John Hay
John Hay Biomedical
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Tue, Apr 27, 2027Spring (Semester 2) · Week 15Day 63 of 6780-min block

Cloning workflow quiz

Today's target

Demonstrate mastery of the cloning and expression workflow on the weekly quiz.

Due today · WebXam practice Required

Completed cloning workflow quiz using plasmid, ligase, transformation, and expression vocabulary with correctly ordered workflow steps.

Your 4 steps today
  1. 1
    Do this
    Demonstrate mastery of the cloning and expression workflow on the weekly quiz.
  2. 2
    Use this resource
    Open the posted files in the Resources section below
  3. 3
    Submit this
    WebXam practice: Completed cloning workflow quiz using plasmid, ligase, transformation, and expression vocabulary with correctly ordered workflow steps.
  4. 4
    Submit it here
    1. 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
    2. 2Clever. Clever opens. Sign in if it asks.
    3. 3Microsoft (district) login. Use your district Microsoft account (the one for school).
    4. 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
    The file to submit is named: Genetics of Disease (Medical Interventions) › Plasmids, restriction enzymes, ligase, transformation, protein expression. › WebXam practice
    Open Schoology
Were you absent? Jump to the make-up plan
Open PLTW / submit on Schoology Guided notes PDF If you were absent
AgendaPLTW WorkDaily TrackerResourcesLab & SuppliesVocabularyWebXamCareersAbsentExtra Credit
Where this fits
Tested on (Ohio WebXam)
Genetics of Disease · 072130
PLTW lesson
MI · Cloning workflow quiz
WebXam domain
Bio-Molecular Technology
Evidence to produce
WebXam practice
Lab / skill
Genetic Science Learning Center: Cloning
Quick glossary
CER:
Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
SOP:
Standard Operating Procedure — the exact steps to follow (especially in a lab).
Tracker:
Your PLTW progress log where you record completed evidence.
myPLTW:
The PLTW course site where you do the online activities — you open it through Schoology.
Learn first

Minute-by-minute · 80-minute block

💡 Big idea: Mastery of the cloning workflow means being able to sequence, explain, and troubleshoot each step from plasmid to protein.

  1. 0-15Silent review: cloning-tools diagram, workflow data, expression notes
  2. 15-20Open quiz in PLTW course shell; read all items before answering
  3. 20-55Complete quiz items; use required vocabulary for every response
  4. 55-65Check ordered-step questions against workflow sequence
  5. 65-72Final review pass before submit
  6. 72-80Submit quiz; note any items to revisit for WebXam prep
Mr. Mendoza's 5-minute intro
  • • Today is the knowledge checkpoint for everything you did this week.
  • • The quiz will ask you to sequence steps, apply vocabulary, and reason about results.
  • • Spend the first 15 minutes reviewing before you open the quiz.
  • • The cloning workflow appears in the Molecular and Genetic Technology and Lab SOPs domains of the WebXam.
Do this, step by step
  1. 1Review your cloning-tools diagram, workflow data, and expression notes.
  2. 2Open the cloning workflow quiz in the PLTW course shell.
  3. 3Answer each item using the terms plasmid, ligase, transformation, and expression.
  4. 4Check that any ordered-step questions follow the correct workflow sequence.
  5. 5Submit your completed cloning workflow quiz.
You'll be able to
  • • You'll be able to sequence the cloning workflow correctly.
  • • You'll be able to apply cloning vocabulary to quiz scenarios.
Know by the end
  • • The canonical workflow is: cut, insert, ligate, transform, select, express, purify.
  • • Each step has a required tool and a failure mode worth knowing.
  • • Vocabulary precision on ordered-step questions separates partial from full credit.
📺 Tutor me: Learn.Genetics: Cloning
Do the work

Your PLTW work today

Open this PLTW section today

Plasmids, restriction enzymes, ligase, transformation, protein expression. · Cloning workflow quiz

Day 5 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.

Do this: Open the cloning workflow quiz in myPLTW and confirm all cloning-unit evidence slots are filled before submitting.

Complete

Mark the quiz entry complete after submission.

How far to get

Expression notes should be done (Thursday); cloning unit fully closed today.

Upload as evidence

Quiz submission confirming the cloning-unit evidence is complete.

All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.

The plan

Today's PLTW tracker

Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.

Use the code Mr. Mendoza gave you, not your name. Saved on this device.

Plasmids, restriction enzymes, ligase, transformation, protein expression.Day 5 of this projectSee the full week plan
Today's PLTW target

Plasmids, restriction enzymes, ligase, transformation, protein expression. · Cloning workflow quiz

Open the cloning workflow quiz in myPLTW and confirm all cloning-unit evidence slots are filled before submitting.

Expression notes should be done (Thursday); cloning unit fully closed today.

This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.

1 · What you do today

🎯 Demonstrate mastery of the cloning and expression workflow on the weekly quiz.

