Exit Ticket: Problem 6 Transformation & Gel (Initial check)

A quick check on today's hinge idea. Enter your Student ID, answer each item, and submit. You will see your result right away. This is practice, not a grade.

1. 1. Bacteria are plated on agar containing an antibiotic. Colonies grow only where cells took up the plasmid. The plasmid part that lets them survive is:

   AAn origin of replicationBExtra nutrients the cells can useCA gene for a glowing marker proteinDAn antibiotic-resistance gene

2. 2. In a transformation lab you also spread untransformed cells on an antibiotic plate. What does that plate check?

   AHow many cells took up the plasmidBThat the antibiotic is workingCHow well the transformation workedDThat the cells are competent

3. 3. Transformation plates go into a 37 degree C incubator overnight. Why that temperature?

   AIt is close to where E. coli grows bestBIt softens the agar for spreadingCIt matches the temperature of the heat shockDIt activates the plasmid's resistance gene

4. 4. DNA moves toward the positive end of a gel because DNA is:

   APushed through the pores of the agaroseBLighter than the buffer around itCNegative because of its phosphate groupsDPulled along by the loading dye it is mixed with

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