Cloning and purification workflow
Carry out a cloning and protein-purification workflow and record results at each step.
Cloning and purification workflow data table recording transformation results, selection counts, fraction data, yield, and a quality observation.
- 1Do thisCarry out a cloning and protein-purification workflow and record results at each step.
- 2Use this resource
- 3Submit thisLab report: Cloning and purification workflow data table recording transformation results, selection counts, fraction data, yield, and a quality observation.
- 4Submit it here
- 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
- 2Clever. Clever opens. Sign in if it asks.
- 3Microsoft (district) login. Use your district Microsoft account (the one for school).
- 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
The file to submit is named: Genetics of Disease (Medical Interventions) › Plasmids, restriction enzymes, ligase, transformation, protein expression. › Lab reportOpen Schoology
- CER:
- Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure — the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities — you open it through Schoology.
Minute-by-minute · 80-minute block
💡 Big idea: Bacterial transformation followed by selection and purification is the core pipeline for producing recombinant proteins.
- 0-10Review protocol; gather materials; confirm lab setup
- 10-30Transformation step: introduce plasmid into host cells per protocol
- 30-45Selection: plate or score cells; count or estimate transformed colonies
- 45-60Purification step: run first isolation; identify fraction with target protein
- 60-72Record yield and quality observation in workflow data table
- 72-80Clean up; submit data table to course shell
- • This is a hands-on bacterial transformation lab.
- • You will introduce recombinant DNA into host bacteria, select for transformed cells, and run a first purification step.
- • Work carefully: contamination at any step ruins downstream results.
- • Lab SOPs and data recording are both scored domains on the 072130 WebXam.
- 1Read the workflow protocol in the PLTW course shell and gather your materials.
- 2Model transformation by introducing the recombinant plasmid into host cells.
- 3Select transformed cells using the provided marker and record how many grew.
- 4Run the simulated purification step and note where the target protein appears.
- 5Record yield and one quality observation in your workflow data table.
- 6Submit your cloning and purification workflow results.
- • You'll be able to carry out transformation, selection, and purification steps.
- • You'll be able to record yield and a quality note from your workflow.
- • Heat shock or electroporation opens pores in bacterial membranes so plasmid DNA can enter.
- • Antibiotic selection kills non-transformed cells; only cells carrying the resistance gene survive.
- • The first purification step separates soluble protein from cell debris before chromatography.
Your PLTW work today
Plasmids, restriction enzymes, ligase, transformation, protein expression. · Cloning and purification workflow
Day 3 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.
Do this: Open Activity 4.1.2 Protein Factories in myPLTW and follow the transformation and selection protocol to model the cloning and purification workflow.
Mark the cloning-workflow entry complete and attach your workflow data table.
Cloning-tools diagram should be done (Tuesday); workflow data table due today.
Cloning and purification workflow data table with transformation results, selection counts, and fraction data submitted.
All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.
Today's PLTW tracker
Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.
Use the code Mr. Mendoza gave you, not your name. Saved on this device.
Plasmids, restriction enzymes, ligase, transformation, protein expression. · Cloning and purification workflow
Open Activity 4.1.2 Protein Factories in myPLTW and follow the transformation and selection protocol to model the cloning and purification workflow.
Cloning-tools diagram should be done (Tuesday); workflow data table due today.
This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.
🎯 Carry out a cloning and protein-purification workflow and record results at each step.
- Read the workflow protocol in the PLTW course shell and gather your materials.
- Model transformation by introducing the recombinant plasmid into host cells.
- Select transformed cells using the provided marker and record how many grew.
- Run the simulated purification step and note where the target protein appears.
- Record yield and one quality observation in your workflow data table.
- Submit your cloning and purification workflow results.
Lab report: Cloning and purification workflow data table recording transformation results, selection counts, fraction data, yield, and a quality observation.
Submit on SchoologyUpload by 11:29 PM for full credit.
| Task | Who |
|---|---|
| Read the workflow protocol in the PLTW course shell and gather your materials. | _______ |
| Model transformation by introducing the recombinant plasmid into host cells. | _______ |
| Select transformed cells using the provided marker and record how many grew. | _______ |
| Run the simulated purification step and note where the target protein appears. | _______ |
| Record yield and one quality observation in your workflow data table. | _______ |
| Submit your cloning and purification workflow results. | _______ |
Working solo? Put your own name in "Who" for every row.
- You'll be able to carry out transformation, selection, and purification steps.
- You'll be able to record yield and a quality note from your workflow.
Teacher-posted resources
Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched Recombinant DNA and cloning workflow by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:transformation, pglo. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched Recombinant DNA and cloning workflow by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:transformation, pglo. Score 138. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched Recombinant DNA and cloning workflow by path:Medical-Interventions/Unit-4_When-Organs-Fail/4.1_Manufacturing-Human-Proteins; keywords:transformation, pglo. Score 138. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.
Lab & supplies
- • Wear nitrile gloves and safety goggles throughout the procedure.
- • Treat all bacterial cultures as BSL-1 organisms: avoid mouth contact and wash hands before leaving lab.
- • Dispose of all biological waste (plates, tubes, tips) in designated biohazard bags.
- • Wipe bench with 10% bleach or 70% ethanol before and after use.
- • Report any spill involving bacterial culture to the teacher immediately.
- • Do not eat, drink, or apply cosmetics in the lab area.
WebXam practice
Cumulative WebXam review
A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.
Where this leads — careers
What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.
What to do if you were absent
Run the virtual transformation and purification workflow linked in the course shell, recording selection counts and where the target protein elutes, then submit your workflow data table.
Learn.Genetics: Bacterial TransformationThen submit your Lab report on Schoology.
Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:
Genetic Science Learning Center: CloningOptional extra credit (async)
You've passed Unit 2, so the optional extra-credit track is open. Complete reserved-unit work from home (virtual labs included) for extra credit, all submitted on Schoology.
Open the extra-credit track- CompleteEvery required part of the artifact is present, nothing left blank.
- AccurateThe science and the data are correct and match the evidence.
- Scientific reasoningYou explain your claim with evidence and reasoning (CER), not just an answer.
- Professional communicationClear, organized, labeled, and written the way a clinician or scientist would.
- SubmittedTurned in the right way (Schoology for routine work) and confirmed.
Drop your Fri, Apr 23, 2027 · Cloning and purification workflow here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).
Upload a project