Principles of Biomedical Technology (Principles of Biomedical Science)
Unit 3: Unit 3.1 Nosocomial NightmarePBS 3.1Handling, Preparation, Storage and Disposal

Clean immersion oil off the objective

The materials list puts lens wipes next to the oil for a reason. Learn which objective the oil is for, what a paper towel does to a coated lens, and why oil left overnight is a different problem.

Builds on (2 levels back)curriculum · high confidence
  • Only one objective is built for oil: The 100x objective is made to work with its front lens in oil. The others are not, and they are not made to be cleaned of it either.
  • A lens surface is coated and soft: Objective lenses carry thin coatings. Anything abrasive drawn across one leaves marks that stay in every image the instrument makes afterwards.

Prerequisites are inferred: pending teacher review.

Re-learn the skill with worked practice and clear examples.

Now handle the two mistakes that cost a microscope rather than a slide.

Step 1: Do not swing a dry lens through oil
Rotating the turret after the oil is on drags whichever objective comes next straight through it. That lens was never meant to meet oil and is hard to clean.
Step 2: Nothing but lens tissue touches a lens
Paper towel, tissue from a bag and clothing all carry fibres and grit that are abrasive at the scale of a coated lens. A scratch is permanent.
Step 3: If you have no wipes, stop
There is no acceptable substitute, so a missing box of wipes is something to report rather than something to solve with whatever is nearest.
Practice

A student finishes at 100x under oil and rotates the 40x objective round for another look. What is wrong with that?

Reviewed
  1. A.The slide will be pushed out of position by the larger lens
  2. B.The 40x gives a lower magnification, so it is a waste of time
  3. C.Nothing at all, since the 40x objective simply will not focus through oil anyway
  4. D.The dry objective is dragged through oil it was never built for
Show the worked solution ▾

Answer: D. The dry objective is dragged through oil it was never built for

  1. Step 1: Ask what the turret does: Swings the next objective across the same spot the last one was in.
  2. Step 2: Ask what is sitting in that spot: The oil you just put there.

Why it's right: Turning the turret sweeps the next lens through whatever is on the slide, and right now that is oil. The dry objective picks it up, and it is not a lens built to be cleaned of oil.

Why the others miss:
  • A: The objective does not push the slide about. What it does is collect the oil that is lying there.
  • B: Going back to lower power is a normal thing to want, and the problem is the route the lens takes rather than the magnification it gives.
  • C: Whether it focuses is beside the point. The damage happens on the way past, before anybody looks through it.

Aligned to Handling, Preparation, Storage and Disposal · reading level ~grade 9

There are no lens wipes left at your station. Which of these is an acceptable substitute?

Reviewed
  1. A.The corner of your shirt, which is softer than paper
  2. B.A folded paper towel, so long as a clean part of it is what touches the glass
  3. C.None of them, so tell your teacher rather than improvise on a coated lens
  4. D.A tissue from your bag, used gently
Show the worked solution ▾

Answer: C. None of them, so tell your teacher rather than improvise on a coated lens

  1. Step 1: Ask what they have in common: All three are made of fibres coarse enough to mark a coated lens.
  2. Step 2: Ask what a scratch costs: Every image that microscope makes from now on.

Why it's right: Clean is not the same as safe here. All three substitutes are abrasive at the scale of a lens coating, and a mark left on an objective stays in every image the instrument produces afterwards.

Why the others miss:
  • A: Fabric feels soft to a hand and carries grit and thread ends that a lens coating cannot survive.
  • B: A clean paper towel is still wood fibre, and gentleness does not change what it is made of.
  • D: A tissue is designed for skin, not optics, and the same fibre problem applies however carefully it is used.

Aligned to Handling, Preparation, Storage and Disposal · reading level ~grade 9

Where you'd see this
  • A student lifts the slide straight down and away rather than rotating the turret with oil in place.
Guided notes

Fill these in as you work through the lesson.

Big idea: The oil goes on one objective and comes off with lens wipes before the microscope is put away. Nothing else touches a lens, and no dry objective is dragged through oil.
Key terms: write the meaning
  • Immersion oil (sits between slide and the 100x lens):  
  • Objective (the lens closest to the slide):  
  • Lens wipes (the only thing that touches a lens):  
  • Abrasive (what a paper towel is, at this scale):  
The rule

Immersion oil is for the   objective only. Clean it off with   before the microscope goes away, because oil left on a lens collects   and thickens. Never swing a   objective through the oil.

Check yourself
  1. Say why the lens wipes appear in the materials list. 
  2. Explain what happens if the 40x is rotated through the oil. 
  3. Describe why a paper towel is not a substitute. 
Work one example

You finish with the 100x, lift the slide away, and wipe the front of the objective with a lens wipe until it is dry. The scope goes back with a clean lens, so the next student's first view is sharp.