Antigen-antibody and ELISA model
Explain how antigens and antibodies bind and run a model ELISA to see how that binding produces a signal.
Model ELISA data table: well ID, observed color, and assigned concentration from standard curve; positive result identified and explained.
- 1Do thisExplain how antigens and antibodies bind and run a model ELISA to see how that binding produces a signal.
- 2Use this resource
- 3Submit thisData table: Model ELISA data table: well ID, observed color, and assigned concentration from standard curve; positive result identified and explained.
- 4Submit it here
- 1CMSD website. Go to clevelandmetroschools.org and click the Clever button.
- 2Clever. Clever opens. Sign in if it asks.
- 3Microsoft (district) login. Use your district Microsoft account (the one for school).
- 4Schoology. Open Schoology, then your class, then Assignments, and find the file named below.
The file to submit is named: Genetics of Disease (Medical Interventions) › Concentration, serial dilution, standard curves, antigen–antibody binding, direct vs. indirect ELISA. › Data tableOpen Schoology
- CER:
- Claim, Evidence, Reasoning — make a claim, back it with evidence, explain your reasoning.
- SOP:
- Standard Operating Procedure — the exact steps to follow (especially in a lab).
- Tracker:
- Your PLTW progress log where you record completed evidence.
- myPLTW:
- The PLTW course site where you do the online activities — you open it through Schoology.
Minute-by-minute · 80-minute block
💡 Big idea: How does the specificity of antibody-antigen binding make an ELISA a reliable diagnostic tool?
- 0-10 minDraw antigen-antibody diagram in notebook; label antigen, antibody, and specific binding site
- 10-20 minRead the ELISA mechanism: binding, enzyme link, color reaction; summarize in two sentences
- 20-55 minRun the model ELISA: apply dilution series to labeled wells; record color in each well as it develops
- 55-65 minUse the standard curve to assign a concentration to each well color; record in data table
- 65-73 minIdentify the positive result well and explain why it represents a positive in writing
- 73-80 minWrite the specificity sentence; compare data table with a partner and resolve any discrepancies
- • Every HIV test, every COVID antibody test, every pregnancy test uses the same antibody-binding principle you are about to run.
- • The model ELISA uses food coloring to simulate the color reaction so the concept is visible without hazardous reagents.
- • Your pre-lab work from Wednesday is your safety net; follow the labeled layout and numbered steps exactly.
- • Exit goal: a complete data table of well colors matched to concentrations using your standard curve.
- 1Draw an antigen and its matching antibody and label the specific binding site.
- 2Read how an ELISA uses that binding plus a color reaction to detect a target.
- 3Run the ELISA model with your dilution series, recording color at each well.
- 4Match each well's color to a concentration using your standard curve.
- 5Identify which model well represents a positive result and explain why.
- 6Write one sentence on how antibody specificity makes the test trustworthy.
- • You will be able to explain antigen-antibody specific binding.
- • You will be able to describe how an ELISA turns binding into a signal.
- • You will be able to read a model ELISA against a standard curve.
- • Antibodies bind only to their specific antigen at the binding site; this lock-and-key specificity prevents cross-reactions.
- • In an ELISA, binding is coupled to an enzyme-linked color reaction: more antigen bound means stronger color signal.
- • The standard curve converts a color intensity reading into a quantitative concentration.
Your PLTW work today
Concentration, serial dilution, standard curves, antigen–antibody binding, direct vs. indirect ELISA. · Antigen-antibody and ELISA model
Day 4 of this lesson. Open this exact section in myPLTW (reached through Schoology), then do the work below.
Do this: Open Activity 1.1.5 ELISA (protocol and results) in myPLTW and begin setting up your standard-curve data table.
Complete the standard-curve data table using your dilution series concentrations.
Antibody diagram should be done (Wednesday); standard-curve table due today.
Standard-curve data table with concentrations and signal values in notebook.
All PLTW activities are completed inside the PLTW course environment — this page only gives direction. Submit producibles on Schoology.
Today's PLTW tracker
Check things off as you work, then submit. This tells Mr. Mendoza how you're doing so he can help the class. It does not replace turning in your producible on Schoology.
Use the code Mr. Mendoza gave you, not your name. Saved on this device.
Concentration, serial dilution, standard curves, antigen–antibody binding, direct vs. indirect ELISA. · Antigen-antibody and ELISA model
Open Activity 1.1.5 ELISA (protocol and results) in myPLTW and begin setting up your standard-curve data table.
