Exit Ticket: Problem 6 Transformation & Gel (After your intervention)

The next check on this week's work. It revisits a skill you have already seen, so treat it as another go rather than new ground. Enter your Student ID and submit.

1. 1. A DNA ladder is run alongside your samples on a gel so you can:

   AEstimate the size of your fragmentsBStain the DNA so the bands show upCRead the DNA's base sequenceDPull the DNA through the gel

2. 2. You are counting transformed colonies. Which plate can you report a colony count from?

   AA plate with a solid lawn of growthBA plate with about 100 separate coloniesCA plate with growth smeared in one streakDA plate with colonies merged into patches

3. 3. An antibiotic plate grows small white colonies plus one large fuzzy green spot. The green spot is most likely:

   ACells that mutated to resist the drugBA patch where the antibiotic ran outCMold that landed on the plateDA very large E. coli colony

4. 4. On a finished gel, the band that traveled farthest from the well holds:

   AThe largest fragmentBThe uncut plasmidCThe most copies of DNADThe smallest fragment

Enter your Student ID to submit.