Wet ELISA lab
Tue, Sep 29, 2026 · Week 6 · Genetics of Disease (Medical Interventions)
Today's goal: Run a real ELISA with positive and negative controls and record the color result for each well.
What a finished product looks like
This is a model of the work you should turn in today. Use it to check your own: match the structure and the level of detail, do not copy it. Your data and wording should be your own.
Control check: my positive control (A1) turned strong color and my negative control (A2) stayed clear, both matching my predictions, so I can trust this run.
Reading the samples: Patient sample 1 turned color (positive); Patient sample 2 stayed clear (negative).
Note: I added reagents in the exact order on my plan and kept the incubation times as written, which kept the binding chain intact.
| Well | Reagent added | Color result |
|---|---|---|
| A1 (pos control) | Antigen + detection | Strong color |
| A2 (neg control) | No antigen + detection | Clear |
| A3 (sample 1) | Sample 1 + detection | Color (positive) |
| A4 (sample 2) | Sample 2 + detection | Clear (negative) |
Also due today: Bring data table to Thursday's analysis; upload plate photo to portfolio.
WebXam problem for today's skill
One exam-style question that uses exactly what you practiced today. Try it before you reveal the answer, then read why each choice is right or wrong.
Tap an answer to see the full explanation. Nothing is recorded or graded.