Antibody diagram should be done (Wednesday); standard-curve table due today.
This is how Mr. Mendoza sees the class keeping pace with PLTW. Be honest, it only helps if it is accurate.
🎯 Explain how antigens and antibodies bind and run a model ELISA to see how that binding produces a signal.
- Draw an antigen and its matching antibody and label the specific binding site.
- Read how an ELISA uses that binding plus a color reaction to detect a target.
- Run the ELISA model with your dilution series, recording color at each well.
- Match each well's color to a concentration using your standard curve.
- Identify which model well represents a positive result and explain why.
- Write one sentence on how antibody specificity makes the test trustworthy.
Data table: Model ELISA data table: well ID, observed color, and assigned concentration from standard curve; positive result identified and explained.
Submit on SchoologyUpload by 11:29 PM for full credit.
| Task | Who |
|---|---|
| Draw an antigen and its matching antibody and label the specific binding site. | _______ |
| Read how an ELISA uses that binding plus a color reaction to detect a target. | _______ |
| Run the ELISA model with your dilution series, recording color at each well. | _______ |
| Match each well's color to a concentration using your standard curve. | _______ |
| Identify which model well represents a positive result and explain why. | _______ |
| Write one sentence on how antibody specificity makes the test trustworthy. | _______ |
Working solo? Put your own name in "Who" for every row.
- You will be able to explain antigen-antibody specific binding.
- You will be able to describe how an ELISA turns binding into a signal.
- You will be able to read a model ELISA against a standard curve.
Teacher-posted resources
Classroom documents for this lesson. Ones marked “Open the file” open right here; the rest are posted in Schoology. Use the label on each card to choose the right move.
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched ELISA model, dilution, standard curve by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/1.1_The-Mystery-Infection; keywords:elisa, serial dilution, dilution. Score 154. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Open this when the class reaches this activity and use it to complete the required lesson artifact.
Placement rationale
Matched ELISA model, dilution, standard curve by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/1.1_The-Mystery-Infection; keywords:elisa, serial dilution. Score 146. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
Use this if you were absent, got stuck, or need another pass before you submit the lesson artifact.
Placement rationale
Matched ELISA model, dilution, standard curve by path:Medical-Interventions/Unit-1_How-to-Fight-Infection/1.1_The-Mystery-Infection; keywords:serial dilution, dilution. Score 142. Visibility: student-schoology (student-facing resource; link through Schoology rather than local path).
How to get there: open the CMSD website, click Clever, sign in with your Microsoft (district) account, then open Schoology from Clever.
Lab & supplies
- • Goggles and gloves required for the full model run even with food-coloring solutions.
- • Students must follow the labeled well layout exactly; switching wells invalidates the standard curve comparison.
- • Dispose of used pipette tips in the designated waste container, not loose in the trash.
- • Any spill on skin or eyes: rinse immediately with water; report to teacher.
This unit's vocabulary
Tap the speaker to hear a term. Weekly vocabulary task: add two of these terms to your notebook glossary with a definition and an example in your own words.
WebXam practice
Cumulative WebXam review
A quick mixed-review pulling questions from earlier units plus today, so the WebXam material stays fresh.
Where this leads — careers
What today's skills lead to. These are real health-science careers this course builds toward. Tap one to see, on the US Department of Labor's O*NET site, what the job actually involves, what it pays, and how fast it is growing.
What to do if you were absent
If you are away, complete a virtual ELISA simulation and use the teacher color dataset to match wells to concentrations, then submit your interpretation.
learn.genetics (Utah) virtual labsThen submit your Data table on Schoology.
Class still runs. Complete the online activity above (it's self-guided). Need the concept taught without a teacher? Use this authoritative explainer:
HHMI BioInteractive- CompleteEvery required part of the artifact is present, nothing left blank.
- AccurateThe science and the data are correct and match the evidence.
- Scientific reasoningYou explain your claim with evidence and reasoning (CER), not just an answer.
- Professional communicationClear, organized, labeled, and written the way a clinician or scientist would.
- SubmittedTurned in the right way (Schoology for routine work) and confirmed.
Drop your Wed, Feb 10, 2027 · Antigen-antibody and ELISA model here. Use a clear file name (your initials + project). Routine work still goes to Schoology (via the CMSD portal).
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