Biotechnology for Health (Biomedical Innovations)
Unit 2: Problem 2: Exploring Human PhysiologyBI 2.1Biomedical Innovation: data & statistics

Choosing a sample size

Decide how many people to include so results are trustworthy, and see why a t-test asks whether two group means differ more than chance.

Builds on (2 levels back)inferred · med confidence
  • Sample vs. population: A sample is the people actually measured; choosing its size only makes sense once you know it stands in for a larger population.
  • Variability (spread) in data: More spread among people means you need more of them to see a real difference, so understanding spread comes first.

Prerequisites are inferred: pending teacher review.

Re-learn the skill with worked practice and clear examples.

A larger, well-chosen sample gives more trustworthy results because it represents the whole group better and is less swayed by one unusual person.

Step 1: See why tiny samples mislead
With only 3 people, one unusual reading can swing the whole result, so a difference you see might just be luck.
Step 2: See why larger helps
More people smooth out the odd readings, so a difference you measure is more likely to reflect the whole group.
Step 3: Balance the cost
Bigger is more trustworthy but takes more time and money, so teams choose a size large enough to be convincing yet practical.
Practice

A team tests whether a sports drink lowers heart rate. Plan A measures 4 people; Plan B measures 40 people. Why is Plan B's result more trustworthy?

Reviewed
  1. A.Because measuring more people makes the drink work better
  2. B.Because a larger sample is less swayed by one unusual reading and better represents the group
  3. C.Because 40 is an even number and 4 is small
  4. D.Because a larger sample removes the need to measure heart rate carefully
Show the worked solution ▾

Answer: B. Because a larger sample is less swayed by one unusual reading and better represents the group

  1. Step 1: Picture one odd person: In a group of 4, one strange reading is a quarter of the data and can flip the result.
  2. Step 2: Scale it up: In a group of 40, that same odd reading is diluted, so the result better reflects the whole group.

Why it's right: A larger sample is less affected by any single unusual reading and represents the group better, so Plan B's result is more trustworthy.

Why the others miss:
  • A: Sample size changes how trustworthy the result is, not whether the drink itself works.
  • C: Whether the count is even or odd has nothing to do with trustworthiness.
  • D: A bigger sample still requires careful measurement; it does not replace it.

Aligned to BI 2.1: sample size and reliability · reading level ~grade 9

Where you'd see this
  • A device trial enrolls dozens of patients rather than a handful so reviewers will believe the measured difference is real and not luck.
Video library
Watch: Choosing a sample size
Sampling Methods and Bias with Surveys: Crash Course Statistics #10
CrashCourse · 10 min
Guided notes

Fill these in as you work through the lesson.

Big idea: Sample size is how many people you measure to stand in for a whole group; larger, well-chosen samples give more trustworthy results. A t-test is a test of whether two group means differ more than random luck would explain.
Key terms: write the meaning
  • Sample size (how many people you measure):  
  • Sample (the measured stand-in for the whole group):  
  • t-test (checks if a gap between two means is bigger than random luck would give):  
  • Statistical significance (when a difference is too big to be explained by random luck alone):  
The rule

A   sample gives more trustworthy results because it represents the whole group better. A t-test asks whether two means differ more than would happen just by  .

Check yourself
  1. A team tests a breathing exercise on 4 people and finds a difference. Why should they be cautious about that result? 
  2. In plain words, what question does a t-test try to answer about two groups? 
  3. If two groups' means are very close together, is a t-test more or less likely to call the difference real? 
Work one example

A team compares resting heart rate before and after caffeine. Explain why measuring 30 people instead of 3 makes the comparison more trustworthy, and state in one sentence what a t-test would check about the two means.