  • Review your cloning-tools diagram, workflow data, and expression notes.
  • Open the cloning workflow quiz in the PLTW course shell.
  • Answer each item using the terms plasmid, ligase, transformation, and expression.
  • Check that any ordered-step questions follow the correct workflow sequence.
  • Submit your completed cloning workflow quiz.
2 · Turn in today

WebXam practice: Completed cloning workflow quiz using plasmid, ligase, transformation, and expression vocabulary with correctly ordered workflow steps.

Submit on Schoology

Upload by 11:29 PM for full credit.

Where to find it
Open the posted files in the Resources section below
3 · Who's doing what (team)
TaskWho
Review your cloning-tools diagram, workflow data, and expression notes._______
Open the cloning workflow quiz in the PLTW course shell._______
Answer each item using the terms plasmid, ligase, transformation, and expression._______
Check that any ordered-step questions follow the correct workflow sequence._______
Submit your completed cloning workflow quiz._______

Working solo? Put your own name in "Who" for every row.

4 · Words I can use correctly
5 · I'm successful today when I can…
  • You'll be able to sequence the cloning workflow correctly.
  • You'll be able to apply cloning vocabulary to quiz scenarios.
6 · Reflection & next steps
Where are you today?0/7 checked
Pick your period and code first.
Explore

Teacher-posted resources

Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.

Catch-up / reteachFor: Need extra support
pGLO Bacterial Transformation Quick Guide
worksheet/handoutOpens here
Open the file

Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.

Placement rationale

Matched Recombinant DNA and cloning workflow by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:transformation, pglo. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

Catch-up / reteachFor: Need extra support
Lesson 4.1 pGLO Workflow Graphic
worksheet/handoutOpens here
Open the file

Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.

Placement rationale

Matched Recombinant DNA and cloning workflow by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:transformation, pglo. Score 138. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

Catch-up / reteachFor: Need extra support
Activity 4.1.2 pGLO Transformation Kit Quick Guide
worksheet/handoutOpens here
Open the file

Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.

Placement rationale

Matched Recombinant DNA and cloning workflow by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:transformation, pglo. Score 138. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).

How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.

Lab day

Lab & supplies

Bring / set up
Plasmid and gene-insert models or DNA simulation kitRestriction enzyme and ligase reagents or model cardsMicropipette and tipsHost cell transformation simulation materialsSelection plate reference handoutSafety goggles and nitrile gloves
Genetic Science Learning Center: Cloning
Words

This unit's vocabulary

plasmid/PLAZ-mid/recombinant DNAligasetransformation/trans-for-MAY-shun/expression

Tap the speaker to hear a term. Weekly vocabulary task: add two of these terms to your notebook glossary with a definition and an example in your own words.

Check yourself

WebXam practice

Tap an answer to check it · nothing is recorded or graded
A plasmid is best described as
Which enzyme is responsible for sealing two pieces of DNA together to form recombinant DNA?
During bacterial transformation, calcium chloride and a heat shock are used to
Transformed bacteria are plated on agar containing an antibiotic because the plasmid also carries an antibiotic-resistance gene. This step
Check yourself

Cumulative WebXam review

A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.

Tap an answer to check it · nothing is recorded or graded
[Review: Molecule to Patient: Unit 2 Synthesis] A genetic counselor's main role on the health care team is to
[Review: When Cells Forget the Rules: Cancer Launch] When cancer cells break away and spread to other areas of the body, this process is called
[Review: From Biopsy to Plan: Treating Cancer] A tumor suppressor gene that cannot correct damage will trigger apoptosis. Apoptosis is
A plasmid is best described as
Explore

Where this leads — careers

What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.

Genetic CounselorHelp families understand inherited risk and testing options.ImmunologistStudy how the body defends itself and design vaccines and therapies.OncologistDiagnose and treat cancer at the cell and DNA level.Biological TechnicianRun molecular and genetic lab techniques in research and biotech.
Safety net

What to do if you were absent

If YOU are absent

Today is individual PLTW work, so do exactly what we did in class, from home: complete the same PLTW target above, then submit your WebXam practice.

Open Schoology (CMSD) and keep going

How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.

If MR. MENDOZA is absent

Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:

Genetic Science Learning Center: Cloning
Explore

Optional extra credit (async)

You've passed Unit 2, so the optional extra-credit track is open. Complete reserved-unit work from home (virtual labs included) for extra credit, all submitted on Schoology.

Open the extra-credit track
How this is graded
For: WebXam practice — Completed cloning workflow quiz using plasmid, ligase, transformation, and expression vocabulary with correctly ordered workflow steps.
  • Complete
    Every required part of the artifact is present, nothing left blank.
  • Accurate
    The science and the data are correct and match the evidence.
  • Scientific reasoning
    You explain your claim with evidence and reasoning (CER), not just an answer.
  • Professional communication
    Clear, organized, labeled, and written the way a clinician or scientist would.
  • Submitted
    Turned in the right way (Schoology for routine work) and confirmed.
Submission Zone

Drop your Tue, Apr 27, 2027 · Cloning workflow quiz here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).

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