Standard curve and lab prep
Tue, Sep 22, 2026 · Week 5 · Genetics of Disease (Medical Interventions)
Today's goal: Build a standard curve from known dilutions and prepare the materials and steps for the ELISA model.
What a finished product looks like
This is a model of the work you should turn in today. Use it to check your own: match the structure and the level of detail, do not copy it. Your data and wording should be your own.
How to read an unknown off this curve: find the unknown's signal on the y-axis, trace horizontally until you hit the best-fit line, then drop straight down to read its concentration on the x-axis.
Procedure (in order) for tomorrow:
1. Label wells for each dilution step and the unknown.
2. Add the dilution series to its wells.
3. Add the detection reagent and wait the set time.
4. Record the color in each well.
5. Match each color to a concentration using the curve.
Prediction: a strongly positive sample would fall high on the curve, near the most concentrated standards.
Also due today: Keep graph in notebook; bring the well layout to Thursday's lab session.
WebXam problem for today's skill
One exam-style question that uses exactly what you practiced today. Try it before you reveal the answer, then read why each choice is right or wrong.
Tap an answer to see the full explanation. Nothing is recorded or graded.