Genetics of Disease (Medical Interventions)
Unit 1: Unit 1.2 Culturing & ResistanceMI 1.2Culturing

Explain Resistance Gene Spread

Use culture and antibiotic evidence to explain resistance gene spread.

Builds on (2 levels back)inferred · high confidence
  • Aseptic culture technique: Culture results need uncontaminated samples.
  • Antibiotic selection pressure: Drug exposure can favor resistant bacteria.

Prerequisites are inferred: pending teacher review.

Re-learn the skill with worked practice and clear examples.

Explain how a resistance gene spreads between bacteria by plasmid transfer (conjugation).

Step 1: Read the transfer diagram
A donor cell connects to a recipient through a pilus and passes a copy of its resistance plasmid.
Two bacteria connected by a pilus; a copy of a resistance (R) plasmid passes from a resistant donor to a recipient cell during conjugation.
Step 2: See the key point
The recipient gains the resistance gene WITHOUT reproducing. This is horizontal gene transfer.
Step 3: Name the limit
Even a different species of bacterium can receive the plasmid, so resistance can jump between species.
Practice

Using the diagram, how does the recipient bacterium become resistant WITHOUT reproducing?

Reviewed
Two bacteria connected by a pilus; a copy of a resistance (R) plasmid passes from a resistant donor to a recipient cell during conjugation.
  1. A.It receives a copy of the resistance plasmid from the donor through a pilus
  2. B.It mutates its own chromosome by chance during the photo
  3. C.It is born already resistant from a resistant parent
  4. D.It absorbs the antibiotic and stores it for later
Show the worked solution ▾

Answer: A. It receives a copy of the resistance plasmid from the donor through a pilus

  1. Step 1: Read the figure: A copy of the R plasmid passes across the pilus.
  2. Step 2: Name the process: Gene moves between living cells = horizontal transfer.

Why it's right: In conjugation, the donor passes a copy of its resistance plasmid to the recipient through a pilus, so the recipient gains resistance without reproducing.

Why the others miss:
  • B: The recipient gains the gene by transfer, not a new chance mutation.
  • C: Inheriting from a parent is vertical transfer; the diagram shows transfer between two existing cells.
  • D: The cell gains a gene, not stored antibiotic.

Aligned to Culturing · reading level ~grade 9

Where you'd see this
  • In Unit 1.2 Culturing & Resistance, this skill turns class evidence into a result another person can check.
Video library
Watch: Explain Resistance Gene Spread
What causes antibiotic resistance? - Kevin Wu
TED-Ed · ~5 min
Guided notes

Fill these in as you work through the lesson.

Big idea: Bacteria can spread antibiotic-resistance genes sideways between living cells by passing plasmids, not only by inheritance.
Key terms: write the meaning
  • Plasmid (a small ____ of DNA that can carry a resistance gene):  
  • Conjugation (two bacteria connect by a pilus and pass a ____ of the plasmid):  
  • Horizontal gene transfer (a gene moves ____ living cells instead of parent to offspring):  
  • Vertical inheritance (a gene passes from ____ to offspring):  
The rule

In horizontal gene transfer, a resistant cell passes a   to another living cell, so resistance can spread without the bacteria  .

Check yourself
  1. What is a plasmid, and what can it carry? 
  2. How does conjugation move a resistance gene between two cells? 
  3. Why does horizontal transfer spread resistance faster than inheritance alone? 
Work one example

A resistant E. coli cell sits next to a non-resistant Salmonella cell. Explain how the Salmonella could become resistant without reproducing, and name the process.