Wet ELISA lab
Wed, Sep 23, 2026 · Week 5 · Genetics of Disease (Medical Interventions)
Today's goal: Run a real ELISA with positive and negative controls and record the color result for each well.
This is a model of the work you should turn in today. Use it to check your own: match the structure and the level of detail, do not copy it. Your data and wording should be your own.
Control check: my positive control (A1) turned strong color and my negative control (A2) stayed clear, both matching my predictions, so I can trust this run.
Reading the samples: Patient sample 1 turned color (positive); Patient sample 2 stayed clear (negative).
Note: I added reagents in the exact order on my plan and kept the incubation times as written, which kept the binding chain intact.
| Well | Reagent added | Color result |
|---|---|---|
| A1 (pos control) | Antigen + detection | Strong color |
| A2 (neg control) | No antigen + detection | Clear |
| A3 (sample 1) | Sample 1 + detection | Color (positive) |
| A4 (sample 2) | Sample 2 + detection | Clear (negative) |
This model shows the level of evidence and organization needed to complete: A recorded data table of each ELISA well with the reagent added and the color result, a note on whether the controls matched predictions, and a plate photograph.
- Name the variables and include units.
- Enter observations without changing the raw values.
- Check labels, calculations, and patterns before interpreting the data.
Keep the structure. Replace the question, facts, measurements, and evidence. Then recheck units, vocabulary, and whether the conclusion goes beyond the evidence.
Also due today: Bring data table to Thursday's analysis; upload plate photo to portfolio.
Turn in: Wet ELISA data table
Go to Schoology to turn this in.
Submit one PDF. Put your first and last name in the document header. Name the file: FirstName LastName - Assignment Title - YYYY-MM-DD.pdf.
Open Schoology PDF upload helpIf you cannot get in, see Mr. Mendoza. Do not skip the work.
Claim ceiling: Today's evidence supports a classroom claim about wet ELISA lab. It cannot prove causation, diagnose a real patient, or justify action outside this room.
One exam-style question that uses exactly what you practiced today. Try it before you reveal the answer, then read why each choice is right or wrong.
Tap an answer to see the full explanation. Nothing is recorded or graded.
It builds this reusable test skill: Following ELISA procedure order and using controls to validate a run.
- Name the concept or data pattern being tested.
- Cross out choices that violate that rule or the evidence.
- Justify the best remaining choice before checking the answer.