Purification overview
Explain why a manufactured protein must be purified and what purity means for a medicine.
Short exit ticket defining purity and identifying one concrete safety risk of an impure protein medicine.
- 1Do thisExplain why a manufactured protein must be purified and what purity means for a medicine.
- 2Use this resource
- 3Submit thisExit ticket: Short exit ticket defining purity and identifying one concrete safety risk of an impure protein medicine.
- 4Submit it here
- 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
- 2Clever. Clever opens. Sign in if it asks.
- 3Microsoft (district) login. Use your district Microsoft account (the one for school).
- 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
The file to submit is named: Genetics of Disease (Medical Interventions) › GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. › Exit ticketOpen Schoology
- CER:
- Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure — the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities — you open it through Schoology.
Minute-by-minute · 80-minute block
💡 Big idea: A protein medicine is only as useful as it is pure; contaminants can trigger immune reactions or reduce efficacy.
- 0-10Read purification overview; define purity in own words
- 10-25List cell-lysate components besides target protein
- 25-42Explain safety risk of each contaminant type
- 42-58Preview this week's purification steps (chromatography, SDS-PAGE)
- 58-70Write exit ticket: purity definition and one impurity risk
- 70-80Submit exit ticket; preview Tuesday chromatography diagram
- • Your bacteria made the recombinant protein, but it is swimming in a soup of thousands of other molecules.
- • Before it can enter a patient, everything else has to be removed.
- • Today you learn what is in that soup and why impurity is not just a quality problem but a safety problem.
- • Purity and QC connect to the Lab SOPs domain of the 072130 WebXam.
- 1Read the purification overview notes in the PLTW course shell and define purity.
- 2List what else is in the cell mixture besides the target protein.
- 3Explain why an impure protein medicine could be unsafe.
- 4Preview the purification steps you will model this week.
- 5Submit a short exit ticket defining purity and one risk of impurity.
- • You'll be able to explain why a protein medicine must be purified.
- • You'll be able to define purity and a risk of impurity.
- • Cell lysate contains the target protein mixed with thousands of other bacterial proteins, DNA, and membrane fragments.
- • Contaminants in a therapeutic protein can provoke dangerous immune responses in patients.
- • Purity is quantified by the ratio of target protein to total protein, often confirmed by gel analysis.
Your PLTW work today
GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. · Purification overview
Day 1 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.
Do this: Open the protein-purification unit tracker in myPLTW for Activity 4.1.3 GFP Protein Purification and read the purification overview notes.
Mark the purification-overview entry complete and attach your exit ticket.
Cloning unit should be fully closed; this overview opens the protein-purification unit.
Exit ticket defining purity and one impurity risk submitted to the course shell.
All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.
Today's PLTW tracker
Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.
Use the code Mr. Mendoza gave you, not your name. Saved on this device.
GFP, chromatography, SDS-PAGE / gel interpretation, purity and QC. · Purification overview
Open the protein-purification unit tracker in myPLTW for Activity 4.1.3 GFP Protein Purification and read the purification overview notes.
Cloning unit should be fully closed; this overview opens the protein-purification unit.
This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.
🎯 Explain why a manufactured protein must be purified and what purity means for a medicine.
- Read the purification overview notes in the PLTW course shell and define purity.
- List what else is in the cell mixture besides the target protein.
- Explain why an impure protein medicine could be unsafe.
- Preview the purification steps you will model this week.
- Submit a short exit ticket defining purity and one risk of impurity.
Exit ticket: Short exit ticket defining purity and identifying one concrete safety risk of an impure protein medicine.
Submit on SchoologyUpload by 11:29 PM for full credit.
| Task | Who |
|---|---|
| Read the purification overview notes in the PLTW course shell and define purity. | _______ |
| List what else is in the cell mixture besides the target protein. | _______ |
| Explain why an impure protein medicine could be unsafe. | _______ |
| Preview the purification steps you will model this week. | _______ |
| Submit a short exit ticket defining purity and one risk of impurity. | _______ |
Working solo? Put your own name in "Who" for every row.
- You'll be able to explain why a protein medicine must be purified.
- You'll be able to define purity and a risk of impurity.
Teacher-posted resources
Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched Protein purification and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:protein purification, gfp, chromatography. Score 150. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched Protein purification and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:protein purification, gfp, chromatography. Score 150. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched Protein purification and quality control by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:gfp, chromatography. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.
Lab & supplies
WebXam practice
Cumulative WebXam review
A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.
Where this leads — careers
What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.
What to do if you were absent
Today is individual PLTW work, so do exactly what we did in class, from home: complete the same PLTW target above, then submit your Exit ticket.
Open Schoology (CMSD) and keep goingHow to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.
Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:
Genetic Science Learning Center: Genetics basics and proteinsOptional extra credit (async)
You've passed Unit 2, so the optional extra-credit track is open. Complete reserved-unit work from home (virtual labs included) for extra credit, all submitted on Schoology.
Open the extra-credit track- CompleteEvery required part of the artifact is present, nothing left blank.
- AccurateThe science and the data are correct and match the evidence.
- Scientific reasoningYou explain your claim with evidence and reasoning (CER), not just an answer.
- Professional communicationClear, organized, labeled, and written the way a clinician or scientist would.
- SubmittedTurned in the right way (Schoology for routine work) and confirmed.
Drop your Wed, Apr 28, 2027 · Purification overview here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).
Upload a project