Principles of Biomedical Technology (Principles of Biomedical Science)
Unit 1: Unit 1.1 to 1.2 Evidence & BiomoleculesPBS 1.1-1.2Biotechnology Research and Experiments

Identify Biomolecules From Indicator Data

Use indicator color changes to identify carbohydrates, proteins, and lipids in evidence samples.

Builds on (2 levels back)inferred · high confidence
  • Use a reference chart: Indicators and microscope features must be compared to a known guide.
  • Structure and function: Students connect visible features to what tissues or molecules do.

Prerequisites are inferred: pending teacher review.

Re-learn the skill with worked practice and clear examples.

Use indicator color changes to identify carbohydrates, proteins, and lipids in evidence samples.

Step 1: Learn the key
Match the indicator to the chart before naming the biomolecule present in the .
IndicatorPositive resultBiomolecule
Benedict after heatorangereducing sugar
Iodineblue-blackstarch
Biuretpurpleprotein
Brown papertranslucent spotlipid
Biomolecule indicator chart
Step 2: Use the model
Read the figure, table, control, range, or protocol before choosing an answer.
Step 3: Name the limit
Say what the evidence can support and what it cannot prove yet.
Practice

Use the biomolecule chart. Which positive result identifies protein?

Reviewed
IndicatorPositive resultBiomolecule
Benedict after heatorangereducing sugar
Iodineblue-blackstarch
Biuretpurpleprotein
Brown papertranslucent spotlipid
Biomolecule indicator chart
  1. A.Benedict turns orange
  2. B.Iodine turns blue-black
  3. C.Biuret turns purple
  4. D.Brown paper turns translucent
Show the worked solution ▾

Answer: C. Biuret turns purple

  1. Step 1: Find protein row: The chart links protein to Biuret.
  2. Step 2: Read result: Biuret positive is purple.

Why it's right: Purple Biuret indicates protein.

Why the others miss:
  • A: Orange Benedict indicates reducing sugar.
  • B: Blue-black iodine indicates starch.
  • D: Translucent spot indicates lipid.

Aligned to Biotechnology Research and Experiments · reading level ~grade 9

A drop of an unknown liquid is placed on brown paper and left to dry. Twenty minutes later the spot is still see-through and lets light pass. Which biomolecule does that point to?

Reviewed
  1. A.Starch, which would need iodine to show blue-black
  2. B.Protein, which would need Biuret to show purple
  3. C.Lipid, because oils leave a lasting see-through spot
  4. D.Reducing sugar, which would need Benedict and heat
Show the worked solution ▾

Answer: C. Lipid, because oils leave a lasting see-through spot

  1. Step 1: Ask which test was run: Brown paper is the test used for fats and oils.
  2. Step 2: Read the result: Water evaporates and the paper dries clear, but a lipid leaves a spot that keeps letting light through.

Why it's right: The brown paper test is positive for lipid when the spot stays see-through after the liquid has had time to dry.

Why the others miss:
  • A: Starch is read with iodine, and no iodine was used here.
  • B: Protein is read with Biuret reagent, and no Biuret was used here.
  • D: Reducing sugar is read with Benedict solution and heating, neither of which happened.

Aligned to Biotechnology Research and Experiments · reading level ~grade 9

Where you'd see this
  • In Unit 1.1 to 1.2 Evidence & Biomolecules, this skill turns class evidence into a result another person can check.
Video library
Watch: Identify Biomolecules From Indicator Data
Food tests - Glucose test, Benedict test, Starch test, Biuret test, Sudan III test
Science A Plus Global
Guided notes

Fill these in as you work through the lesson.

Big idea: Use indicator color changes to identify carbohydrates, proteins, and lipids in evidence samples.
Key terms: write the meaning
  • Indicator (chemical test that changes if target is present):  
  • Starch (large carbohydrate):  
  • Protein (macromolecule made of amino acids):  
  • Lipid (fat or oil molecule):  
The rule

Match the indicator   to the chart before naming the biomolecule present in the  .

Check yourself
  1. Which indicator was used? 
  2. What color did the sample turn? 
  3. Which biomolecule matches that color? 
Work one example

Iodine turns an unknown blue-black. Use the chart to identify the biomolecule.