Your ELISA model-analysis record
Genetics of Disease / Medical Interventions | Friday, September 18, 2026
Complete Parts A-C together. This is paper analysis of supplied illustrative information. You are not running an assay, measuring a signal, making a calibration curve or testing a person.
Part A: explain the conceptual model
Using the supplied reference, draw and label antigen, antibody and the antibody's binding site. Keep the binding site on the antibody. The epitope term is optional detail.
Your diagram:
________________________________________________________________________________
________________________________________________________________________________
________________________________________________________________________________
Your brief explanation: In one or two sentences, explain selective binding and its connection to a detectable signal in the color-based ELISA concept. Include the limit that selective binding does not guarantee an error-free test.
________________________________________________________________________________
________________________________________________________________________________
Part B: report the four supplied rows
These are supplied model descriptions and concentration labels, not observations you made or concentrations you calculated from a measured curve.
For each well, write one short statement directly supported by that row's supplied color or concentration label. Sentence fragments are acceptable. Do not invent a measurement or a positive/negative result.
| Well ID | Supplied color description | Supplied concentration label | Your short statement supported by this row |
|---|---|---|---|
| Well 1 | Dark blue | 100 ng/mL | ______________________________ |
| Well 2 | Medium blue | 10 ng/mL | ______________________________ |
| Well 3 | Light blue | 1 ng/mL | ______________________________ |
| Well 4 | Nearly clear | ~0.1 ng/mL | ______________________________ |
The ~ symbol means approximately. Keep that qualification on Well 4's label. The unit ng/mL means nanograms per milliliter. Do not substitute yesterday's units/mL. These are separate supplied model labels, not results from Thursday’s dilution plan, even where the numbers look similar. You may enlarge the response cells in your document or continue below the table while keeping the well IDs.
Part C: compare and state one limit
Model relationship: For this supplied model, darker blue is associated with a higher assigned concentration. This is the model's stated relationship, not a rule for every ELISA format. Some formats have the opposite relationship between concentration and signal.
Your comparison: Name two wells. In one sentence, compare their supplied color descriptions and concentration labels under the stated model relationship. Make clear that you are comparing supplied model labels, not new measurements.
________________________________________________________________________________
________________________________________________________________________________
Interpretation boundary: These four rows do not establish positive or negative test results. That would require the test's decision rule and known control results. The concentration labels are supplied; you did not calculate them from measured signals or a calibration curve.
Your limit: Write one sentence naming a conclusion this record cannot establish or one kind of reference information that would be needed to check that conclusion. Do not invent the missing information or propose a laboratory procedure.
________________________________________________________________________________
________________________________________________________________________________
Check and submit once
- My diagram has the three required labels and a brief explanation with its limit on certainty.
- I wrote one supported statement for each of the four wells.
- My comparison names two wells and keeps the stated model relationship explicit.
- My limit identifies what this record cannot establish or what reference information is missing.
- Parts A-C stay together as one submission; the example and guided notes are not included as extra assignments.
Submit one PDF to Wk4 Data table: Antigen-antibody and ELISA model by September 18 at 11:59 PM. If I collected this work on paper in class, do not upload a second copy. This is 5 points in Daily Work.
The required reading, reference diagram and separate reasoning example are linked through today's portal lesson. Guided notes are optional support.