GFP and chromatography
Explain how GFP and chromatography let you track and separate a target protein.
Labeled chromatography diagram showing protein binding, wash, elution, fraction collection, and GFP signal prediction.
- 1Do thisExplain how GFP and chromatography let you track and separate a target protein.
- 2Use this resource
- 3Submit thisNotebook check: Labeled chromatography diagram showing protein binding, wash, elution, fraction collection, and GFP signal prediction.
- 4Submit it here
- 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
- 2Clever. Clever opens. Sign in if it asks.
- 3Microsoft (district) login. Use your district Microsoft account (the one for school).
- 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
The file to submit is named: Genetics of Disease (Medical Interventions) › GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. › Notebook checkOpen Schoology
- CER:
- Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure — the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities — you open it through Schoology.
Minute-by-minute · 80-minute block
💡 Big idea: GFP is a visual reporter that makes an invisible protein visible; chromatography exploits binding affinity to isolate it.
- 0-10Read chromatography notes; define chromatography and elution
- 10-25Explain GFP reporter mechanism; annotate UV signal
- 25-45Diagram protein binding to column resin and wash steps
- 45-58Add elution step to diagram; label target fraction
- 58-70Predict which numbered fraction glows; write prediction
- 70-80Submit labeled diagram to tracker; confirm lab readiness
- • Tomorrow you run the column, so today you need to understand what it is actually doing.
- • GFP is the tracking beacon: wherever it glows, your protein is.
- • Chromatography is the separation engine: it holds your protein while everything else washes away.
- • Understanding affinity and elution is a Lab SOPs and Molecular Technology skill on the WebXam.
- 1Read the chromatography notes in the PLTW course shell and define chromatography and elution.
- 2Explain why GFP glowing under UV light marks where the target protein is.
- 3Diagram a protein binding to a column and then eluting in a chosen fraction.
- 4Predict which fraction should glow if purification worked.
- 5Submit a labeled chromatography diagram as PLTW tracker evidence.
- • You'll be able to explain how GFP marks the target protein.
- • You'll be able to describe how chromatography separates and elutes proteins.
- • GFP fused to the target protein fluoresces green under UV light, revealing which fraction contains the protein.
- • Affinity chromatography uses a resin that binds the target specifically, letting other proteins wash through.
- • Elution uses a competing molecule or change in buffer conditions to release the bound target protein.
Your PLTW work today
GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. · GFP and chromatography
Day 2 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.
Do this: Open Activity 4.1.3 GFP Protein Purification in myPLTW and diagram how GFP and affinity chromatography track and separate the target protein.
Mark the GFP-and-chromatography entry complete and attach your labeled chromatography diagram.
Purification overview exit ticket should be done (Monday); chromatography diagram due today.
Labeled chromatography diagram with binding, wash, elution, and GFP fraction prediction submitted.
All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.
Today's PLTW tracker
Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.
Use the code Mr. Mendoza gave you, not your name. Saved on this device.
GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. · GFP and chromatography
Open Activity 4.1.3 GFP Protein Purification in myPLTW and diagram how GFP and affinity chromatography track and separate the target protein.
Purification overview exit ticket should be done (Monday); chromatography diagram due today.
This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.
🎯 Explain how GFP and chromatography let you track and separate a target protein.
- Read the chromatography notes in the PLTW course shell and define chromatography and elution.
- Explain why GFP glowing under UV light marks where the target protein is.
- Diagram a protein binding to a column and then eluting in a chosen fraction.
- Predict which fraction should glow if purification worked.
- Submit a labeled chromatography diagram as PLTW tracker evidence.
Notebook check: Labeled chromatography diagram showing protein binding, wash, elution, fraction collection, and GFP signal prediction.
Submit on SchoologyUpload by 11:29 PM for full credit.
| Task | Who |
|---|---|
| Read the chromatography notes in the PLTW course shell and define chromatography and elution. | _______ |
| Explain why GFP glowing under UV light marks where the target protein is. | _______ |
| Diagram a protein binding to a column and then eluting in a chosen fraction. | _______ |
| Predict which fraction should glow if purification worked. | _______ |
| Submit a labeled chromatography diagram as PLTW tracker evidence. | _______ |
Working solo? Put your own name in "Who" for every row.
- You'll be able to explain how GFP marks the target protein.
- You'll be able to describe how chromatography separates and elutes proteins.
Teacher-posted resources
Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched Protein purification and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:protein purification, gfp, chromatography. Score 150. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched Protein purification and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:protein purification, gfp, chromatography. Score 150. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched Protein purification and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:gfp, chromatography. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.
Lab & supplies
WebXam practice
Cumulative WebXam review
A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.
Where this leads — careers
What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.
What to do if you were absent
Today is individual PLTW work, so do exactly what we did in class, from home: complete the same PLTW target above, then submit your Notebook check.
Open Schoology (CMSD) and keep goingHow to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.
Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:
Genetic Science Learning Center: Genetics basics and proteinsOptional extra credit (async)
You've passed Unit 2, so the optional extra-credit track is open. Complete reserved-unit work from home (virtual labs included) for extra credit, all submitted on Schoology.
Open the extra-credit track- CompleteEvery required part of the artifact is present, nothing left blank.
- AccurateThe science and the data are correct and match the evidence.
- Scientific reasoningYou explain your claim with evidence and reasoning (CER), not just an answer.
- Professional communicationClear, organized, labeled, and written the way a clinician or scientist would.
- SubmittedTurned in the right way (Schoology for routine work) and confirmed.
Drop your Thu, Apr 29, 2027 · GFP and chromatography here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).
Upload a project