Gel map
Fri, May 7, 2027 · Week 16 · Biotechnology for Health (Biomedical Innovations)
Today's goal: Interpret your gel by estimating fragment sizes and confirming the plasmid identity.
This is a model of the work you should turn in today. Use it to check your own: match the structure and the level of detail, do not copy it. Your data and wording should be your own.
Step 1, ladder distances (mm migrated from well):
- 1000 bp band: 14 mm
- 700 bp band: 19 mm
- 500 bp band: 24 mm
- 300 bp band: 31 mm
Step 2, standard curve: I plotted log(fragment size) on the y-axis against migration distance on the x-axis; the points fall on a near-straight line, so I can read sizes between the markers.
Step 3, sample band estimates from the curve:
- Sample band at 22 mm is about 600 bp.
- Sample band at 28 mm is about 380 bp.
Step 4, compare to the predicted restriction map: My map predicted a double digest would give fragments of about 600 bp and 400 bp.
Conclusion: My estimated sizes (about 600 bp and 380 bp) are close to the predicted 600 bp and 400 bp, so the gel is consistent with the expected construct and the plasmid identity is confirmed within the limits of gel estimation.
| Ladder band (bp) | Distance (mm) |
|---|---|
| 1000 | 14 |
| 700 | 19 |
| 500 | 24 |
| 300 | 31 |
This model shows the level of evidence and organization needed to complete: Completes the gel-map step: ladder band migration distances, a standard curve relating distance to fragment size, estimated sample fragment sizes, the predicted restriction-map sizes, and a conclusion on plasmid identity.
- Name the variables and include units.
- Enter observations without changing the raw values.
- Check labels, calculations, and patterns before interpreting the data.
Keep the structure. Replace the question, facts, measurements, and evidence. Then recheck units, vocabulary, and whether the conclusion goes beyond the evidence.
Also due today: Submit your gel map data table and conclusion on Schoology today.
Turn in: Gel analysis and standard curve
Go to Schoology to turn this in.
Submit one PDF. Put your first and last name in the document header. Name the file: FirstName LastName - Assignment Title - YYYY-MM-DD.pdf.
Open Schoology PDF upload helpIf you cannot get in, see Mr. Mendoza. Do not skip the work.
Claim ceiling: Today's evidence supports a classroom claim about gel map. It cannot prove causation, diagnose a real patient, or justify action outside this room.
One exam-style question that uses exactly what you practiced today. Try it before you reveal the answer, then read why each choice is right or wrong.
| Ladder band (bp) | Distance (mm) |
|---|---|
| 700 | 19 |
| 500 | 24 |
| Unknown | 22 |
Tap an answer to see the full explanation. Nothing is recorded or graded.
It builds this reusable test skill: Estimating an unknown fragment size from a DNA ladder standard curve.
- Name the concept or data pattern being tested.
- Cross out choices that violate that rule or the evidence.
- Justify the best remaining choice before checking the answer.