control tells us a color change is not just contamination. Without both, your data means nothing.
- • You will also set up a toxicology dilution series. We are going to change one variable, the concentration, and record the effect. This is the foundation of every toxicology study in medicine.
- • Read the SOP before you touch anything. Label every tube before you add anything to it. Record results immediately; do not wait until the end of the lab.
- 1Read the indicator-test SOP and set up labeled tubes with controls.
- 2Test each unknown for sugars, starch, protein, and lipids per the SOP.
- 3Record color-change results in a data table with positive and negative controls.
- 4Note the concentration variable in the toxicology dilution series.
- 5Record one limitation and one contamination risk in your method.
- • I can run indicator tests with proper controls.
- • I can record results in a controlled data table.
- • Every indicator test requires a positive control (known positive sample) and a negative control (distilled water or known negative) run alongside unknowns.
- • Cross-contamination between tubes produces false positives; use a clean dropper or pipette for every transfer.
- • A dilution series changes one variable (concentration) while holding all others constant, allowing a dose-response relationship to be observed.
Your PLTW work today
Unit 1.1 to 1.2: Experimental design in evidence testing; transition to autopsy evidence and biomolecules. · Biomolecule and tox data
Day 3 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.
Do this: In myPLTW, open the Lesson 1.1 Investigating the Scene evidence-testing lab section and record your team's unknown sample ID and assigned tests.
Mark the Lesson 1.1 lab data-entry task started in myPLTW and record your raw indicator-test data.
You prepared your hypothesis and design Tuesday. Today all four indicator tests and the dilution series should be complete with data recorded before cleanup.
Completed data table (all four indicator tests with controls and dilution series results) submitted through the class tracker or photographed into myPLTW.
All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.
Today's PLTW tracker
Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.
Use the code Mr. Mendoza gave you, not your name. Saved on this device.
Unit 1.1 to 1.2: Experimental design in evidence testing; transition to autopsy evidence and biomolecules. · Biomolecule and tox data
In myPLTW, open the Lesson 1.1 Investigating the Scene evidence-testing lab section and record your team's unknown sample ID and assigned tests.
You prepared your hypothesis and design Tuesday. Today all four indicator tests and the dilution series should be complete with data recorded before cleanup.
This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.
🎯 Run biomolecule indicator tests following an SOP and collect data on unknown samples.
- Read the indicator-test SOP and set up labeled tubes with controls.
- Test each unknown for sugars, starch, protein, and lipids per the SOP.
- Record color-change results in a data table with positive and negative controls.
- Note the concentration variable in the toxicology dilution series.
- Record one limitation and one contamination risk in your method.
Data table: Indicator-test data table: columns for sample ID, each of the four indicators, color result, and interpretation (positive/negative); plus dilution series data table with concentration and observed effect.
Submit on SchoologyUpload by 11:29 PM for full credit.
| Task | Who |
|---|---|
| Read the indicator-test SOP and set up labeled tubes with controls. | _______ |
| Test each unknown for sugars, starch, protein, and lipids per the SOP. | _______ |
| Record color-change results in a data table with positive and negative controls. | _______ |
| Note the concentration variable in the toxicology dilution series. | _______ |
| Record one limitation and one contamination risk in your method. | _______ |
Working solo? Put your own name in "Who" for every row.
- I can run indicator tests with proper controls.
- I can record results in a controlled data table.
Resources & readings
Hand-picked materials for this lesson. Class file items open the document directly; the rest are vetted readings and interactives from other biomedical programs.
Lab & supplies
- • Lugol's iodine is a stain: wear nitrile gloves and a lab apron; it will permanently stain skin and clothing.
- • Benedict's solution requires heating: use a hot-water bath, not an open flame; use tongs when handling hot tubes; never point a heated tube at anyone.
- • Biuret reagent contains sodium hydroxide, which is corrosive: avoid skin and eye contact; if contact occurs, flush immediately with water for 15 minutes and notify the teacher.
- • Sudan IV is a potential carcinogen: minimize skin contact, work in a well-ventilated area, and dispose of Sudan IV waste in the labeled hazardous-waste container, not the sink.
- • All liquid reagent waste goes into the designated waste beaker for disposal per the teacher's SDS instructions; never pour indicators down the sink without authorization.
- • Wash hands with soap and water for at least 20 seconds after completing all lab work, even if gloves were worn throughout.
WebXam practice
Cumulative WebXam review
A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.
Where this leads — careers
What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.
What to do if you were absent
Complete the virtual biomolecule indicator lab, run a dilution series, and record a data table with controls and one stated limitation.
PhET ConcentrationThen submit your Data table on Schoology.
Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:
Khan Academy: macromolecules- CompleteEvery required part of the artifact is present, nothing left blank.
- AccurateThe science and the data are correct and match the evidence.
- Scientific reasoningYou explain your claim with evidence and reasoning (CER), not just an answer.
- Professional communicationClear, organized, labeled, and written the way a clinician or scientist would.
- SubmittedTurned in the right way (Schoology for routine work) and confirmed.
Drop your Thu, Sep 10, 2026 · Biomolecule and tox data here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).
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