Protein-purification lab
Run a protein-purification procedure and collect fractions to isolate the target protein.
Fraction-collection data sheet recording tube number, buffer applied, GFP signal (yes/no), and target-fraction labels plus one error-control note.
- 1Do thisRun a protein-purification procedure and collect fractions to isolate the target protein.
- 2Use this resource
- 3Submit thisData table: Fraction-collection data sheet recording tube number, buffer applied, GFP signal (yes/no), and target-fraction labels plus one error-control note.
- 4Submit it here
- 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
- 2Clever. Clever opens. Sign in if it asks.
- 3Microsoft (district) login. Use your district Microsoft account (the one for school).
- 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
The file to submit is named: Genetics of Disease (Medical Interventions) › GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. › Data tableOpen Schoology
- CER:
- Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure — the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities — you open it through Schoology.
Minute-by-minute · 80-minute block
💡 Big idea: Column chromatography physically separates proteins by exploiting molecular differences in binding affinity.
- 0-10Read protocol; set up column and label collection tubes
- 10-25Load protein mixture; begin collecting fractions as wash proceeds
- 25-45Add elution buffer; collect elution fractions in order
- 45-58Check fractions under UV; record which fractions glow
- 58-70Label target fractions; note source of error and control
- 70-80Clean up column and bench; submit fraction-collection data sheet
- • Today is the protein-purification lab.
- • You will run a chromatography column, collect numbered fractions, and use UV light to find your protein.
- • Work precisely: fraction order matters, and mislabeling a tube loses data you cannot recover.
- • Fraction data collected today feeds directly into Thursday's gel interpretation.
- 1Read the lab protocol in the PLTW course shell and set up your column and tubes.
- 2Load the protein mixture and begin collecting numbered fractions.
- 3Add buffer to elute the bound protein and watch for the GFP signal under UV.
- 4Record which fractions glow and label them as your target collection.
- 5Note one source of error and how you controlled for it.
- 6Submit your fraction-collection data sheet.
- • You'll be able to run a column purification and collect fractions.
- • You'll be able to identify target fractions using the GFP signal.
- • Loading the lysate applies all proteins to the resin; only the target binds with high affinity.
- • Wash steps remove loosely bound contaminants before elution begins.
- • GFP fluorescence under UV is the qualitative signal that confirms the target protein is in a fraction.
Your PLTW work today
GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. · Protein-purification lab
Day 3 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.
Do this: Open Activity 4.1.3 GFP Protein Purification in myPLTW and run the protein-purification lab protocol to collect and identify your target fractions.
Mark the protein-purification lab entry complete and attach your fraction-collection data sheet.
Chromatography diagram should be done (Tuesday); fraction-collection data sheet due today.
Fraction-collection data sheet with tube numbers, buffer, GFP signal, and error-control note submitted.
All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.
Today's PLTW tracker
Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.
Use the code Mr. Mendoza gave you, not your name. Saved on this device.
GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. · Protein-purification lab
Open Activity 4.1.3 GFP Protein Purification in myPLTW and run the protein-purification lab protocol to collect and identify your target fractions.
Chromatography diagram should be done (Tuesday); fraction-collection data sheet due today.
This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.
🎯 Run a protein-purification procedure and collect fractions to isolate the target protein.
- Read the lab protocol in the PLTW course shell and set up your column and tubes.
- Load the protein mixture and begin collecting numbered fractions.
- Add buffer to elute the bound protein and watch for the GFP signal under UV.
- Record which fractions glow and label them as your target collection.
- Note one source of error and how you controlled for it.
- Submit your fraction-collection data sheet.
Data table: Fraction-collection data sheet recording tube number, buffer applied, GFP signal (yes/no), and target-fraction labels plus one error-control note.
Submit on SchoologyUpload by 11:29 PM for full credit.
| Task | Who |
|---|---|
| Read the lab protocol in the PLTW course shell and set up your column and tubes. | _______ |
| Load the protein mixture and begin collecting numbered fractions. | _______ |
| Add buffer to elute the bound protein and watch for the GFP signal under UV. | _______ |
| Record which fractions glow and label them as your target collection. | _______ |
| Note one source of error and how you controlled for it. | _______ |
| Submit your fraction-collection data sheet. | _______ |
Working solo? Put your own name in "Who" for every row.
- You'll be able to run a column purification and collect fractions.
- You'll be able to identify target fractions using the GFP signal.
Teacher-posted resources
Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched Protein purification and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:protein purification, gfp, chromatography. Score 150. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched Protein purification and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:protein purification, gfp, chromatography. Score 150. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched Protein purification and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:gfp, chromatography. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.
Lab & supplies
- • Wear nitrile gloves and safety goggles throughout the procedure.
- • UV light is harmful to eyes and skin; never look directly into the UV lamp and minimize exposure to skin.
- • Treat all protein samples as potential allergens; avoid contact with eyes and mouth.
- • Dispose of all biological waste in designated containers per lab protocol.
- • Wipe the bench with 70% ethanol before and after the procedure.
- • Report any spill or broken glassware to the teacher immediately.
WebXam practice
Cumulative WebXam review
A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.
Where this leads — careers
What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.
What to do if you were absent
Complete the virtual protein-purification run linked in the course shell, recording which fractions show the GFP signal under UV, then submit your fraction-collection data sheet.
Learn.Genetics: Gel ElectrophoresisThen submit your Data table on Schoology.
Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:
Genetic Science Learning Center: Genetics basics and proteinsOptional extra credit (async)
You've passed Unit 2, so the optional extra-credit track is open. Complete reserved-unit work from home (virtual labs included) for extra credit, all submitted on Schoology.
Open the extra-credit track- CompleteEvery required part of the artifact is present, nothing left blank.
- AccurateThe science and the data are correct and match the evidence.
- Scientific reasoningYou explain your claim with evidence and reasoning (CER), not just an answer.
- Professional communicationClear, organized, labeled, and written the way a clinician or scientist would.
- SubmittedTurned in the right way (Schoology for routine work) and confirmed.
Drop your Fri, Apr 30, 2027 · Protein-purification lab here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).
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