Exit Ticket: Problem 6 Molecular Biology Workflow (After your intervention)

The next check on this week's work. It revisits a skill you have already seen, so treat it as another go rather than new ground. Enter your Student ID and submit.

1. 1. DNA ligase's job in building a recombinant plasmid is to:

   ACopy the plasmid inside the bacteriaBCut the plasmid at its restriction siteCSeal the insert into the cut plasmidDCarry the plasmid into the cell

2. 2. After the heat shock and ice step, cells sit in nutrient broth for 30 minutes before plating. That time lets them:

   ABuild the resistance proteinBTake up more plasmid DNACGrow into colonies before platingDCool slowly back to room temperature

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