Wet ELISA lab
Run a real ELISA with positive and negative controls and record the color result for each well.
Wet ELISA data table: well ID, reagent added, color result; control validation note; plate photograph.
- 1Do thisRun a real ELISA with positive and negative controls and record the color result for each well.
- 2Use this resource
- 3Submit thisData table: Wet ELISA data table: well ID, reagent added, color result; control validation note; plate photograph.
- 4Submit it here
- 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
- 2Clever. Clever opens. Sign in if it asks.
- 3Microsoft (district) login. Use your district Microsoft account (the one for school).
- 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
The file to submit is named: Genetics of Disease (Medical Interventions) › Reading qualitative vs. quantitative color results; false positive/negative risk; control logic. › Data tableOpen Schoology
- CER:
- Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure — the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities — you open it through Schoology.
Minute-by-minute · 80-minute block
💡 Big idea: How does following a precise multi-step procedure with proper controls produce a result you can trust?
- 0-8 minDon goggles and gloves; confirm plate layout matches Tuesday's plan before opening any reagent
- 8-25 minAdd reagents to each well in the correct order per the numbered procedure; keep controls in their designated wells
- 25-45 minWait incubation period exactly as written; do not disturb the plate; record start time
- 45-58 minAdd detection reagent; observe and record color in every well as it develops
- 58-68 minPhotograph the plate next to the layout sheet; save image to portfolio folder
- 68-80 minNote whether controls matched predictions; write one sentence validating (or flagging) the run
- • This is the most technically demanding day of the unit; every step you practiced Tuesday exists to make this run succeed.
- • Real clinical ELISA runs in a hospital lab follow the same sequence you are about to use.
- • Work slowly and methodically; speed kills accuracy in wet lab work.
- • Exit goal: a complete data table and a photographed plate with both controls validated.
- 1Put on goggles and gloves and confirm your plate layout matches your plan.
- 2Add reagents to each well in the correct order, keeping controls separate.
- 3Wait the required incubation times exactly as written in the procedure.
- 4Add the detection reagent and record the color that develops in every well.
- 5Photograph the plate next to your layout sheet for your portfolio.
- 6Note whether your positive and negative controls matched your predictions.
- • You will be able to run an ELISA following a procedure safely.
- • You will be able to record well colors accurately.
- • You will be able to check controls to validate a run.
- • ELISA steps must be performed in exact order; adding reagents out of sequence destroys the binding chain and invalidates results.
- • Incubation times are critical: too short and binding is incomplete; too long and background signal builds up.
- • Checking controls immediately after the run tells you whether to trust any of the other results.
Your PLTW work today
Reading qualitative vs. quantitative color results; false positive/negative risk; control logic. · Wet ELISA lab
Day 3 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.
Do this: Open Activity 1.1.5 ELISA (protocol and results) in myPLTW and follow the wet-lab protocol to run your ELISA today.
Record color results for every well and note whether your positive and negative controls matched predictions.
Plate-layout plan should be done (Tuesday); well-color data recorded and photographed today.
Plate photo and color-result data table in notebook and portfolio folder.
All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.
Today's PLTW tracker
Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.
Use the code Mr. Mendoza gave you, not your name. Saved on this device.
Reading qualitative vs. quantitative color results; false positive/negative risk; control logic. · Wet ELISA lab
Open Activity 1.1.5 ELISA (protocol and results) in myPLTW and follow the wet-lab protocol to run your ELISA today.
Plate-layout plan should be done (Tuesday); well-color data recorded and photographed today.
This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.
🎯 Run a real ELISA with positive and negative controls and record the color result for each well.
- Put on goggles and gloves and confirm your plate layout matches your plan.
- Add reagents to each well in the correct order, keeping controls separate.
- Wait the required incubation times exactly as written in the procedure.
- Add the detection reagent and record the color that develops in every well.
- Photograph the plate next to your layout sheet for your portfolio.
- Note whether your positive and negative controls matched your predictions.
Data table: Wet ELISA data table: well ID, reagent added, color result; control validation note; plate photograph.
Submit on SchoologyUpload by 11:29 PM for full credit.
| Task | Who |
|---|---|
| Put on goggles and gloves and confirm your plate layout matches your plan. | _______ |
| Add reagents to each well in the correct order, keeping controls separate. | _______ |
| Wait the required incubation times exactly as written in the procedure. | _______ |
| Add the detection reagent and record the color that develops in every well. | _______ |
| Photograph the plate next to your layout sheet for your portfolio. | _______ |
| Note whether your positive and negative controls matched your predictions. | _______ |
Working solo? Put your own name in "Who" for every row.
- You will be able to run an ELISA following a procedure safely.
- You will be able to record well colors accurately.
- You will be able to check controls to validate a run.
Teacher-posted resources
Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched ELISA lab, controls, diagnosis limits by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/1.1_The-Mystery-Infection; keywords:elisa, lab. Score 138. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched ELISA lab, controls, diagnosis limits by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/1.1_The-Mystery-Infection; keywords:elisa, lab. Score 138. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched ELISA lab, controls, diagnosis limits by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/1.1_The-Mystery-Infection; keywords:elisa, lab. Score 138. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.
Lab & supplies
- • Goggles and gloves are mandatory before any reagent is opened and must stay on until cleanup is complete.
- • Treat all samples as potentially biohazardous: use disposable tips and do not pipette by mouth.
- • Dispose of used tips, plate, and reagents in the designated biohazard waste container.
- • Add stop solution in a fume hood or well-ventilated area if required by the reagent SDS.
- • Spills on skin: rinse immediately with water for 15 minutes; report to teacher; reference the reagent SDS for Section 4.
- • Do not eat, drink, or touch your face in the lab area.
WebXam practice
Cumulative WebXam review
A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.
Where this leads — careers
What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.
What to do if you were absent
If you miss the wet lab, complete a virtual ELISA simulation and interpret the teacher color dataset, then submit which samples were positive and how your controls validated the run.
learn.genetics (Utah) virtual labsThen submit your Data table on Schoology.
Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:
HHMI BioInteractive- CompleteEvery required part of the artifact is present, nothing left blank.
- AccurateThe science and the data are correct and match the evidence.
- Scientific reasoningYou explain your claim with evidence and reasoning (CER), not just an answer.
- Professional communicationClear, organized, labeled, and written the way a clinician or scientist would.
- SubmittedTurned in the right way (Schoology for routine work) and confirmed.
Drop your Thu, Feb 18, 2027 · Wet ELISA lab here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).
Upload a project