Exit Ticket: Problem 6 Transformation & Gel (Final check)

The next check on this week's work. It revisits a skill you have already seen, so treat it as another go rather than new ground. Enter your Student ID and submit.

1. 1. You cut a circular plasmid with two restriction enzymes. Each one cuts it once, at unevenly spaced sites. How many bands appear on the gel?

   AOne bandBThree bandsCFour bandsDTwo bands

2. 2. Half the cells from your transformation tube go on plain LB agar. The other half go on LB with ampicillin. The plain LB plate is there to show:

   AThat the cells survived the procedureBWhich cells took up the plasmidCThat the ampicillin plate was poured correctlyDHow fast colonies grow at 37 degrees C

3. 3. To grow a pure culture from your transformation plate, you should pick:

   AA scrape from where colonies run togetherBOne colony that is well separatedCSeveral colonies at onceDA sample of the liquid left on the plate

4. 4. A complete digest of a 5000 base-pair plasmid gives a 3500 base-pair band. The rest of the plasmid totals ____ base pairs.

   A3500B8500C1500D5000

Enter your Student ID to submit.