Antigen-antibody and ELISA model
Wed, Sep 23, 2026 · Week 5 · Genetics of Disease (Medical Interventions)
Today's goal: Explain how antigens and antibodies bind and run a model ELISA to see how that binding produces a signal.
What a finished product looks like
This is a model of the work you should turn in today. Use it to check your own: match the structure and the level of detail, do not copy it. Your data and wording should be your own.
Antibody specificity makes the test trustworthy because each antibody binds only its matching antigen, so a strong color signal means the specific target really is present, not some other molecule.
Positive result: Well 1 showed the strongest color and the highest concentration, so it is the positive result; the antigen was present at a high enough level to drive the enzyme color reaction.
| Well | Observed color | Concentration from curve |
|---|---|---|
| Well 1 | Dark blue | 100 ng/mL (positive) |
| Well 2 | Medium blue | 10 ng/mL |
| Well 3 | Light blue | 1 ng/mL |
| Well 4 | Nearly clear | ~0.1 ng/mL (negative) |
Also due today: Bring to Friday's submission session; photograph for portfolio.
WebXam problem for today's skill
One exam-style question that uses exactly what you practiced today. Try it before you reveal the answer, then read why each choice is right or wrong.
Tap an answer to see the full explanation. Nothing is recorded or graded.