Protocol and data stations
Wed, Dec 2, 2026 · Week 15 · Genetics of Disease (Medical Interventions)
Today's goal: Rotate through hands-on stations modeling protein purification, HLA matching, and tissue-engineering data.
This is a model of the work you should turn in today. Use it to check your own: match the structure and the level of detail, do not copy it. Your data and wording should be your own.
Three-station data table on a parallel practice set (made-up numbers, not today's stations):
- Purification station, practice column for a different protein, six fractions: signal 0.70, 0.45, 0.10, 0.03, 0.52, 0.09. Fractions 1 and 2 are flow-through, 3 and 4 are washes, 5 and 6 are elution. Reading: the target is the protein that stayed bound and came off in the elution buffer, so fraction 5 holds it. The high signal in the flow-through is the other proteins in the mixture, not the target.
- Matching station, parallel case: a blood transfusion crossmatch. The recipient's serum was mixed with red cells from two donor units. Unit U-1: no clumping, so the crossmatch is negative and the unit can be given. Unit U-2: clumping, so the crossmatch is positive: the recipient already has antibodies against those cells, and that unit is not given. A crossmatch is read positive or negative; it is not a score. A count such as '5 of 6 HLA matches' is a match count, a different measurement.
- Scaffold station, practice: Scaffold X (fine fibers, small pores) reached 30 percent cell coverage at day 7; Scaffold Y (coarse fibers, large pores) reached 64 percent. Reading: larger pores let cells and nutrients move deeper, which fits Y's higher coverage, but one run per scaffold cannot rule out a difference in how many cells were seeded.
(Tip: write the number in every cell, even an estimate, and write the reading beside it.)
This model shows the level of evidence and organization needed to complete: Models the three-station data table on made-up practice numbers that are not today's stations: a purification column, a matching test read positive or negative, and a scaffold comparison.
- Name the variables and include units.
- Enter observations without changing the raw values.
- Check labels, calculations, and patterns before interpreting the data.
Keep the structure. Replace the question, facts, measurements, and evidence. Then recheck units, vocabulary, and whether the conclusion goes beyond the evidence.
Also due today: turns in the completed data table in Schoology under the Unit 4 Protocol and Data Stations assignment.
Turn in: Three-station data table: purification fraction results, each HLA crossmatch read positive or negative with what it means, and scaffold comparison notes.
Go to Schoology to turn this in.
Submit one PDF. Schoology attaches your name to your upload; on the page itself, write your period and code. Name the file: FirstName LastName - Assignment Title - YYYY-MM-DD.pdf.
Open Schoology PDF upload helpIf you cannot get in, see Mr. Mendoza. Do not skip the work.
Claim ceiling: Today's evidence supports a classroom claim about today's lesson. It cannot prove causation, diagnose a real patient, or justify action outside this room.
One exam-style question that uses exactly what you practiced today. Try it before you reveal the answer, then read why each choice is right or wrong.
Tap an answer to see the full explanation. Nothing is recorded or graded.
It builds this reusable test skill: Interpreting a chromatography fraction result.
- Name the concept or data pattern being tested.
- Cross out choices that violate that rule or the evidence.
- Justify the best remaining choice before checking the answer.