Your Dilution and ELISA model report
Genetics of Disease / Medical Interventions | Monday, September 21, 2026
Today is a submission and consolidation day. No solution, plate, reagent or equipment is used, and the report says so. The finished report never claims a solution, an assay, an observation, a measurement or a diagnosis.
Cover block
- Period and code, not your name: ____________________
- Date: ____________________
- Parts included: [ ] Part 1 [ ] Part 2 [ ] Part 3
- Route: [ ] paper handed to Mr. Mendoza [ ] one PDF to the Wk5 Lab report item
Part 1. Your September 17 dilution record
Check your five-row dilution table against Check 1: one given starting row at 1000 units/mL labeled illustrative; four steps after it; each step divides the row above by 10; a calculation and units/mL in every result. Correct any row readably. Tick each Check 1 box you can confirm.
Check 1
- [ ] One given starting row at 1000 units/mL, labeled illustrative
- [ ] Four steps after the stock row, no fifth step
- [ ] Each step uses the row just above it, divided by 10
- [ ] A calculation and units/mL in every result
- [ ] One prediction sentence that uses the printed assumption and does not claim a measured result
Check your one prediction sentence: it uses the printed assumption, it predicts the direction of signal change across your four steps, and it does not say a test produced that result. Tick the Check 1 box for the prediction.
The same blank record, for an unfinished or missing September 17 page
Use 1000 units/mL as your starting concentration; it is illustrative, not measured. Plan 4 1:10 steps after the starting-stock row. Each step uses one part of the preceding sample and nine parts solvent: ten total parts. Resulting concentration = preceding concentration รท 10. Keep units/mL with every result.
| Row | Preceding concentration (units/mL) | Calculation | Resulting concentration (units/mL) |
|---|---|---|---|
| Starting stock, given | Not applicable | Given illustrative input | 1000 |
| Step 1: use starting stock | ____________________ | ____________________ | ____________________ |
| Step 2: use Step 1 | ____________________ | ____________________ | ____________________ |
| Step 3: use Step 2 | ____________________ | ____________________ | ____________________ |
| Step 4: use Step 3 | ____________________ | ____________________ | ____________________ |
Assumption: For this classroom model, assume signal increases with concentration within its useful range. Your prediction is conditional on that assumption. It is not a measured result or a rule for every assay format.
Your sentence: Predict the direction of signal change across your four steps under that assumption. Explain how the planned concentration change supports your prediction.
________________________________________________________________________________
________________________________________________________________________________
This lesson does not supply measured standards or require a standard curve. Your report has no graph. Do not add one.
Part 2. Your September 18 ELISA model record
Check 2
- [ ] Diagram labels antigen, antibody and the antibody's binding site
- [ ] Explanation names selective binding and its connection to a detectable signal
- [ ] Explanation keeps the limit that selective binding does not guarantee an error-free test
- [ ] Four statements, each supported only by its supplied row, ~ kept on Well 4, no observation claimed
- [ ] Comparison names two wells and keeps the printed model relationship explicit
- [ ] Limit names a conclusion the record cannot establish or the reference information it lacks
The same blank record, for an unfinished or missing September 18 page
Part A. Draw and label antigen, antibody, antibody's binding site. The binding site is on the antibody; the contacted part is on the antigen. Epitope is optional detail.
Reference diagram: ANTIBODY [binding site] <--> [contacted part] ANTIGEN
Your diagram:
________________________________________________________________________________
________________________________________________________________________________
________________________________________________________________________________
Your brief explanation: Write one or two sentences: selective binding, its connection to a detectable signal in the color-based ELISA concept, and the limit that selective binding does not guarantee an error-free test.
________________________________________________________________________________
________________________________________________________________________________
Part B. Supplied model descriptions and concentration labels, not observations made or concentrations calculated from a measured curve. Do not substitute units/mL for ng/mL.
| Well ID | Supplied color description | Supplied concentration label | Your short statement supported by this row |
|---|---|---|---|
| Well 1 | Dark blue | 100 ng/mL | ____________________ |
| Well 2 | Medium blue | 10 ng/mL | ____________________ |
| Well 3 | Light blue | 1 ng/mL | ____________________ |
| Well 4 | Nearly clear | ~0.1 ng/mL | ____________________ |
Part C. For this supplied model, darker blue is associated with a higher assigned concentration. This is the model's stated relationship, not a rule for every ELISA format. Some formats have the opposite relationship between concentration and signal.
Your comparison: Name two wells. In one sentence, compare their supplied color descriptions and concentration labels under the stated model relationship. Make clear that you are comparing supplied model labels, not new measurements.
________________________________________________________________________________
These four rows do not establish positive or negative test results. That would require the test's decision rule and known control results. The concentration labels are supplied; you did not calculate them from measured signals or a calibration curve.
Your limit: Write one sentence naming a conclusion this record cannot establish or one kind of reference information that would be needed to check that conclusion. Do not invent a control result, cutoff or experimental procedure to fill the gap.
________________________________________________________________________________
Part 3. Consolidation page
3A. Write three sentences, one for each of the three items below: what the dilution values are and are not; what the four well rows are and are not; what a positive or negative call would need that this report does not supply.
________________________________________________________________________________
________________________________________________________________________________
________________________________________________________________________________
3B. Write one sentence naming the first thing you would check before trusting a record like this one, and why. Choose from the checks below or name your own from your two records.
- Does each step use the row just above it, with the divisor shown?
- Does every result carry its unit?
- Is the given input labeled illustrative, so no one mistakes it for a measurement?
- Are labels kept separate from observations?
- Before any positive or negative call, are the decision rule and the control results present?
________________________________________________________________________________
3C. Fill in the cover fields: period and code, today's date, the parts-included boxes for Parts 1, 2 and 3, and the route box for paper or one PDF. The cover block is at the top of this page.
Submit once
Complete one Dilution and ELISA model report, Parts 1 to 3. Hand the paper report to Mr. Mendoza before leaving, or submit one PDF to Wk5 Lab report: Dilution and ELISA model submission by the due time shown in Schoology. Use your period and code. Do not submit twice, and do not upload the September 17 or 18 work to its old assignment again. Keep notebook pages in your notebook.