Principles of Biomedical Technology (Principles of Biomedical Science)
Unit 2: Unit 2.2 Decoding a DiagnosisPBS 2.2Biotechnology Research and Experiments

Read A Codon Chart

Look up mRNA codons on a chart to name the amino acid, the start codon, and the stop codons.

Builds on (2 levels back)inferred · high confidence
  • A codon is three bases: You must read the mRNA in groups of three to use the chart.
  • Reading frame: Where you start reading sets the codons, so the frame must stay consistent.

Prerequisites are inferred: pending teacher review.

Re-learn the skill with worked practice and clear examples.

Practice plain lookups: read a codon, find its row on the chart, and write the amino acid: and recognize a stop codon when you hit one.

Step 1: Match the codon to a row
Take the three-base codon and find the same three letters in the chart's left column.
Step 2: Read across
Write the amino acid listed across from it. UUU = Phenylalanine, UGG = Tryptophan, GCA = Alanine.
Step 3: Spot a stop
If the row says STOP (UAA, UAG, or UGA), the protein ends there: no amino acid is added.
Practice

Using the codon chart, which amino acid does the mRNA codon UUU code for?

Approved
mRNA codonMeaning
AUGMethionine (Met) - START
UUUPhenylalanine (Phe)
UGGTryptophan (Trp)
GCAAlanine (Ala)
UAASTOP
UAGSTOP
UGASTOP
A short codon chart linking mRNA codons to amino acids, the start codon, and stop codons.
  1. A.Phenylalanine (Phe)
  2. B.Stop signal, which adds no amino acid
  3. C.Tryptophan (Trp)
  4. D.Methionine (Met)
Show the worked solution ▾

Answer: A. Phenylalanine (Phe)

  1. Step 1: Find UUU: Locate UUU in the chart's codon column.
  2. Step 2: Read its amino acid: Across from UUU the chart lists Phenylalanine (Phe).

Why it's right: The chart shows UUU codes for Phenylalanine (Phe).

Why the others miss:
  • B: The stop codons are UAA, UAG, and UGA, not UUU.
  • C: Tryptophan is UGG, not UUU.
  • D: Methionine is AUG, not UUU.

Aligned to Biotechnology Research and Experiments: codon lookup · reading level ~grade 9

An mRNA reads AUGGCAUGG. Split it into groups of three starting at the first base, then name what the SECOND group calls for.

Reviewed
  1. A.Tryptophan (Trp), the last group of three bases
  2. B.Methionine (Met), the first group of three bases
  3. C.Phenylalanine (Phe), which does not appear in this strand
  4. D.Alanine (Ala), the middle group of three bases
Show the worked solution ▾

Answer: D. Alanine (Ala), the middle group of three bases

  1. Step 1: Split into threes: AUG, then GCA, then UGG.
  2. Step 2: Count to the second group: The second group of three is GCA.
  3. Step 3: Look it up: GCA calls for alanine.

Why it's right: Splitting AUGGCAUGG into threes gives AUG, GCA, UGG. The second group is GCA, which calls for alanine.

Why the others miss:
  • A: Tryptophan comes from UGG, which is the third group here, not the second.
  • B: Methionine comes from AUG, which is the first group here, not the second.
  • C: Phenylalanine comes from UUU, and UUU never appears in this strand.

Aligned to Biotechnology Research and Experiments · reading level ~grade 9

Where you'd see this
  • In Unit 2.2 Decoding a Diagnosis, this skill turns class evidence into a result another person can check.
Video library
Watch: Read A Codon Chart
How to Read a Codon Chart
Amoeba Sisters · 5 min
Guided notes

Fill these in as you work through the lesson.

Big idea: A codon chart turns three-base mRNA codons into amino acids; AUG is the start codon, and UAA, UAG, and UGA are stop codons that end the protein.
Key terms: write the meaning
  • Codon (three mRNA bases):  
  • Codon chart (the key from codon to amino acid):  
  • Start codon (AUG (Met)):  
  • Stop codon (UAA, UAG, or UGA):  
  • Reading frame (where you start grouping the bases):  
The rule

Read the mRNA in groups of   bases. The codon   means start (Methionine). The codons UAA, UAG, and   all mean stop.

Check yourself
  1. Which codon both starts a protein and codes for methionine? 
  2. Name the three codons that mean stop. 
  3. If you start reading at a different base, why do you get different codons? 
Work one example

Look up UUU on the chart and name the amino acid; then say what UAA does. (Answer: UUU = Phenylalanine; UAA = stop, which ends the protein.)